Treating Denim Fabrics with Trichoderma Reesei Cellulases

Heikinheimo, Liisa; Büchert, Johanna; Miettinen-Oinonen, Arja; Suominen, Pirkko

doi:10.1177/004051750007001106

Cited by 91 publications

(46 citation statements)

References 9 publications

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“…Biostoning of fabric means the use of cellulases in place of, or in addition to, the use of pumice stones for the treatment of denim fabric to impart a stonewashed effect. Heikinheimo et al (7) showed that T. reesei-purified cellulase EGII was the most effective at removing color from denim, producing a good stonewashing effect with the lowest hydrolysis level. Endoglucanases are important also for degradation of ␤-glucan in feed.…”

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confidence: 99%

Enhanced Production of Trichoderma reesei Endoglucanases and Use of the New Cellulase Preparations in Producing the Stonewashed Effect on Denim Fabric

Miettinen-Oinonen¹,

Suominen²

2002

Appl Environ Microbiol

117

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number of the egl2 expression cassette. One copy of the egl2 expression cassette in which the egl2 was under the cbh1 promoter increased production of endoglucanase activity 2.3-fold, and two copies increased production about 3-fold above that of the parent strain. When the enzyme with elevated EGII content was used, an improved stonewashing effect on denim fabric was achieved. A T. reesei strain producing high amounts of EGI and -II activities without CBHI and -II was constructed by replacing the cbh2 locus with the coding region of the egl2 gene in the EGI-overproducing CBHI-negative strain. Production of endoglucanase activity by the EG-transformant strain was increased fourfold above that of the host strain. The filter paper-degrading activity of the endoglucanase-overproducing strain was lowered to below detection, presumably because of the lack of cellobiohydrolases.The filamentous fungus Trichoderma reesei is known as an efficient producer of cellulases. The cellulolytic system of T. reesei is composed of two cellobiohydrolases (CBHI and CBHII) and at least five endoglucanases (EGI, EGII, EGIII, EGIV, and EGV) (19,20). Lack of EGII (originally called EGIII [18]) production reduces the endoglucanase activity in the culture supernatant by as much as 55%, whereas lack of EGI reduces it by only 25% (21). Thus, EGII is proposed to account for most of the endoglucanase activity produced by T. reesei (21). T. reesei EGI represents 5 to 10% of the secreted protein (16). The production of EGI has been improved in T. reesei by placing the egl1 gene under the control of the strong promoter of the Trichoderma CBHI (cbh1) gene and by increasing the copy number of the egl1 gene (8).Cellulases are used widely in the textile industry in treatments of cellulose-containing textile materials during their manufacture and finishing (5). The most well-known application is the use of cellulases in biostoning. Biostoning of fabric means the use of cellulases in place of, or in addition to, the use of pumice stones for the treatment of denim fabric to impart a stonewashed effect. Heikinheimo et al. (7) showed that T. reesei-purified cellulase EGII was the most effective at removing color from denim, producing a good stonewashing effect with the lowest hydrolysis level. Endoglucanases are important also for degradation of ␤-glucan in feed. Degradation of ␤-glucan lowers the viscosity of the intestinal contents and this improves the quality of the feed (3).In this study we have constructed T. reesei strains that produce elevated amounts of endoglucanase activity. The aim of our work was to construct different tailored high endoglucanase activity-producing strains for specific applications. We have improved the production of the EGII enzyme in T. reesei and we have constructed a T. reesei strain that produces high amounts of EGI and -II without any cellobiohydrolases. Cellulase preparations derived from these T. reesei overproduction strains were tested on the biostoning application. MATERIALS AND METHODSMicrobial strains and plasmi...

