Transport of soybean protein-derived antihypertensive peptide LSW across Caco-2 monolayers

Lin, Qinlu; Xu, Qingbiao; Bai, Jie; Wu, Wei; Hong, Hui; Wu, Jianping

doi:10.1016/j.jff.2017.10.011

Cited by 46 publications

(31 citation statements)

References 42 publications

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“…In this study, the TEER values of the epithelial monolayer significantly decreased when CS/PEP‐NPs incubated with Caco‐2 cells ( p < 0.05), indicating that CS/PEP‐NPs enhance cellular uptake of peptide via the tight junctions mediated paracellular route. These results were consistent with corn gluten‐derived bioactive peptides YFCLT and GLLLPH (Ding, Wang, Zhang, Yu, & Liu, ).and soybean protein‐derived LSW (Lin et al, ). In addition, it is reported that basic, positively charged complex can improve the permeability of bioactive peptides (Yamaguchi, Ito, Kurogi‐Hirayama, & Ohtsuki, ).…”

Section: Resultsmentioning

confidence: 99%

Chitosan/sodium tripolyphosphate nanoparticles as efficient vehicles for enhancing the cellular uptake of fish-derived peptide

Zhao

et al. 2018

J Food Biochem

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Methodology to enhance the intestinal absorption of peptides is an important challenge due to their easily degradation and poor permeability across the intestinal epithelium. In this study, the fish‐derived peptide (DGDDGEAGKIG)‐loaded chitosan (CS) nanoparticles (CS/PEP‐NPs) were prepared and investigated in Caco‐2 monolayer model. The results indicated zeta potential of CS/PEP‐NPs increased with the increase in molecular weight of CS (10–50 kDa). Transmission electron microscopy images revealed the CS/PEP‐NPs were uniform spherical‐shaped nanoparticles with a diameter of 50–200 nm (150 kDa). Compared to other CS/PEP‐NPs, 150‐kDa CS/PEP‐NPs performed an outstanding apparent permeability coefficient (Papp, 2.29 × 10−5 cm s−1) and cumulative amount of peptide (120 min, 2,987 ng) in Caco‐2 cells. CS/PEP‐NPs could reduce the tight junction integrity of Caco‐2 cells and enhance the intracellular fluorescence intensities of fluorescein isothiocyanate‐labeled peptide. These findings suggest that chitosan nanoparticles are promising carriers to promote intestinal absorption of fish‐derived peptide via paracellular pathway mediated by tight junctions. Practical applications Chitosans are promising carriers to promote intestinal absorption of fish‐derived peptide. The 150‐kDa CS/PEP‐NPs performed an outstanding apparent permeability coefficient (Papp, 2.29 × 10−5 cm s−1) and cumulative amount of peptide (120 min, 2,987 ng) in Caco‐2 cells. CS/PEP‐NPs could reduce the tight junction integrity of Caco‐2 cells and enhance the peptide uptake by paracellular pathway. Chitosan nanoparticles can be developed as vehicles for enhancing the cellular uptake of peptide in food industry.

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Section: Resultsmentioning

confidence: 99%

Chitosan/sodium tripolyphosphate nanoparticles as efficient vehicles for enhancing the cellular uptake of fish-derived peptide

Zhao

et al. 2018

J Food Biochem

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“…Egg ovotransferrin‐derived antihypertensive peptide Ile‐Arg‐Trp could be transported across Caco‐2 monolayers via paracellular route and PepT1 (Bejjani & Wu, ). Soybean protein‐derived antihypertensive peptide Leu‐Ser‐Trp was transported intact across Caco‐2 monolayers by paracellular diffusion and PepT1 pathways, but not by transcytosis pathway (Lin et al ., ). In another study of the transport of rapeseed protein‐derived antihypertensive peptides Leu‐Tyr, Arg‐Ala‐Leu‐Pro and Thr‐Phe, it was found that dipeptides could be transported more easily to control hypertension (Yang et al ., ).…”

