Transport of deoxy-d-glucose into lymphocytes of patients with polycystic ovary syndrome

Oleszczak, B; Szablewski, Leszek; Pliszka, Monika; Głuszak, Olgierd; Stopińska-Głuszak, U

doi:10.1007/s12020-014-0174-5

Cited by 6 publications

(3 citation statements)

References 37 publications

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“…The quantification of GLUT4 expression on the surface of peripheral blood lymphocytes might be a potentially useful method for the early detection of individuals at a high risk of diabetes. [35] [36] GLUT4 levels in peripheral blood mononuclear cells have been investigated using indirect immunofluorescence in sled dogs, [37] and inflammatory monocyte populations in humans, [38] [39] [40] which could be used as a method to estimate the influence of insulin on GLUT protein translocation and the dynamics of glucose uptake. GLUT4 was also detected in granulocytes and could be used to assess the immune response in diabetes [41] or the response of the plasma membrane to insulin during infection.…”

Section: Discussionmentioning

confidence: 99%

A Potential Role for GLUT4 in Predicting Sepsis in Critically Ill Children

Zhou,

Liu,

et al. 2024

OJIM

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Background: This study investigated serum Glucose transporter (GLUT) 4 levels and examined the relationship between serum GLUT4 levels and sepsis in critically ill children. Methods: This was a retrospective study of 77 critically ill children and 33 non-diabetic healthy children (controls) who were admitted between 07/2015 and 05/2016. Patient data, clinical information, and blood samples were collected on admission, alongside a large number of laboratory parameters that were routinely assessed. Critically ill patients were divided into sepsis and non-sepsis/systemic inflammatory response syndrome (SIRS). Serum GLUT4 was measured using western blotting and enzyme-linked immunosorbent assays. Insulin resistance indexes, clinical data, laboratory parameters, and inflammatory cytokines were assessed. Results: GLUT4 serum levels were higher in critically ill children than in healthy children (90.5 vs. 30.3 µg/L, P < 0.001), and in septic shock compared with sepsis (116.8 vs. 64.3 µg/L, P < 0.05), but not compared to non-sepsis/systemic inflammatory response syndrome (105.7 µg/L, P > 0.05). Compared to healthy children, hyperglycemic patients (n = 48) had elevated GLUT4 serum levels (30.3 vs. 103.7 g/L, P < 0.001). Serum GLUT4 levels were higher in patients who died (n = 16, P < 0.05) than in those who survived (n = 57). Serum GLUT4 levels were positively correlated with the neutrophil count, creatine kinase levels, and glucose levels (P < 0.05). GLUT4 levels for the diagnosis of sepsis had an area under the curve of 0.70 (P = 0.03) when using a 51-µg/L cut-off value, resulting in 74.6% sensitivity and 80% specificity. Conclusions: GLUT4 serum levels might be significantly increased in critically ill children compared with healthy children, particularly those in septic shock. Serum GLUT4 could predict disease severity.

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Section: Discussionmentioning

confidence: 99%

A Potential Role for GLUT4 in Predicting Sepsis in Critically Ill Children

Zhou,

Liu,

et al. 2024

OJIM

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show abstract

“…As such, peripheral blood lymphocytes might become an interesting model system to study the effects of insulin on cellular glucose transport using a ow cytometer. The quanti cation of GLUT4 expression on the surface of peripheral blood lymphocytes might be a potentially useful method for the early detection of individuals at a high risk of diabetes (35,36). GLUT4 levels in peripheral blood mononuclear cells have been investigated using indirect immuno uorescence in sled dogs (37), and in ammatory monocyte populations in humans (38)(39)(40), which could be used as a method to estimate the in uence of insulin on GLUT protein translocation and the dynamics of glucose uptake.…”

