Traumatic Tympanic Membrane Perforations Diagnosed in Emergency Departments

Carniol, Eric T.; Bresler, Amishav Y.; Shaigany, Kevin; Svider, Peter F.; Baredes, Soly; Eloy, Jean Anderson; Yue, Ying

doi:10.1001/jamaoto.2017.2550

Cited by 25 publications

(37 citation statements)

References 18 publications

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“…Mechanical Characterization: A macroindentation approach was developed for evaluating the mechanical response of the fabricated test cases. Inspired by the TM perforations caused by foreign body instrumentation, such as from cotton buds or other sharp objects, [20] a cylindrical stainless steel probe with a diameter of 2.5 mm was used to evaluate the stress-strain profile of the developed scaffolds until the point of rupture (Figure 5A). The outer perimeter of the fabricated scaffolds was sealed onto the rim of a hollow tube (inner diameter = 8 mm, outer diameter = 12 mm), using a double-sided tape (Van Loenen Instruments, the Netherlands).…”

Section: Methodsmentioning

confidence: 99%

“…The 3D patterns chosen for this study were first analyzed computationally, following which the PEOT/PBT-based TM scaffolds were biofabricated and characterized with respect to their mechanical, acoustical, and biological response (Figure 1). A macroindentation approach, inspired from one of the leading causes of traumatic TM perforations -foreign body instrumentation, [20] was implemented to evaluate the mechanical contribution of the varying fiber arrangements, whereas, the acoustical characterization was conducted using LDV. Biological characterization of the fabricated scaffolds was performed with human dermal fibroblasts, neonatal (NHDFs), and human mesenchymal stromal cells (hM-SCs).…”

Section: Introductionmentioning

confidence: 99%

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Mimicking the Human Tympanic Membrane: The Significance of Scaffold Geometry

Anand

Stoppe

Lucena

et al. 2021

Adv Healthcare Materials

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The human tympanic membrane (TM) captures sound waves from the environment and transforms them into mechanical motion. The successful transmission of these acoustic vibrations is attributed to the unique architecture of the TM. However, a limited knowledge is available on the contribution of its discrete anatomical features, which is important for fabricating functional TM replacements. This work synergizes theoretical and experimental approaches toward understanding the significance of geometry in tissue-engineered TM scaffolds. Three test designs along with a plain control are chosen to decouple some of the dominant structural elements, such as the radial and circumferential alignment of the collagen fibrils. In silico models suggest a geometrical dependency of their mechanical and acoustical responses, where the presence of radially aligned fibers is observed to have a more prominent effect compared to their circumferential counterparts. Following which, a hybrid fabrication strategy combining electrospinning and additive manufacturing has been optimized to manufacture biomimetic scaffolds within the dimensions of the native TM. The experimental characterizations conducted using macroindentation and laser Doppler vibrometry corroborate the computational findings. Finally, biological studies with human dermal fibroblasts and human mesenchymal stromal cells reveal a favorable influence of scaffold hierarchy on cellular alignment and subsequent collagen deposition.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Mimicking the Human Tympanic Membrane: The Significance of Scaffold Geometry

Anand

Stoppe

Lucena

et al. 2021

Adv Healthcare Materials

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“…Aural foreign bodies in human beings are treated frequently in emergency settings, with visits reported to exceed 50 000 in one year 4. Cotton‐tipped applicators are the most common item removed from the ears of adults, while jewellery products are most common in young children 4 5. Reports of aural foreign bodies in dogs are far fewer, with the majority of cases caused by grass seeds 3.…”

