Treadmill exercise influences the microRNA profiles in the bone tissues of mice

Yang, Huan; Cao, Zhen; Wang, Yang; Wang, Jiahui; Gao, Jintao; Han, Baohui; Yu, Fangmei; Qin, Yixiong; Guo, Yong

doi:10.3892/etm.2021.10467

Cited by 5 publications

(6 citation statements)

References 68 publications

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“…BSP was suggested to be the early marker for osteogenic differentiation of stem cells [44]. The OCN gene encodes protein secreted by osteoblasts that regulates bone remodeling [45]. COL1A1 is also known as an osteogenic marker, and the impairment in collagen formation due to mutation of COL1A1 may lead to fragility of bone [46].…”

Section: Discussionmentioning

confidence: 99%

Vitamin D Enhanced the Osteogenic Differentiation of Cell Spheroids Composed of Bone Marrow Stem Cells

et al. 2021

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Background and Objectives: Vitamin D is a bone modulator widely used in regenerative medicine. This study aimed to analyze the effects of vitamin D on the osteogenic differentiation and mineralization of human mesenchymal stem cells. Materials and Methods: Spheroids were fabricated using human bone marrow-derived stem cells, and were cultured in the presence of vitamin D at concentrations of 0, 0.1, 1, 10, and 100 nM. Stem cell spheroids were fabricated and the morphological evaluation was conducted on days 1, 3, 7 and 14. Determination of qualitative cellular viability was performed with Live/Dead Kit assay on days 1 and 7. Quantitative cellular viability was evaluated with Cell Counting Kit-8 on days 1, 3, 7, and 14. To analyze the osteogenic differentiation of cell spheroids, alkaline phosphatase activity assays were performed with commercially available kit on days 7 and 14. Real-time polymerase chain reaction was used to determine the expression levels of RUNX2, BSP, OCN, and COL1A1 on days 7 and 14. Results: The stem cells produced well-formed spheroids, and addition of vitamin D did not result in any noticeable changes in the shape. The addition of vitamin D did not significantly change the diameter of the spheroids at 0, 0.1, 1, 10, or 100 nM concentrations. Quantitative cell viability results from days 1, 3, 7 and 14 showed no significant difference between groups (p > 0.05). There was significantly higher alkaline phosphatase activity in the 0.1 nM group when compared with the control group on day 14 (p < 0.05). Real-time polymerase chain reaction results demonstrated that the mRNA expression levels of RUNX2, OCN, and COL1A1 were significantly increased when vitamin D was added to the culture. Conclusions: Based on these findings, we concluded that vitamin D could be applied to the increased osteogenicity of stem cell spheroids.

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Section: Discussionmentioning

confidence: 99%

Vitamin D Enhanced the Osteogenic Differentiation of Cell Spheroids Composed of Bone Marrow Stem Cells

et al. 2021

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“…The potential upstream mediators of osteogenesis and neurogenesis in mice femora were assessed. Previous literature explored the effects of 8-week treadmill exercise training (13 m/min; 9° slope; for 40 min/day; 6 days/week) on the miRNA expression in the femora of 4-month-old male BALB/c mice ( Yang et al, 2021 ). Re-examination of the microarray dataset (GSE179201) shown in Figure 4A revealed that a total of 191 out of 1,900 miRNAs were differentially expressed ( p < 0.05) between the non-exercise and aerobic exercise training groups.…”

