Transport of liposomal and albumin loaded curcumin to living cells: An absorption and fluorescence spectroscopic study

Kunwar, Amit; Barik, Atanu; Pandey, R.; Priyadarsini, K. Indira

doi:10.1016/j.bbagen.2006.06.012

Cited by 258 publications

(190 citation statements)

References 33 publications

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“…Curcumin is a hydrophobic compound owing to its aromatic phenolic groups and methylene bridge [52,53]. The high lipophilicity of curcumin is indicated by its octanol-water partition coefficient of 2.5 Â 10 4 M À1 [54,55]. Curcumin's partition coefficient for 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) lipid bilayers and HEPES buffer was 2.4 Â 10 4 M À1 [56].…”

Section: Discussionmentioning

confidence: 99%

Pharmacological inhibition of lipid droplet formation enhances the effectiveness of curcumin in glioblastoma

Zhang

Cui

Amiri

et al. 2016

European Journal of Pharmaceutics and Biopharmaceutics

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a b s t r a c tIncreased lipid droplet number and fatty acid synthesis allow glioblastoma multiforme, the most common and aggressive type of brain cancer, to withstand accelerated metabolic rates and resist therapeutic treatments. Lipid droplets are postulated to sequester hydrophobic therapeutic agents, thereby reducing drug effectiveness. We hypothesized that the inhibition of lipid droplet accumulation in glioblastoma cells using pyrrolidine-2, a cytoplasmic phospholipase A2 alpha inhibitor, can sensitize cancer cells to the killing effect of curcumin, a promising anticancer agent isolated from the turmeric spice. We observed that curcumin localized in the lipid droplets of human U251N glioblastoma cells. Reduction of lipid droplet number using pyrrolidine-2 drastically enhanced the therapeutic effect of curcumin in both 2D and 3D glioblastoma cell models. The mode of cell death involved was found to be mediated by caspase-3. Comparatively, the current clinical chemotherapeutic standard, temozolomide, was significantly less effective in inducing glioblastoma cell death. Together, our results suggest that the inhibition of lipid droplet accumulation is an effective way to enhance the chemotherapeutic effect of curcumin against glioblastoma multiforme.

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Section: Discussionmentioning

confidence: 99%

Pharmacological inhibition of lipid droplet formation enhances the effectiveness of curcumin in glioblastoma

Zhang

Cui

Amiri

et al. 2016

European Journal of Pharmaceutics and Biopharmaceutics

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“…The phenolic groups of these compounds are chromophoric and fluorescent. 21 Four mechanisms have been proposed by which these compounds bias ThT fluorescence: quenching of ThT fluorescence by absorbing the light for ThT excitation, direct interference with ThT fluorescence by fluorescing in the same region where ThT fluoresces, interactions between ThT and polyphenol, and competition between ThT and polyphenol for the binding sites on the amyloid assemblies. 17 Here, we use 1 H nuclear magnetic resonance (NMR) to determine the kinetic profile of the selfassembly of IAPP in the presence of curcumin and show that it is atypical relative to that of inhibitor-free IAPP in that it includes a prenucleation period.…”

mentioning

confidence: 99%

Kinetic Profile of Amyloid Formation in the Presence of an Aromatic Inhibitor by Nuclear Magnetic Resonance

Liu

Gaines

Robbins

et al. 2012

ACS Med. Chem. Lett.

