Transport of <scp>P</scp>ru p 3 across gastrointestinal epithelium – an essential step towards the induction of food allergy?

Tordesillas, Leticia; Gómez‐Casado, Cristina; Garrido‐Arandia, María; Murua‐García, Amaya; Palacín, Arantxa; Varela, Javier; Konieczna, Patrycja; Cuesta‐Herranz, Javier; Akdiş, Cezmi A.; O’Mahony, Liam; Díaz‐Perales, Araceli

doi:10.1111/cea.12202

Cited by 60 publications

(69 citation statements)

References 60 publications

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“…For example, the formation of lipid rafts and caveolas that allow endocytosis in epithelial cells of Prup p3, an nSLTP from peach. In addition, the reduction of Th2 cytokines of hypoallergenic peach nSLTP, compared to Prup p3 is associated with a lower transport capacity of the former (77). Der p2, may be internalized by the human airway epithelium and potentiate the airway inflammation because of its similar structure and function with the myeloid 2 differentiation protein (MD2), a TLR receptor signaling protein (78).…”

Section: Lipids May Influence the Interactions Between Pot Ential Allmentioning

confidence: 99%

The Role of Lipids in Development of Allergic Responses

Moral

Martı́nez-Naves

2017

Immune Netw

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Most allergic diseases are caused by activation of Th2 type immune responses resulting in the production of specific IgE against proteins found in normally harmless substances such as pollen, mites, epithelia or food. Allergenic substances are composed, in addition to proteins, of other compounds such as carbohydrates and lipids. Those lipids are able to promote the development of Th2-type responses associated with allergy. There are lipids found in pollen, milk or insect venom that are specifically recognized by CD1 restricted unconventional T lymphocytes, which can promote allergic reactions. Furthermore, a large number of allergens are proteins containing hydrophobic parts that specifically bind lipids that are capable to favor allergenic immune responses. Also, lipids associated to substances like pollen, dander, epithelia or the bacteria can act on cells of the innate system, including dendritic cells, which in turn lead to the differentiation of Th2-type clones. Finally, lipids may also influence the ability of allergens to be exposed to the immune system within the oral, respiratory or intestinal mucosa where allergic response occurs with great frequency.

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Section: Lipids May Influence the Interactions Between Pot Ential Allmentioning

confidence: 99%

The Role of Lipids in Development of Allergic Responses

Moral

Martı́nez-Naves

2017

Immune Netw

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“…It has been speculated that the antimicrobial activity of nsLTPs could result from the interaction and permeabilization of biological membranes 66 . Tordesillas et al 14 showed that the nsLTP from peach, Pru p 3, was internalized by Caco 2 cells through an endocytic pathway involving lipid rafts and caveolar endocytosis. The authors further reported that the lower transport rate of a hypoallergenic peach nsLTP was associated with a significantly lower expression of T H 2-related cytokines compared with Pru p 3 (Table I).…”

Section: Interaction Of Lipids and Food Allergensmentioning

confidence: 99%

Do lipids influence the allergic sensitization process?

Bublin

Eiwegger

Breiteneder

2014

Journal of Allergy and Clinical Immunology

130

111

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Allergic sensitization is a multifactorial process that is not only influenced by the allergen and its biological function per se but also by other small molecular compounds, such as lipids, that are directly bound as ligands by the allergen or are present in the allergen source. Several members of major allergen families bind lipid ligands through hydrophobic cavities or electrostatic or hydrophobic interactions. These allergens include certain seed storage proteins, Bet v 1–like and nonspecific lipid transfer proteins from pollens and fruits, certain inhalant allergens from house dust mites and cockroaches, and lipocalins. Lipids from the pollen coat and furry animals and the so-called pollen-associated lipid mediators are codelivered with the allergens and can modulate the immune responses of predisposed subjects by interacting with the innate immune system and invariant natural killer T cells. In addition, lipids originating from bacterial members of the pollen microbiome contribute to the outcome of the sensitization process. Dietary lipids act as adjuvants and might skew the immune response toward a TH2-dominated phenotype. In addition, the association with lipids protects food allergens from gastrointestinal degradation and facilitates their uptake by intestinal cells. These findings will have a major influence on how allergic sensitization will be viewed and studied in the future.

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“…Total RNA was extracted from the tissues following the method published (Tordesillas et al 2013). Gel electrophoresis (1% agarose) and spectrophotometry (260/280 nm) were used to determine RNA quality and concentration.…”

Section: Real-time Pcr In Flower and Fruit Tissuesmentioning

confidence: 99%

“…Finally, 10 ng of cDNA per well were added, and the final concentration of the primers was 1.5 uM. The amount of Pru p 3, STR, and S4D mRNA expression was normalized using the endogenous control 18S, and relative quantification was performed using the comparative threshold cycle method (2-AACt), as previously described (Tordesillas et al 2013). Changes in gene expression were calculated with reference to anthesis (7 dpa).…”

Section: Real-time Pcr In Flower and Fruit Tissuesmentioning

confidence: 99%

Identification of the ligand of Pru p 3, a peach LTP

et al. 2017

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Key message Pru p 3, a peach LTP, is located in pollinated flower styles and secreting downy hairs, transporting a derivative of camptothecin bound to phytosphingosine. Pru p 3 may inhibit a second pollination and may keep away herbivores until seed maturation. Abstract The allergen Pru p 3, a peach lipid transfer protein, has been well studied. However, its physiological function remains to be elucidated. Our results showed that Pru p 3 usually carries a lipid ligand that play an essential role in its function in plants. Using ESI-qToF, we observed that the ligand was a derivative of camptothecin binding to phytosphingosine, wich that is inserted into the hydrophobic tunnel of the protein. In addition, the described ligand displayed topoisomerase I activity inhibition and self-fluorescence, both recognized as camptothecin properties. During flower development, the highest expression of Pru p 3 was detected in the styles of pollinated flowers, in contrast to its non-expression in unpollinated pistils, where expression decreased after anthesis. During ripening, the expression of Pru p 3 were observed mainly in peel but not in pulp. In this sense, Pru p 3 protein was also localized in trichomes covering the fruit epidermis.

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Transport of Pru p 3 across gastrointestinal epithelium – an essential step towards the induction of food allergy?

Abstract: The capacity of food allergens to cross the intestinal monolayer could explain their high allergenic capacity and its fast diffusion through the body associating to severe symptoms.

Cited by 60 publications

References 60 publications

The Role of Lipids in Development of Allergic Responses

The Role of Lipids in Development of Allergic Responses

Do lipids influence the allergic sensitization process?

Identification of the ligand of Pru p 3, a peach LTP

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