Treatment and clarification of fermented broth in bacterial enzyme production

Abstract

Acetic acid, calcium chloride, aluminium sulphate and polyacrylamide were tried for flocculation and settling of suspended solids. Adjustment of pH, centrifugation, precoat filtration with filter aid were studied for clarification of fermentation broth in alpha-amylase production.Combination of aluminium sulphate and polyacrylamide as flocculating agents and precoat filtration was found to be the best strategy for alpha-amylase enzyme recovery from the broth.

Recovery of an alkaline proteinase from fermentation broth using flocculation for cell removal

“…Removal of the cells, solids and colloids from fermentation broth is primary operation step in the recovery of extracellular enzymes (Bautista et al, 1986;Mukhopadhyay et al, 1990) for which vacuum rotary drum filters and continuous disc centrifuges are usually used. A recent development in crossflow microfiltration has also been applied to enzyme recovery (Whittington, 1990).…”

Clarification of bacterial broth containing high ?-amylase activity

“…In this paper we report the results of treatment of broth having high cr-amylase activity with different types of coagulatingflocculating agents and subsequent clarification of broth. This paper is a part of continuing research activity in our laboratory aimed at developing a process know-how for producing amylolytic enzymes Mukhopadhyay et al, 1990).…”