Chromophoric dissolved organic matter (CDOM) was surveyed along the 160uW transect from the equatorial to the subarctic Pacific. CDOM characteristics were evaluated through the measurement of fluorescence intensity at 320 nm excitation and 420 nm emission and of absorption coefficient (a) at 320 nm, both indicative of marine humic-like CDOM. In the surface layer (,200 m), different levels but similar optical characteristics of CDOM were found among the oceanic regions studied. In the mesopelagic (200-1000 m) and abyssal layers (1000 mbottom), levels of fluorescence intensities were linearly correlated to those of absorption coefficients, with a similar slope between these layers. However, the intercept of the linear relationship between the two optical parameters was significantly lower for the mesopelagic layer than for the abyssal layer. Differences between intercepts were consistent with the transport of optically distinct CDOM to the mesopelagic layer through the formation of the North Pacific intermediate water (NPIW). At wavelengths shorter than 300 nm, the absorption coefficients of CDOM in the surface layer systematically deviated from the natural logarithmic relationships between absorption coefficients and wavelengths in the 320-350 nm range. This class of CDOM, estimated using absorption coefficient at 275 nm, was defined as surface-specific CDOM and may be derived from biocomponents. Surface-specific CDOM was higher in the relatively young mode water than in old NPIW, suggesting that this class of CDOM is semi-labile.Dissolved organic matter (DOM) in seawater comprises one of the largest reduced carbon pools on the earth's surface (Hedges and Keil 1995). The temporal and spatial variation in the concentration of dissolved organic carbon (DOC) has been investigated, and it has been reported that a substantial amount of DOC is exported from the surface to deeper layers (Doval and Hansell 2000;Hansell et al. 2002). Such findings illustrate the important dynamics of the DOM pool in the global carbon cycle. A comprehensive view of DOM temporal and spatial variability at the molecular level is essential to put DOM dynamics into a global perspective. However, a complete characterization of DOM quality is not possible with current methodologies.The optical characterization of DOM is known to be a high-throughput method, and therefore is able to determine the temporal and spatial variability of chromophoric DOM (CDOM). CDOM has recently been studied in the open ocean in connection with its role in the oceanic ecosystem as well as the biogeochemical cycle, e.g., as a factor determining light penetration under water (Nelson et al. 1998;Siegel et al. 2002) and as the substrate for formation of photoproducts (Mopper et al. 1991). In coastal environments, CDOM is derived mainly from terrestrial inputs Jaffé et al. 2004;Yamashita et al. 2008), whereas oceanic CDOM has been considered to be autochthonous and has its own processes of production and degradation . Based on field observations (Chen and Bada 199...