Transport of mercury compounds across bimolecular lipid membranes: Effect of lipid composition, pH and chloride concentration

Bienvenue, E.; Boudou, A.; Desmazes, J.P.; Gavach, Claude; Georgescauld, D.; Sandeaux, Jacqueline; Sandeaux, Roger; Seta, P.

doi:10.1016/0009-2797(84)90009-7

Cited by 87 publications

(41 citation statements)

References 12 publications

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“…However, chloro-complexes were usually found in higher concentrations in the photocyte and Microtox bioassays than in the P. phosphoreum bioassay that showed greater toxicity. Thus, if chloro-complexes are toxic (Bienvenue et al, 1984;Sarin et al, 2000), the cells in the three bioassays have distinct biological tolerances to these chloro-complexes.…”

Section: Discussionmentioning

confidence: 99%

Chemical speciation and toxicity of metals assessed by three bioluminescence‐based assays using marine organisms

Deheyn

Bencheikh-Latmani

Latz

2004

Environmental Toxicology

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ABSTRACT:Metal toxicity is a function of the biology of the target organism and the chemical speciation of the metal. The toxicity of 11 metals was assessed with three cell-based bioassays based on marine organisms: the bacterium Photobacterium phosphoreum of the Microtoxா bioassay, an environmental strain of P. phosphoreum, and photocytes isolated from the brittlestar Ophiopsila californica. Metal speciation was calculated for three commonly used media: NaCl-based Microtoxா bioassay medium, artificial seawater glycerol, and artificial seawater. Decreased bioluminescence was considered a proxy for cell toxicity. In all three assays the elements Cd and Hg exhibited similar speciation as well as similar toxicity profiles. The element Cu was toxic in all three assays despite different metal speciation for the P. phosphoreum bioassay. The element Ag was toxic to both bacterial strains but not to photocytes despite a similar chemical speciation for all three assays. In general, the Microtoxா bioassay was sensitive to all metals (except Pb), whereas the photocytes were the least sensitive to the metals. The heightened response of the Microtoxா bioassay probably resulted from a combination of the limited complexing power of the medium and the greater sensitivity of the bacterial strain.

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Section: Discussionmentioning

confidence: 99%

Chemical speciation and toxicity of metals assessed by three bioluminescence‐based assays using marine organisms

Deheyn

Bencheikh-Latmani

Latz

2004

Environmental Toxicology

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“…Correlations have been found between the calculated abundance of these lipophilic species with (1) rates of diffusion of mercury across artificial phospholipid membranes (Gutknecht 1981;Bienvenue et al 1984), (2) bioaccumulation and toxicity in a marine algal species (Mason et al 1996), and (3) rates of production of methylmercury in a sulphate reducing bacterium (Benoit et al 2001). A limitation of experiments to date has been the inability to work at the trace concentrations (Յ10 ng L Ϫ1 ) normally found in aquatic environments, because higher levels were required for a detectable response.…”

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confidence: 99%

Evidence for facilitated uptake of Hg(II) by Vibrio anguillarum and Escherichia coli under anaerobic and aerobic conditions

