In order to study the influence of geometric nonlinearity and material parameters on the free vibration behavior of the cylindrical skeleton supported prestressed fabric composite membrane. In this paper, based on von Karman's large deflection theory and D’Alembert's principle, the governing equations of nonlinear viscous damped prestressed free vibration of frame supported anisotropic membrane structures were established. By using Galerkin and KBM perturbation method, the analytical expressions of frequency function, displacement function and mode shape of nonlinear free vibration were obtained. In order to verify the effectiveness and effective range of the method, the fourth order Runge-Kutta method was used for numerical calculation. The calculation examples of membrane material parameters were given. The calculation and analysis of different membrane prestress, different length width ratio, different rise span ratio and different material parameters (elastic modulus ratio, viscous damping and material density) were carried out. The results were compared with the numerical results obtained by Runge-Kutta method to verify the accuracy of the method. The research results of this paper provide a theoretical reference for the selection of membrane materials, the subsequent calculation of wind-induced stability of steel skeleton membrane structure and the design of wind-driven rain resistance.