Section: Resultsmentioning

confidence: 99%

Trabecular Bone Microarchitecture Improvement Is Associated With Skeletal Nerve Increase Following Aerobic Exercise Training in Middle-Aged Mice

et al. 2022

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Advancing age is associated with bone loss and an increased risk of osteoporosis. Exercise training improves bone metabolism and peripheral nerve regeneration, and may play a critical role in osteogenesis and increase in skeletal nerve fiber density. In this study, the potential positive role of aerobic exercise training in bone metabolism and skeletal nerve regeneration was comprehensively evaluated in 14-month-old male C57BL/6 mice. The mice were divided into two groups: no exercise (non-exercise group) and 8-weeks of aerobic exercise training (exercise group), with six mice in each group. Dual-energy X-ray absorptiometry and micro-computed tomography showed that femoral and tibial bone parameters improved after aerobic exercise training. Greater skeletal nerve fiber density was also observed in the distal femoral and proximal tibial periostea, measured and analyzed by immunofluorescence staining and confocal microscopy. Pearson correlation analysis revealed a significant association between skeletal nerve densities and trabecular bone volume/total volume ratios (distal femur; R2 = 0.82, p < 0.05, proximal tibia; R2 = 0.59, p = 0.07) in the exercise group; while in the non-exercise group no significant correlation was found (distal femur; R2 = 0.10, p = 0.54, proximal tibia; R2 = 0.12, p = 0.51). Analysis of archival microarray database confirmed that aerobic exercise training changed the microRNA profiles in the mice femora. The differentially expressed microRNAs reinforce the role of aerobic exercise training in the osteogenic and neurogenic potential of femora and tibiae. In conclusion, 8-weeks of aerobic exercise training positively regulate bone metabolism, an effect that paralleled a significant increase in skeletal nerve fiber density. These findings suggest that aerobic exercise training may have dual utility, both as a direct stimulator of bone remodeling and a positive regulator of skeletal nerve regeneration.

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“…There was an increase in the expression of alkaline phosphatase and osteocalcin in the femur of rats given treadmill intervention, where these two indicators have association with the formation and mineralization of bone matrix. Treadmill exercise can increase the maturation and differentiation of bone tissue (Yang et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

Effects of Different Intensity Exercises On Bone Structure of Young Male Wistar Rats

Salim,

Goenawan,

Kastella

et al. 2023

J. pendidik. jasmani olahraga

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Many adolescents have insufficient physical activity. Regular physical activity from young age can improve health and fitness. Getting regular exercise and eating healthy diet should be encouraged among kids and teenagers to prevent various diseases in adulthood and old age, such as osteoporosis and fractures. When exercising, a prescription is needed that is tailored to each individual abilities. The intensity of exercise will be different for each individual in accordance with their age, physiological function, genetics, economic status, and previous comorbid diseases. There is no standardised exercise prescription proven to increase bone mineral density in adolescents. Based on the above description, a study was conducted on the provision of different intensity exercise and its effect on bone morphology and density. In this study, male Wistar rats, aged 15–16 weeks (body weight ± 250-350 grams; n = 20), were split into 4 groups, namely control group, low intensity exercise group, moderate intensity exercise group, and high intensity exercise group. All groups were receiving intervention for 8 weeks. Then, the rats were sacrificed and the femoral bones were isolated. Femur weight and femur length was checked. Bone density was examined using X-Ray and calculated by statistical tests. Research was conducted in the Central Laboratory, Universitas Padjadjaran. There were significant differences of femur weight between high intensity exercise group and control and low intensity exercise groups (p 0.05). Moderate and high intensity exercises significantly increased femur length as opposed to control and low intensity exercise groups (p 0.05). Bone density was higher in moderate and high intensity exercise groups (p 0.05). Moderate to high intensity exercise can increase bone health, seen from significances of bone morphology analysis and a higher density compared to low intensity exercise.

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Treadmill exercise influences the microRNA profiles in the bone tissues of mice

Cited by 5 publications

References 68 publications

Vitamin D Enhanced the Osteogenic Differentiation of Cell Spheroids Composed of Bone Marrow Stem Cells

Vitamin D Enhanced the Osteogenic Differentiation of Cell Spheroids Composed of Bone Marrow Stem Cells

Trabecular Bone Microarchitecture Improvement Is Associated With Skeletal Nerve Increase Following Aerobic Exercise Training in Middle-Aged Mice

Effects of Different Intensity Exercises On Bone Structure of Young Male Wistar Rats

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