Transplantation of Cultivated Oral Mucosal Epithelium Prepared in Fibrin-Coated Culture Dishes

Hirayama, Masatoshi; Satake, Yoshiyuki; Higa, Kazunari; Yamaguchi, Takefumi; Shimazaki, Jun

doi:10.1167/iovs.11-7847

Cited by 60 publications

(76 citation statements)

References 29 publications

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“…The usefulness of a serumderived fibrin substrate for cultured corneal epithelial cells has previously been reported (Hirayama et al, 2012;Rama et al, 2001Rama et al, , 2010. As fibrin substrate is absorbed after CLET and COMET, this method has some clinical benefits, in that the cultivated epithelial sheet can be transplanted directly onto the corneal surface.…”

Section: Fibrin and The Temperature-sensitive Dishmentioning

confidence: 99%

Ocular surface reconstruction using stem cell and tissue engineering

Nakamura

Inatomi

Sotozono

et al. 2016

Progress in Retinal and Eye Research

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Section: Fibrin and The Temperature-sensitive Dishmentioning

confidence: 99%

Ocular surface reconstruction using stem cell and tissue engineering

Nakamura

Inatomi

Sotozono

et al. 2016

Progress in Retinal and Eye Research

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“…For example, in a study using rabbit models, transplanted cell sheets enhanced epithelialization of laser-ablated tissues within 5 minutes, leading to complete re-epithelization in 3 to 5 days, while in therapeutic ophthalmology, this technology enabled reduction in corneal haze after excimer laser keratectomy 5 . In addition, a cell sheet derived from a patient s oral mucosal epithelium was transplanted onto their eyes with improved clinical outcomes 6 . In adult dogs with experimentally induced dilated cardiomyopathy DCM , transplanting of a myoblastic sheet onto the left ventricle attenuated myocardial remodeling in 4 weeks with resultant improvement in cardiac function 7 .…”

Section: Discussionmentioning

confidence: 99%

A New Regenerative Approach to Fetal Myelomeningocele by Cell Sheet Transplantation

理絵

Irie

2017

Showa Univ J Med Sci

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: Myelomeningocele MMC is the most common form of congenital neural tube defect. Current fetal surgical repair performed to prevent the exposed spinal cord from being injured until delivery cannot reverse those injuries already in icted in utero.Cell sheet technology has been adopted successfully for the regeneration of diverse organs and tissues, although this promising modality has not yet been used for fetal therapy. This study thus tested our hypothesis that fetal MMC tissue histologically injured in utero could be regenerated using cell sheet technology. We used the L6 myoblast cell line derived from rat skeletal muscle for the cell sheet engineering. A fetal MMC model was also obtained from pregnant Sprague-Dawley SD rats fed orally with retinoic acid 60 mg / kg, embryonic day 10 . Cell sheets were then transplanted onto the fetal MMC lesion embryonic day 19 following maternal anesthesia, laparotomy, and hysterotomy. The incisional wound of the uterus was kept open for 4 hours under anesthesia with the MMC lesion maintained outside the body with the transplanted cell sheet. Subsequently, the experimental fetuses were sacri ced for histological HE stain and immunohistochemical studies to evaluate viability and differentiation potential based on cell markers of the transplanted L6 myoblasts. Immunohistochemical studies clearly demonstrated cell-sheet markers speci c to neurons, skeletal muscle, and myoblasts within the treated MMC lesions, confirming that the cell sheet was biologically implanted within 4 hours after the procedure. Cell sheet technology could be useful for intrauterine regeneration of the fetal rat spinal cord injured by an associated MMC.

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“…However, Hirayama et al [10] compared the clinical results of COMET of substrate-free sheets with those of COMET of amniotic membrane-based sheet on the corneal surface of patients with chemical injury and pseudo-ocular cicatricial pemphigoid. The authors found a better midterm clinical outcome after COMET with a substrate-free cell sheet and concluded that results with amniotic membrane grafts in the presence of chronic inflammatory ocular surface disease are less satisfactory.…”

Section: Choice Of Graft Materialsmentioning

confidence: 99%

Oral Mucosal Grafts: Old Technique in New Light

2013

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Purpose: To present an update on indications and efficacy of oral mucosal grafts in the clinical management of ocular surface diseases focusing on the new developments of the last 5 years. Methods: Pubmed research on the databases of the years 2007 up to 2012 for the key words mucous membrane graft and eye, fornix reconstruction, eyelid reconstruction, and conjunctival replacement. Results: Well-documented clinical indications for oral mucosal grafting to the ocular surface include correction of restricted socket syndrome, ocular adnexal reconstruction after tumor resection, cicatricial ocular surface diseases and therapy-refractory pterygia. New indications are conjunctival insufficiency after filtrating glaucoma surgery and the combination of mucosa and amniotic membrane grafting for fornix reconstruction. Moreover, different strategies for ex vivo mucous epithelial cell expansion are under discussion. Conclusions: Oral mucosal grafting is a viable option for the replacement of the conjunctiva. Advantages include easily accessibility of grafts in sufficient size even for repeated procedures and a high stability of the grafts. On the other hand, nasal mucosal grafts are superior for some indications due to the lack of goblet cells in the oral mucosa.

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Transplantation of Cultivated Oral Mucosal Epithelium Prepared in Fibrin-Coated Culture Dishes

Abstract: A better midterm clinical outcome was achieved with COMET of a substrate-free cell sheet than with COMET of AM as a substrate for treating severe stem cell deficiency.

Cited by 60 publications

References 29 publications

Ocular surface reconstruction using stem cell and tissue engineering

Ocular surface reconstruction using stem cell and tissue engineering

A New Regenerative Approach to Fetal Myelomeningocele by Cell Sheet Transplantation

Oral Mucosal Grafts: Old Technique in New Light

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