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confidence: 99%

Enhanced Production of Trichoderma reesei Endoglucanases and Use of the New Cellulase Preparations in Producing the Stonewashed Effect on Denim Fabric

Miettinen-Oinonen¹,

Suominen²

2002

Appl Environ Microbiol

117

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“…Oxidative bleaching agent with or without the addition of stones have also been used to get fading effect. Difficulty in removing residual pumice from fabric, damage to equipment and clogging of machine drainage passage due to particulate material proved to be major drawbacks with the technique (Heikinheimo et al 2000). Later on use of enzymes became a sustainable option to get the worn out look in denim.…”

Section: Introductionmentioning

confidence: 99%

“…Denim fabric has been treated with Trichoderma Reesei cellulases and compared for stone washing denim fabrics (Heikinheimo et al 2000). The redeposition of indigo after treating with cellulase has been examined to certain extent .…”

Section: Introductionmentioning

confidence: 99%

Enzyme washing of indigo and sulphur dyed denim

Patra¹,

Madhu²,

Bala³

2018

Fash Text

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A wide ranging finish effects have been given to denim fabrics in industrial scale to make it more fashionable and functional. Among the numerous wet and dry treatments given, worn out looks of various kinds have received the maximum attention. In this context, herein, two denim fabrics, one dyed with vat indigo and the other with sulphur black were enzyme washed. The enzymes used were acid cellulase and neutral cellulase and the treatments were done at varying concentrations, time and mechanical agitation. The trials so taken were based on design of experiments, and the effect was analysed in terms of decrease in colour depth and weight loss. Back staining of fabric during the wash was also checked and acid enzyme despite higher colour removal, caused significant staining in sulphur dyed quality. Attempt was made to contain this back staining by suitable treatment. The application conditions of cellulases on the denim qualities were also optimized for fading effect.

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“…The optimal pH for a particular cellulase depends upon its origin (Schindler and Hauser 2004). Commercial cellulases for biopolishing originate from Trichoderma reesei and Humicola insolens (Azevedo et al 2000;Heikinheimo et al 2000). Acid cellulases are Trichodermabased products which work best at pH 4.5-5.5 at a temperature of 45-55°C (Shah 2014;Choudhury 2006).…”

Section: Introductionmentioning

confidence: 99%

Effects of biopolishing on the quality of cotton fabrics using acid and neutral cellulases

Uddin

2015

Text Cloth Sustain

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The aims of the study were to analyze the treatment conditions for the use of acid and neutral cellulase enzymes and evaluate the changes in the various properties of the treated fabrics. In this study, biopolishing was carried out on bleached fabric using acid cellulase (Mega PK) and neutral cellulase (Mega L-1009D) considering three factors: concentration (0.5, 1, and 1.5 %), temperature (45, 55, and 65°C), and time (40, 50, and 60 min). Changes in the physical properties such as weight loss, strength loss, pilling resistance, abrasion resistance (mass loss), and bending length of the biopolished fabrics were measured. Using Mega PK, weight loss and strength loss were found within 0.25 to 1.42 % and 1.7 to 24.7 %, respectively, while these values were found within 0.20 to 1.22 % and 1.5 to 18.9 %, respectively, using Mega L. On the other hand, pilling and abrasion resistance of the fabrics were improved due to biopolishing. In addition, bending length was found maximum 31.62 % lower than the untreated bleached fabric using Mega PK and 44.26 % lower using Mega L. It was also concluded that 1 % Mega PK showed better results when applied at 55°C for 40 min, whereas 1 % Mega L showed better results when applied at 55°C for 50 min among all the treatment conditions.

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Treating Denim Fabrics with Trichoderma Reesei Cellulases

Cited by 91 publications

References 9 publications

Enhanced Production of Trichoderma reesei Endoglucanases and Use of the New Cellulase Preparations in Producing the Stonewashed Effect on Denim Fabric

Enhanced Production of Trichoderma reesei Endoglucanases and Use of the New Cellulase Preparations in Producing the Stonewashed Effect on Denim Fabric

Enzyme washing of indigo and sulphur dyed denim

Effects of biopolishing on the quality of cotton fabrics using acid and neutral cellulases

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