Golding

Kelly

Sparling

et al. 2002

Limnology & Oceanography

116

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A mer-lux bioreporter was used to study uptake of inorganic mercury, Hg(II), at trace concentrations by two facultatively anaerobic bacterial species, Vibrio anguillarum and Escherichia coli. Uptake of Hg(II) by these bacteria appeared to be facilitated, rather than by passive diffusion. Three lines of evidence support this conclusion. First, under anaerobic conditions Hg(II) uptake was greatly decreased compared with aerobic conditions, even though the chemical composition of the medium was identical except for the lack of oxygen (i.e., no reducing agents were used). Second, the uptake of Hg(II) under anaerobic conditions was not proportional to the abundance of lipophilic Hg species but was dependent on the total concentration of Hg in the samples. Third, at trace Hg(II) concentrations and under anaerobic conditions, Hg(II) uptake was enhanced by the addition of yeast extract and a variety of low molecular weight organic acids. In addition to demonstrating that Hg(II) uptake by these bacteria had the characteristics of facilitated transport, these lines of evidence also support the conclusion that processes under regulatory control of the cell affected Hg(II) uptake. If these findings apply to other bacteria as well, they mean that current models of Hg(II) uptake by microorganisms in aquatic systems, which are based solely on the role of lipophilic Hg species and passive diffusion, will need to be reconsidered.Mercury undergoes a wide variety of chemical and biological transformations in aquatic ecosystems. Microorganisms play a major role in carrying out the biological transformations, many of which affect the toxicity of mercury. For example, bacteria that contain the mer operon can reduce Hg(II) to the volatile and relatively nontoxic Hg 0 (Hobman and Brown 1997). Hg(II) is transformed by bacteria in anaerobic sediments to monomethyl mercury (Compeau and Bartha 1984), which is a more toxic form of Hg(II) and is more readily bioaccumulated in the aquatic food web than inorganic mercury (Mason et al. 1996;Watras et al. 1998). For these reactions to occur, Hg(II) must first enter the cell; Acknowledgments We thank Dr. Cindy Gilmour for instruction and use of the anaerobic techniques, Dr. Bob Flett for Hg standards and analyses, and Dr. Feiyue Wang for help and discussions on metal speciation. Tamiko Hisanaga, Morris Holoka, Paul Humenchuk, Jack Switala, and Karen Scott kindly provided advice in methods development.

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“…Bienvenue et al, 1984), this low to moderate lipophilicity combined with affinity for sulfhydryl groups in proteins enhances the retention of MeHg in biological tissues (Saouter et al 1993). Therefore, the present results can be influenced according to the lipidic and proteic composition and content of the matrixes.…”

Section: Discussionmentioning

confidence: 99%

Influence of Delipidation on Hg Analyses in Biological Tissues: A Case Study for an Antarctic Ecosystem

Cipro

Bustamante

Montone

2017

Water Air Soil Pollut

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The use of low-polarity organic solvents is widespread in cleanup/extraction processes in order to carry compounds of interest, remove interferences and separate phases, among other uses. A large number of studies have used delipidation to remove excess of lipids to analyse carbon stable isotopes in biological tissues for trophic and behavioural ecology investigations. In this context, the primary aim of this study is to assess the influence of one delipidation process on the results of total mercury (Hg) analyses and the possible use of delipidated samples from previous analyses, such as for stable isotopes, in Hg level determination. Samples of vegetation (angiosperm, lichens and mosses), invertebrates (krill and limpets), fish (marbled and black rockcod), bird liver and eggs (Antarctic, Gentoo and Adélie penguins, kelp gull, Antarctic tern, cape petrel and giant southern petrel) and pinniped tissues (Weddell seal, crabeater seal, southern elephant seal and Antarctic fur seal) were analysed for Hg before and after delipidation by cyclohexane. The difference between the two measurements ranged individually from −63 to 136% (in the moss Sanionia uncinata) and the averages ranged from −60 to 66% (in pinniped tissues). The proportion of organic Hg, which presents considerable lipophilicity, but also high affinity for sulfhydryl groups in proteins, might be responsible for such variability. Given the limitations of our study, we think it is safe to say that delipidated samples could not be used to infer total Hg values in non-delipidated ones.

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Transport of mercury compounds across bimolecular lipid membranes: Effect of lipid composition, pH and chloride concentration

Cited by 87 publications

References 12 publications

Chemical speciation and toxicity of metals assessed by three bioluminescence‐based assays using marine organisms

Chemical speciation and toxicity of metals assessed by three bioluminescence‐based assays using marine organisms

Evidence for facilitated uptake of Hg(II) by Vibrio anguillarum and Escherichia coli under anaerobic and aerobic conditions

Influence of Delipidation on Hg Analyses in Biological Tissues: A Case Study for an Antarctic Ecosystem

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