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The self-assembly of amyloid proteins into β-sheet rich assemblies is associated with human amyloidoses including Alzheimer's disease, Parkinson's disease, and type 2 diabetes. An attractive therapeutic strategy therefore is to develop small molecules that would inhibit protein self-assembly. Natural polyphenols are potential inhibitors of β-sheet formation. How these compounds affect the kinetics of self-assembly studied by thioflavin T (ThT) fluorescence is not understood primarily because their presence interferes with ThT fluorescence. Here, we show that by plotting peak intensities from nuclear magnetic resonance (NMR) against incubation time, kinetic profiles in the presence of the polyphenol can be obtained from which kinetic parameters of self-assembly can be easily determined. In applying this technique to the selfassembly of the islet amyloid polypeptide in the presence of curcumin, a biphenolic compound found in turmeric, we show that the kinetic profile is atypical in that it shows a prenucleation period during which there is no observable decrease in NMR peak intensities. KEYWORDS: amyloid, ThT fluorescence, curcumin, NMR A total of 27 proteins have been identified to form the extracellular β-sheet containing amyloid fibrils associated with human amyloidoses. 1 These include the amyloid-β protein (Aβ) in Alzheimer's disease, α-synuclein in Parkinson's disease, and the islet amyloid polypeptide (IAPP) in type 2 diabetes (T2D). In spite of the large differences in the primary structure of the precursor proteins, the mature fibrils possess common characteristics including unbranched, 10 nm wide morphology as determined by electron microscopy, the ability to bind Congo red and exhibit green birefringence, and X-ray diffraction patterns consistent with cross-β-sheet. 1 Mechanistic studies of fibril formation in hydro have shown that Aβ, α-synuclein, and IAPP undergo a random coil to β-sheet conformational transition. 2 IAPP is a 37-residue polypeptide ( Figure 1A) that is cosecreted with insulin by β-cells of the islets of Langerhans in the pancreas. Molecular biological, biophysical, and genetic evidence support a central role for IAPP in β-cell death and dysfunction associated with T2D. 3,4 The progressive formation of islet amyloid leads to a decrease in β-cell mass. 5 The toxicity of fibrillar IAPP was first demonstrated in the early 1990s, 6 but more recent studies suggest that oligomeric assemblies could be the proximate cytotoxic species in T2D. 3,7 A number of mechanisms behind the toxicity of IAPP oligomers have been proposed, 8 but recent biophysical studies have focused on the ability of IAPP to disrupt model membranes, as reviewed recently. 3 A missense mutation involving the substitution of serine at position 20 with glycine ( Figure 1A) has been linked to an early onset, more severe form of T2D. 9 The mutant polypeptide aggregates faster 10,11 and is more toxic than wildtype IAPP. 10 Together, these findings suggest that molecules that significantly delay or completely inhibit IAP...

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“…The higher curcumin uptake by tumor cells, against normal ones, has been assigned to various hypothetic factors, including the different membrane structure, protein composition and larger size [4]. The anti-oxidant activity of curcumin has been identified as the key mechanism by which this dietary phytochemical prevents cancer in vivo.…”

Section: Introductionmentioning

confidence: 99%

Self-Assembled Dextrin Nanogel as Curcumin Delivery System

Gonçalves¹

2012

JBNB

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Curcumin is a natural polyphenol with anti-oxidative, anti-inflammatory and anti-cancer properties. Its therapeutic potential is substantially hindered by the rather low water solubility and bioavailability, hence the need for suitable carriers. In this study, we show that self-assembled nanogels obtained from hydrophobically modified dextrin are effective curcumin nanocarriers. The stability and loading efficiency of curcumin-loaded nanogel depends on the nanogel/curcumin ratio. Higher stability of the formulation is achieved in water than in PBS buffer, as evaluated by dynamic light scattering and fluorescence measurements. The in vitro release profile, using sink conditions, indicates that dextrin nanogel may perform as a suitable carrier for the controlled release of curcumin. Biological activity of curcumin-loaded nanogel in HeLa cell cultures was assessed using the MTS assay.

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Transport of liposomal and albumin loaded curcumin to living cells: An absorption and fluorescence spectroscopic study

Cited by 258 publications

References 33 publications

Pharmacological inhibition of lipid droplet formation enhances the effectiveness of curcumin in glioblastoma

Pharmacological inhibition of lipid droplet formation enhances the effectiveness of curcumin in glioblastoma

Kinetic Profile of Amyloid Formation in the Presence of an Aromatic Inhibitor by Nuclear Magnetic Resonance

Self-Assembled Dextrin Nanogel as Curcumin Delivery System

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