Transplantation of chronic wounds with epidermal sheets derived from autologous hair follicles—the Leipzig experience

Abstract

Autologous keratinocyte transplantation with EpiDex can be performed easily and safely in patients with chronic wounds with satisfying results. Our data suggest that patients with small ulcer area < 25 cm2 might profit the most from this method.

Development of an Acellular Bioengineered Matrix with a Dominant Vascular Pedicle

“…These have taken the form of acellular templates such as Alloderm (fabricated from decellularized dermis [10]) or Integra (a dermal scaffold made of glycosaminoglycan and collagen [11]), or cellularized scaffolds that have been seeded with various cell types [5][6]12]). The fundamental limitation to all of these constructs is that their survival depends upon host vascular invasion.…”

“…While a number of different techniques have been developed [2][3][4][5][6], one of the most significant and persistent challenges is the development of a microvascular network within the tissue construct that is in direct continuity with a macrovasculature that could be immediately spliced to the host circulation. This macrovascular connection is crucial to allow for surgical anastomosis, and therefore immediate delivery of oxygen to all cells within the construct through its inherent vascular network.…”

Progress of key strategies in development of electrospun scaffolds: bone tissue

“…In this context, electrospun fibers of poly(l-lactide) (PLA) with 20% NBM have shown a higher Young's modulus than pure PLA. These electrospun NBM/PLA composite nanofibers exhibit the properties similar to those of the native collagen-rich mineralized bone matrix and therefore can be used as a temporary substrate for facilitating the isolation and mineralization of bone-forming cells [1].…”

“…TE scaffolding materials have been extensively explored for the regeneration of various parts of the human body, including skin [1], muscle [2], teeth [3], bone [4,5], cartilage [4], ligament [6], spinal cord [7], nerve [8], genome [9] and artificial organs, particularly cardiac tissues or heart [10], lung [11], kidney [12], liver [13], urinary bladder [14], blood vessels [15], stomach [16], intestine [17], breast [18], ear [19], eye [20] and nose [21]. TE includes the combined growth of living cells, tissues or organs, as well as methods and materials that can restrain the shape of a particular tissue for better functions.…”

Wound healing protects against chemotherapy-induced alopecia in young rats via up-regulating interleukin-1β-mediated signaling

“…In mice, wound healing is accelerated during anagen (growth) rather than during telogen (quiescent) phase of the hair cycle (Ansell et al, 2011; Schneider et al, 2009; Stojadinovic et al, 2011). In the clinic, several studies have successfully treated chronic leg ulcers using epidermal equivalents derived from cells of anagen HF outer root sheath, taking advantage of their high proliferation potential (Fox et al, 2016; Jimenez et al, 2015; Limat et al, 1996; Martinez et al, 2016; Ortega-Zilic et al, 2010; Renner et al, 2009; Tausche et al, 2003). On the other hand, it has been known, for decades, that wounding the murine skin can induce the telogen to anagen transition (Hoffmann et al, 1996; Jiang et al, 2010).…”