Section: Discussionmentioning

confidence: 99%

Unilateral otitis externa induced by a plastic foreign body

May

2018

Vet Record Case Reports

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A nine-year-old, male, neutered Tibetan terrier was evaluated for unilateral otitis externa associated with the history of a dog bite injury to the pinna five years prior. Examination findings of the left ear included severe inflammation, abundant purulent exudate and a polypoid mass, obscuring view of the horizontal ear canal and tympanic membrane. CT scan confirmed the visualised mass location and confinement to the ear canal. Aerobic otic culture revealed abundant colonies of Staphylococcus pseudintermedius and Corynebacterium species. Histopathology was consistent with a fibrous polyp. Otitis externa treatment provided minimal relief following mass removal, and the patient was re-examined. A transparent plastic foreign body lodged within the horizontal canal was identified and removed. The item was recognised as a cap used for packaging of topical treatments prescribed at the time of the original traumatic injury. All clinical signs resolved following removal of the foreign body and oral anti-inflammatory therapy.

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“…A macro-indentation approach was developed for evaluating the mechanical response of the fabricated test cases. Inspired by the TM perforations caused by foreign body instrumentation, such as from cotton buds or other sharp objects [40], a cylindrical stainless steel probe with a diameter of 2.5 mm was used to evaluate the stress-strain profile of the developed scaffolds until the point of rupture ( Figure 4A ). The outer perimeter of the fabricated scaffolds were fixed onto the rim of a hollow tube (inner diameter = 8 mm, outer diameter = 12 mm), using a double-sided tape (Van Loenen Instruments, the Netherlands).…”

Section: Methodsmentioning

confidence: 99%

“…The 3D patterns chosen for this study were first analyzed computationally, following which the PEOT/PBT based TM scaffolds were biofabricated and characterized with respect to their mechanical, acoustical and biological response. A macro-indentation approach, inspired from one of the leading causes of traumatic TM perforations – foreign body instrumentation [40], was implemented to evaluate the mechanical contribution of the varying fiber arrangements, whereas, the acoustical characterization was conducted using LDV. Biological characterization of the fabricated scaffolds were performed with human dermal fibroblasts (hDFs) and human mesenchymal stromal cells (hMSCs).…”

Section: Introductionmentioning

confidence: 99%

Mimicking the Human Tympanic Membrane: the Significance of Geometry

Anand

Stoppe

Lucena

et al. 2020

Preprint

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The human tympanic membrane (TM) captures sound waves reaching the outer ear from the environment and transforms them into mechanical motion. The successful transmission of these acoustic vibrations in varying frequency ranges is attributed to the structural architecture of the TM. However, a limited knowledge is available on the contribution of its discrete anatomical features, which is important to fabricate functional biomimetic TM replacements. This work synergizes theoretical and experimental approaches toward understanding the significance of geometry in tissue engineered TM scaffolds. Three test designs along with a plain control were chosen to decouple some of the dominant structural attributes, such as, the radial and circumferential alignment of the collagen fibrils. In silico models suggested a geometrical dependency of their mechanical and acoustical responses, where the presence of radially aligned fibers was observed to have a more prominent effect compared to their circumferential counterparts. Following which, a hybrid fabrication strategy combining electrospinning and additive manufacturing was optimized to manufacture hierarchical scaffolds within the dimensions of the native TM. The experimental characterizations conducted using macro-indentation and laser Doppler vibrometry were in line with the computational models. Finally, biological studies performed with human dermal fibroblasts and human mesenchymal stromal cells, revealed a favorable influence of scaffold hierarchy on cellular alignment and subsequent collagen deposition.Abstract FigureGraphical abstract.Schematic diagram illustrating the overall flowchart of the work. 3D: three-dimensional; ES: electrospinning; FDM: fused deposition modeling; TM: tympanic membrane.

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Traumatic Tympanic Membrane Perforations Diagnosed in Emergency Departments

Cited by 25 publications

References 18 publications

Mimicking the Human Tympanic Membrane: The Significance of Scaffold Geometry

Mimicking the Human Tympanic Membrane: The Significance of Scaffold Geometry

Unilateral otitis externa induced by a plastic foreign body

Mimicking the Human Tympanic Membrane: the Significance of Geometry

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