Section: Discussionmentioning

confidence: 99%

A Potential Role for GLUT4 in Predicting Sepsis in Critically ill Children

Peng¹,

Liu²,

Pei-qing³

et al. 2020

Preprint

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Background: Glucose transporter (GLUT) 4 is an insulin-sensitive transporter that uptakes blood glucose into muscles and adipose tissue. This study aimed to investigate serum GLUT4 levels in critically ill children and to examine the potential relationship between serum GLUT4 levels and illness severity.Methods: This was a retrospective study of 77 critically ill children and 33 non-diabetic healthy children (controls; routine health check-up) who were admitted between 07/2015 and 05/2016. Serum GLUT4 was measured using western blotting and enzyme-linked immunosorbent assays. Insulin resistance indexes, clinical data, laboratory parameters, and inflammatory cytokines were assessed.Results: GLUT4 serum levels were higher in critically ill children than in healthy children (90.5 vs. 30.3 µg/L, P<0.001), and in septic shock compared with sepsis (116.8 vs. 64.3 µg/L, P<0.05), but not compared to non-sepsis/systemic inflammatory response syndrome (105.7 µg/L, P>0.05). Compared to healthy children, hyperglycemic patients (n=48) had elevated GLUT4 serum levels (30.3 vs. 103.7 g/L, P<0.001). Serum GLUT4 levels were higher in patients who died (n=16, P<0.05) than in those who survived (n=57). Serum GLUT4 levels were positively correlated with the neutrophil count, creatine kinase levels, and glucose levels (P<0.05). GLUT4 levels for the diagnosis of sepsis had an area under the curve of 0.70 (P=0.03) when using a 51-µg/L cut-off value, resulting in 74.6% sensitivity and 80% specificity.Conclusions: GLUT4 serum levels might be significantly increased in critically ill children compared with healthy children, particularly those in septic shock. Serum GLUT4 could predict disease severity in critically ill children.

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“…The objective of the present study was to identify potential links between lncRNA H19 and PCOS. Because PCOS represents a systemic endocrinopathy of the entire body and peripheral blood samples are very appropriate for gene expression studies [26], we deter-mined the expression levels of H19 in peripheral blood leukocytes from PCOS patients and compared this data to that derived from normal controls. We also attempted to correlate the expression of H19 with a range of key clinical parameters in PCOS patients.…”

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confidence: 99%

Long non-coding RNA H19 is associated with polycystic ovary syndrome in Chinese women: a preliminary study

et al. 2019

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Polycystic ovary syndrome (PCOS) represents a serious reproductive and endocrine condition and is associated with high incidence rates. H19 is a compelling long noncoding RNA (lncRNA) which carries out a range of biological functions. However, prior to this study, little was known as to whether there was an association between lncRNA H19 and PCOS. In the current study, we used quantitative real-time polymerase chain reaction (qRT-PCR) to determine lncRNA H19 expression levels in peripheral blood leukocytes from patients with PCOS and compared this data with that derived from normal controls. We also screened data for potential relationships between lncRNA H19 and a range of endocrine variables in PCOS. The expression of lncRNA H19 was significantly higher in cases of PCOS than in controls. Individuals exhibiting higher expression levels of lncRNA H19 were associated with a significantly higher risk of PCOS than those with lower expression levels. Moreover, lncRNA H19 expression was positively correlated with fasting plasma glucose levels; this was the case with both raw data, and after adjustment for age and BMI in the PCOS group. However, lncRNA H19 expression showed no significant correlation with total testosterone or insulin resistance in either PCOS cases or the controls. In conclusion, we demonstrate the first evidence to indicate that lncRNA H19 is associated with PCOS, suggesting that elevated lncRNA H19 levels are a risk factor for PCOS. For susceptible individuals, lncRNA H19 may represent a useful biomarker of the early stages of endocrine and metabolic disorders in PCOS.

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Transport of deoxy-d-glucose into lymphocytes of patients with polycystic ovary syndrome

Cited by 6 publications

References 37 publications

A Potential Role for GLUT4 in Predicting Sepsis in Critically Ill Children

A Potential Role for GLUT4 in Predicting Sepsis in Critically Ill Children

A Potential Role for GLUT4 in Predicting Sepsis in Critically ill Children

Long non-coding RNA H19 is associated with polycystic ovary syndrome in Chinese women: a preliminary study

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