Transposition burst of mariner-like elements in the sequenced genome of Rhodnius prolixus

Fernández-Medina, Rita D; Granzotto, Adriana; Ribeiro, José M. C.; Carareto, Claudia M. A.

doi:10.1016/j.ibmb.2015.09.003

Cited by 12 publications

(13 citation statements)

References 66 publications

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“…1 A and C and SI Appendix, Tables A1 and A2). Many of these sequences have full-length transposases and ORFs suggesting a recent period of increased transposition (8).…”

mentioning

confidence: 99%

Genome of Rhodnius prolixus , an insect vector of Chagas disease, reveals unique adaptations to hematophagy and parasite infection

Mesquita

Vionette-Amaral

Lowenberger

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

326

418

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Rhodnius prolixus not only has served as a model organism for the study of insect physiology, but also is a major vector of Chagas disease, an illness that affects approximately seven million people worldwide. We sequenced the genome of R. prolixus, generated assembled sequences covering 95% of the genome (∼702 Mb), including 15,456 putative protein-coding genes, and completed comprehensive genomic analyses of this obligate blood-feeding insect. Although immune-deficiency (IMD)-mediated immune responses were observed, R. prolixus putatively lacks key components of the IMD pathway, suggesting a reorganization of the canonical immune signaling network. Although both Toll and IMD effectors controlled intestinal microbiota, neither affected Trypanosoma cruzi, the causal agent of Chagas disease, implying the existence of evasion or tolerance mechanisms. R. prolixus has experienced an extensive loss of selenoprotein genes, with its repertoire reduced to only two proteins, one of which is a selenocysteine-based glutathione peroxidase, the first found in insects. The genome contained actively transcribed, horizontally transferred genes from Wolbachia sp., which showed evidence of codon use evolution toward the insect use pattern. Comparative protein analyses revealed many lineage-specific expansions and putative gene absences in R. prolixus, including tandem expansions of genes related to chemoreception, feeding, and digestion that possibly contributed to the evolution of a blood-feeding lifestyle. The genome assembly and these associated analyses provide critical information on the physiology and evolution of this important vector species and should be instrumental for the development of innovative disease control methods.

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“…1 A and C and SI Appendix, Tables A1 and A2). Many of these sequences have full-length transposases and ORFs suggesting a recent period of increased transposition (8).…”

mentioning

confidence: 99%

Genome of Rhodnius prolixus , an insect vector of Chagas disease, reveals unique adaptations to hematophagy and parasite infection

Mesquita

Vionette-Amaral

Lowenberger

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

326

418

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“…For other repeat sequences as TEs (including LTR, non-LTR and class II) the total amount is very similar, being the 6% in R . prolixus genome [39,40] and 5–7% in T . infestans .…”

Section: Discussionmentioning

confidence: 99%

Comparative repeatome analysis on Triatoma infestans Andean and Non-Andean lineages, main vector of Chagas disease

et al. 2017

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Triatoma infestans is the most important Chagas disease vector in South America. Two main evolutionary lineages, named Andean and non-Andean, have been recognized by geographical distribution, phenetic and genetic characteristics. One of the main differences is the genomic size, varying over 30% in their haploid DNA content. Here we realize a genome wide analysis to compare the repetitive genome fraction (repeatome) between both lineages in order to identify the main repetitive DNA changes occurred during T. infestans differentiation process. RepeatExplorer analysis using Illumina reads showed that both lineages exhibit the same amount of non-repeat sequences, and that satellite DNA is by far the major component of repetitive DNA and the main responsible for the genome size differentiation between both lineages. We characterize 42 satellite DNA families, which are virtually all present in both lineages but with different amount in each lineage. Furthermore, chromosomal location of satellite DNA by fluorescence in situ hybridization showed that genomic variations in T. infestans are mainly due to satellite DNA families located on the heterochromatic regions. The results also show that many satDNA families are located on the euchromatic regions of the chromosomes.

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“…Repeatomes of R. prolixus, R. montenegrensis and R. marabaensis were assembled and annotated de novo from unassembled reads in order to determine whether these closely related lineages underwent shared or independent bursts of Mariner elements than those previously reported in R. prolixus [31,32]. First, our comparative analysis of the three species' repeatomes reveals that the TE contents of Rhodnius species complexes have been largely underestimated.…”

Section: Discussionmentioning

confidence: 96%

“…This proportion can be even higher in plants, representing up to 85% of the genome in grasses [4]. Despite the documented deleterious The low genetic diversity among R. prolixus populations [24,25,33,34] led Fernandez-Medina et al to propose that the burst of transposition of the prolixus1 and prolixus2 Mariner subfamilies could have started as a result of the ecological and populational constraints of the species [32]. The authors also suggested that the continuous expansion of these TEs could be associated with the increased adoption of domestic habitats by man, which occurred with the colonization of the Americas [25].…”

Section: Introductionmentioning

confidence: 99%

“…Most of the Mariner sequences are full-length, suggesting that they are or have been active until recently. Moreover, most of the Mariner sequences are related to TEs found in seven well-established insect TE subfamilies (irritans/himar, mellifera, cecropia, mauritiana, vertumnana, and rosa) in addition to two novel Mariner subfamilies, prolixus1 and prolixus2, which exhibit a high number of copies [32]. In the prolixus1 subfamily, three clades of sequences were identified, one with more than 8,000 copies (clade I) and the two others with 846 (clade II) and 550 (clade III) genomic copies.…”

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confidence: 99%

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Homology-Free Detection of Transposable Elements Unveils Their Dynamics in Three Ecologically Distinct Rhodnius Species

Castro

Goubert

Monteiro

et al. 2020

Genes

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Transposable elements (TEs) are widely distributed repetitive sequences in the genomes across the tree of life, and represent an important source of genetic variability. Their distribution among genomes is specific to each lineage. A phenomenon associated with this feature is the sudden expansion of one or several TE families, called bursts of transposition. We previously proposed that bursts of the Mariner family (DNA transposons) contributed to the speciation of Rhodnius prolixus Stål, 1859. This hypothesis motivated us to study two additional species of the R. prolixus complex: Rhodnius montenegrensis da Rosa et al., 2012 and Rhodnius marabaensis Souza et al., 2016, together with a new, de novo annotation of the R. prolixus repeatome using unassembled short reads. Our analysis reveals that the total amount of TEs present in Rhodnius genomes (19% to 23.5%) is three to four times higher than that expected based on the original quantifications performed for the original genome description of R. prolixus. We confirm here that the repeatome of the three species is dominated by Class II elements of the superfamily Tc1-Mariner, as well as members of the LINE order (Class I). In addition to R. prolixus, we also identified a recent burst of transposition of the Mariner family in R. montenegrensis and R. marabaensis, suggesting that this phenomenon may not be exclusive to R. prolixus. Rather, we hypothesize that whilst the expansion of Mariner elements may have contributed to the diversification of the R. prolixus-R. robustus species complex, the distinct ecological characteristics of these new species did not drive the general evolutionary trajectories of these TEs.

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Transposition burst of mariner-like elements in the sequenced genome of Rhodnius prolixus

Cited by 12 publications

References 66 publications

Genome of Rhodnius prolixus , an insect vector of Chagas disease, reveals unique adaptations to hematophagy and parasite infection

Genome of Rhodnius prolixus , an insect vector of Chagas disease, reveals unique adaptations to hematophagy and parasite infection

Comparative repeatome analysis on Triatoma infestans Andean and Non-Andean lineages, main vector of Chagas disease

Homology-Free Detection of Transposable Elements Unveils Their Dynamics in Three Ecologically Distinct Rhodnius Species

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