Transplantation of Human Fetal Retinal Pigment Epithelium Rescues Photoreceptor Cells from in the Royal College of Surgeons Rat Retina.

Little, Caroline; Castillo, Bienvenido V.; DiLoreto, David A.; Cox, Christian L.; Wyatt, Jeffrey D.; Cerro, Coca del; Cerro, Manuel del

doi:10.1097/00006982-199709000-00030

Cited by 30 publications

(39 citation statements)

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“…The work builds on a background of studies detailing first the progress of photoreceptor loss in the RCS rat (Bourne et al, 1938, Dowling & Sidman, 1962, Cuenca et al, 2005, how cell patterns change or are sustained during degeneration and preservation and what effect photoreceptor loss and survival have on centrally mediated visual function, studied both physiologically and behaviorally (Girman et al, 2005, LaVail 2001, Li & Turner, 1988, Lin et al, 1996, Little et al, 1996, Lund et al, 2001, Sheedlo et al, 1991, Vollrath et al, 2001, Wang et al, 2005.…”

Section: Discussionmentioning

confidence: 99%

Preservation of outer retina and its synaptic connectivity following subretinal injections of human RPE cells in the Royal College of Surgeons rat

Pinilla

Cuenca

Sauvé

et al. 2007

Experimental Eye Research

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We have examined how transplantation of an RPE cell line to the subretinal space of RCS rats affects the distribution of synaptic connectivity markers in the outer plexiform layer of the retina. Using markers of pre-and post-synaptic profiles (bassoon and synaptophysin as presynaptic markers and mGluR6 for postsynaptic profiles) we found that the normal orderly patterns seen between photoreceptors and rod and ON-cone bipolar cells were severely disrupted in dystrophic rats. In areas in which injected cells preserved photoreceptors, more normally appearing pairing of pre-and postsynaptic markers was seen for both rods and cones. The degree of normality correlated with the amount of photoreceptor rescue. The secondary changes that are normally seen in bipolar and horizontal cells were prevented by the photoreceptor preservation. ERG recordings in the animals subsequently studied morphologically showed that both a-and b-waves could be rescued by grafting, albeit with lower amplitudes than normal. Together these anatomical and physiological studies indicate that besides the integrity of outer nuclear layer cells and phototransduction processes, relay circuitry through the outer retina was rescued by cell grafts.

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Section: Discussionmentioning

confidence: 99%

Preservation of outer retina and its synaptic connectivity following subretinal injections of human RPE cells in the Royal College of Surgeons rat

Pinilla

Cuenca

Sauvé

et al. 2007

Experimental Eye Research

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“…This mutation results in profound photoreceptor loss occurring over a severalmonth time period (2,3). Degeneration can be slowed by subretinal injection of normal RPE cells, derived either from allogeneic or xenogeneic sources (4)(5)(6)(7)(8)(9). These results have led to similar transplantation studies in humans with age-related macular degeneration, albeit without success so far (10)(11)(12).…”

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confidence: 99%

“…A second is that little attention has been given to functional parameters in the systematic assessment of graft efficacy (13). Neither levels of photoreceptor rescue (4,(6)(7)(8) nor electroretinogram measurements (5) are indicators of how much vision is restored after intervention.…”

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confidence: 99%

Subretinal transplantation of genetically modified human cell lines attenuates loss of visual function in dystrophic rats

Lund

Adamson

Sauvé

et al. 2001

Proc. Natl. Acad. Sci. U.S.A.

179

118

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“…Other approaches for advanced retinal degeneration which remain experimental at the present time include retinal pigment epithelial (RPE) transplantation [10,63,64,85,104] and photoreceptor (PR) transplantation [7,14,37,65,109]. Gene therapy is extremely promising; however, its potential in advanced retinal dystrophy is currently unknown [1,8,15,47,77,83,110,111,115,118,123].…”

Section: Introductionmentioning

confidence: 99%

A review of in vivo animal studies in retinal prosthesis research

Bertschinger¹,

Beknazar²,

Silva

et al. 2008

Graefes Arch Clin Exp Ophthalmol

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Background The development of a functional retinal prosthesis for acquired blindness is a great challenge. Rapid progress in the field over the last 15 years would not have been possible without extensive animal experimentation pertaining to device design and fabrication, biocompatibility, stimulation parameters and functional responses. This paper presents an overview of in vivo animal research related to retinal prosthetics, and aims to summarize the relevant studies. Methods A Pubmed search of the English language literature was performed. The key search terms were: retinal implant, retinal prosthesis, artificial vision, rat, rabbit, cat, dog, sheep, pig, minipig. In addition a manual search was performed based on references quoted in the articles retrieved through Pubmed. Results We identified 50 articles relevant to in vivo animal experimentation directly related to the development of a retinal implant. The highest number of publications related to the cat (n=18). Conclusion The contribution of animal models to the development of retinal prosthetic devices has been enormous, and has led to human feasibility studies. Grey areas remain regarding long-term tissue-implant interactions, biomaterials, prosthesis design and neural adaptation. Animals will continue to play a key role in this rapidly evolving field.

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Transplantation of Human Fetal Retinal Pigment Epithelium Rescues Photoreceptor Cells from in the Royal College of Surgeons Rat Retina.

Cited by 30 publications

References 0 publications

Preservation of outer retina and its synaptic connectivity following subretinal injections of human RPE cells in the Royal College of Surgeons rat

Preservation of outer retina and its synaptic connectivity following subretinal injections of human RPE cells in the Royal College of Surgeons rat

Subretinal transplantation of genetically modified human cell lines attenuates loss of visual function in dystrophic rats

A review of in vivo animal studies in retinal prosthesis research

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