Transplantation of interscapular brown adipose tissue in rats

Aynehchi, Aydin; Roshangar, Leila; Mahboob, Soltanali; Ahmadiasl, Nasser; Habibi, Parisa; Yousefi, Hadi; Fasihi, Mehdi; Jourabchi-Ghadim, Neda; Ebrahimi-Mameghani, Mehrangiz

doi:10.15421/2018_264

Cited by 3 publications

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“…For histological studies, 5 μm thick sections stained with the H&E method and studied with a Nikon light microscope. For histomorphometric studies, adipose tissue assessment was carried out according to previous studies [26] briefly from each adipose specimen, in 10 randomly selected microscopic fields, a total of 100 crosses sectioned adipose tissue were analyzed and the percentage of each parameter which contained; three types of adipose tissue and connective tissue were averaged for each group using 40X objective lens. One experienced histologist who was blinded to treatment groups assessed the histological examination.…”

Section: Histological Assaymentioning

confidence: 99%

Effects of Royal Jelly and Tocotrienol Rich Fraction in obesity treatment of calorie-restricted obese rats: a focus on white fat browning properties and thermogenic capacity

et al. 2020

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Background: Obesity has reached an alarming rate worldwide. Promoting thermogenesis via increasing the function of brown adipose tissue (BAT) or white adipose tissue (WAT) browning has been proposed as a new protective approach against obesity. The goal of this study was to evaluate the effects of Royal Jelly (RJ) and tocotrienol rich fraction (TRF) on BAT activation and WAT browning during calorie restriction diet (CRD) in obesity model. Methods: In this experimental study, 50 obese Wistar rats were randomly divided into 5 groups and then received one of the following treatments for a period of 8-week: High-fat diet (HFD), CRD, RJ + CRD, TRF + CRD, and RJ + TRF + CRD. Effects of RJ and TRF, individually and in combination on body weight and the expression of key thermoregulatory genes in WAT and BAT were examined by quantitative real-time (qRT-PCR). Also, morphological alterations were assessed by hematoxylin and eosin staining.

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Section: Histological Assaymentioning

confidence: 99%

Effects of Royal Jelly and Tocotrienol Rich Fraction in obesity treatment of calorie-restricted obese rats: a focus on white fat browning properties and thermogenic capacity

et al. 2020

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“…The sections were viewed triplicate by a skilled histologist under the microscope (magni cation, 40X). we conducted histomorphometric studies based on our previous work (30). Actually, 100 sections of each sample of WAT and BAT (10 microscopic elds that were selected randomly and 10 lams were provided from each sample) were assessed and the mean percentage of evaluated adipose tissue (BAT, WAT and beige) and connective tissue were considered for each group.…”

Section: Histological Assaymentioning

confidence: 99%

The Effects of Royal Jelly and Tocotrienol Rich Fraction Along with Calorie Restriction Diet on White Fat Browning and Brown Adipocytes Activation in Obese Rats

Irandoost¹,

Alamdari²,

Saidpour³

et al. 2020

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Background: Obesity is a public health problem across the world. Development of beige adipocytes in white adipose tissue (WAT) and activation of brown adipose tissue (BAT) can support obesity management. We aimed to investigate the effects of royal jelly (RJ) and tocotrienol-rich fraction (TRF) along with calorie restriction diet (CRD) on the genes involved in beige fat formation and BAT activation.Methods: Fifty 3-week-old male Wistar rats were fed high-fat diet (HFD) for 17 weeks. When obesity was induced, they were randomly divided into 5 groups (n=10/group): HFD, CRD, RJ+CRD, TRF+CRD, RJ+TRF+CRD for an additional 8 weeks. Finally, body weight was measured. Moreover, WAT and BAT were dissected for assessing the expression of major genes involved in adipose thermogenesis and histological changes evaluation. Results: At the end of the intervention, weight significantly decreased in RJ and RJ+TRF groups relative to the CRD group (p<0.05). RJ remarkably increased the expression of uncoupling protein 1 (UCP1) by 5.81 and 4.99 times more than CRD alone in WAT and BAT respectively (p<0.001). Expression of peroxisome proliferator-activated receptor-γ coactivator 1α (PGC1-α), peroxisome proliferator-activated receptor-α (PPAR-α) and Sirtuin1 (SIRT1) was significantly increased in WAT and BAT of rats receiving RJ and RJ+TRF. Peroxisome proliferator-activated receptor-γ (PPAR-Ƴ) expression was not noticeably changed in assessed adipose tissues. Brown-like adipocytes in WAT and denser adipocytes in BAT were obvious in RJ and RJ+TRF groups. However, the effect of TRF on studied genes was not noticeable. Conclusion: RJ+CRD improved markers of adipose thermogenesis and induced anti-obesity effects more than CRD alone did. Furthermore, RJ remodeled adipose tissue and could be considered as a new therapeutic target.

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Brown adipose tissue transplantation as a novel alternative to obesity treatment: a systematic review

et al. 2020

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Transplantation of interscapular brown adipose tissue in rats

Cited by 3 publications

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Effects of Royal Jelly and Tocotrienol Rich Fraction in obesity treatment of calorie-restricted obese rats: a focus on white fat browning properties and thermogenic capacity

Effects of Royal Jelly and Tocotrienol Rich Fraction in obesity treatment of calorie-restricted obese rats: a focus on white fat browning properties and thermogenic capacity

The Effects of Royal Jelly and Tocotrienol Rich Fraction Along with Calorie Restriction Diet on White Fat Browning and Brown Adipocytes Activation in Obese Rats

Brown adipose tissue transplantation as a novel alternative to obesity treatment: a systematic review

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