Section: Transport Mechanism Of Dbps Across the Intestinal Brush‐bordmentioning

confidence: 97%

“…To study the role of PepT1 in peptide transport, small interfering RNA-mediated depletion of PepT1 has been used (Lin et al, 2017;Xu et al, 2017). In addition, sodium azide, an ATP synthesis inhibitor, could be used to inhibit the activity of PepT1 (Shimizu et al, 1997;Ding et al, 2018).…”

Section: Pept1-mediated Routementioning

confidence: 99%

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Current understanding of transport and bioavailability of bioactive peptides derived from dairy proteins: a review

Yan

Zhang

et al. 2018

Int J of Food Sci Tech

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Summary Dairy protein‐derived bioactive peptides (DBPs) have potential benefits for human health. However, their transport mechanisms and bioavailability from intestinal lumen to bloodstream are not well understood. This review summarises current understanding of their transport mechanisms across the intestinal membrane (peptide transport 1, paracellular route, transcytosis and passive transcellular diffusion) and the bioavailability of DBPs in animal and human studies. Some DBPs can escape the degradation of peptidase and reach the bloodstream at concentrations of micromolar range, and keep intact for several minutes to hours. The presences of brush‐border peptidases at the site of absorption and other peptidases in the blood, along with the peptide properties, such as molecular size and weight, stability to proteases, hydrophobicity and charge, determine their bioavailability. Developing novel analytical tools for accurate measurement and study of the transport, metabolism, and bioavailability of DBPs in vivo are expected.

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“…The peptide transporter 1 (PepT1) can transport small peptides from the intestine into the bloodstream of humans or animals [ 83 – 85 ], particularly di- and tripeptides, and its expression in intestinal epithelial cells is increased when the intestine is suffering from inflammation [ 86 ], indicating that PepT1 is a gateway to inflammatory response [ 87 ]. Similarly, PepT1 can transport various BAPs into intestinal epithelial cells to exert bioactivities [ 3 , 81 ], such as IPAV [ 23 ], KPV [ 66 ], LKP, IQW [ 88 ], LSW [ 89 ], IWH, IW [ 90 ], and VPY [ 33 ].…”

Section: Peptide Transporter Pept1mentioning

confidence: 99%

The Potential of Food Protein-Derived Bioactive Peptides against Chronic Intestinal Inflammation

Zhu

Ren

Zhang

et al. 2020

Mediators of Inflammation

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Inflammation can cause various chronic diseases like inflammatory bowel diseases. Various food protein-derived bioactive peptides (BAPs) with anti-inflammatory activity have the potential to manage these diseases. The aim of this paper is to overview the mechanisms and the molecular targets of BAPs to exert anti-inflammatory activity. In this review, the in vitro and in vivo effects of BAPs on intestinal inflammation are highlighted. The mechanism, pathways, and future perspectives of BAPs as the potential sources of therapeutic treatments to alleviate intestinal inflammation are provided, including nuclear factor-κB, mitogen-activated protein kinase, Janus kinase-signal transducer and activator of transcription, and peptide transporter 1 (PepT1), finding that PepT1 and gut microbiota are the promising targets for BAPs to alleviate the intestinal inflammation. This review provides a comprehensive understanding of the role of dietary BAPs in attenuating inflammation and gives a novel direction in nutraceuticals for people or animals with intestinal inflammation.

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Transport of soybean protein-derived antihypertensive peptide LSW across Caco-2 monolayers

Cited by 46 publications

References 42 publications

Chitosan/sodium tripolyphosphate nanoparticles as efficient vehicles for enhancing the cellular uptake of fish-derived peptide

Chitosan/sodium tripolyphosphate nanoparticles as efficient vehicles for enhancing the cellular uptake of fish-derived peptide

Current understanding of transport and bioavailability of bioactive peptides derived from dairy proteins: a review

The Potential of Food Protein-Derived Bioactive Peptides against Chronic Intestinal Inflammation

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