Transport of Amino Acids in Brain Slices

Oja, Simo S.; Vahvelainen, Maija-Liisa

doi:10.1007/978-1-4613-4458-2_3

Cited by 19 publications

(3 citation statements)

References 150 publications

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“…In keeping with several earlier observations, slices from adult mice swelled considerably more than slices from developing The slices from adult and 3-day-old mice were preloaded and superfused as in Figure 1 in normalC1-medium but at 30 min the medium was changed for 20 min to new media of the following compositions: CI--free medium supplemented with 50 mM K + (A), medium which contained I3 mM C1-and 50 mM K + (B), CI--free medium (C), and mice (for references, see Oja and Vahvelainen, 1975). Total swelling was enhanced in high-K+, Cl--free or hyposmotic media and diminished in hyperosmotic media.…”

Section: Discussionmentioning

confidence: 99%

“…The possible reuptake of taurine molecules released from slices must also be taken into account in interpreting the results. Molecules released from the cells in slices must first traverse the extracellular space(s) and escape reuptake in order to emerge finally in superfusion medium (Oja and Vahvelainen, 1975). The measured release thus represents some kind of an "overflow."…”

Section: Discussionmentioning

confidence: 99%

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Taurine release and swelling of cerebral cortex slices from adult and developing mice in media of different ionic compositions

Oja

Saransaari

1992

J of Neuroscience Research

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The release of preloaded radiolabeled taurine was studied in superfused cerebral cortex slices obtained from adult and 3-day-old mice in media of varying ionic composition. Our aim was to establish whether the release of taurine from slices evokable by high concentrations of K+ could be attributed solely to cell volume changes or whether it results directly from depolarization of cell membranes. In both age groups hypoosmotic media enhanced the release of taurine. The enhancement was greater in 3-day-old than in adult mice. The K(+)-evoked release of taurine was likewise greater in slices from 3-day-old mice than in slices from adult mice. The K+ stimulation was totally preserved in adult mice and partially preserved in 3-day-old mice when the slices were superfused with Cl(-)-free media, with media in which the K+ x Cl- ionic product was kept constant and with hyperosmotic high-K+ media. The results were practically the same when the permeant anion acetate and the impermeant anion gluconate were used to replace the Cl- deficit. The unstimulated release of preloaded taurine was greatly enhanced in Cl(-)-free media in both age groups. There obtained no statistically significant correlation between the intracellular swelling of slices and the magnitude of taurine release under the present different experimental conditions in either age group. The results show that the K(+)-evoked release of taurine from superfused cerebral cortex slices cannot be solely attributed to depolarization-induced cell swelling. At least a part of the release results directly from membrane depolarization which besides exocytosis apparently also enhances the carrier-mediated release of taurine and inhibits the reuptake of taurine liberated from intracellular compartments.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Taurine release and swelling of cerebral cortex slices from adult and developing mice in media of different ionic compositions

Oja

Saransaari

1992

J of Neuroscience Research

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“…Uptake at 60°C was low, which indicates that the system could be denatured. Amino acid and sugar transport has been similarly characterised (Albers, 1967;Heinz, 1967;Oja and Vahvelainen, 1975). 'Very high'-affinity uptake of spermine was also strongly sodiumdependent and was inhibited by the presence of raised extracellular concentrations of potassium.…”

Section: Discussionmentioning

confidence: 99%

High‐Affinity Uptake of Spermine by Slices of Rat Cerebral Cortex

Harman

Shaw

1981

Journal of Neurochemistry

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The accumulation of the polyamine spermine into 0.1-mm prisms of rat cerebral cortex has been investigated at both 37 percent C and at 4 percent C. Kinetic analysis of the temperature-sensitive portion of uptake indicates two high-affinity saturable components together with an unsaturable component at high concentrations. The "very high'-affinity saturable system (K(m)= 3.8 nM) was temperature- and sodium-dependent, and significantly reduced by metabolic inhibitors, finding that are consistent with an active transport system for spermine into brain tissue. The "high'-affinity saturable component (K(m)= 0.44 micron) was sodium-dependent and inhibited by ouabain, but only partially susceptible to inhibition by 2,4-dinitrophenol and sodium cyanide. The significance of these results with respect to the function of spermine in the central nervous system is discussed.

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Cell Volume Changes and Taurine Release in Cerebral Cortical Slices

Oja

Saransaari

1992

Advances in Experimental Medicine and Biology

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Transport of Amino Acids in Brain Slices

Cited by 19 publications

References 150 publications

Taurine release and swelling of cerebral cortex slices from adult and developing mice in media of different ionic compositions

Taurine release and swelling of cerebral cortex slices from adult and developing mice in media of different ionic compositions

High‐Affinity Uptake of Spermine by Slices of Rat Cerebral Cortex

Cell Volume Changes and Taurine Release in Cerebral Cortical Slices

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