Treatment of bagasse pulp with cellulase-free xylanases from an alkalophilic Bacillus sp. Sam-3

Shah, Ashit; Sidid, S.S.; Ahmad, Absar; Rele, M. V.

doi:10.1016/s0960-8524(98)00133-3

Cited by 31 publications

(11 citation statements)

References 15 publications

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“…Many Bacillus sp. are also widely reported to produce enzymes of industrial application in paper industry 53, 54, 55, 56, 57, 58, 59, 60 and bast fiber degumming 61, 62. Consequently, pretreatment with the combined Sphingomonas paucimobilis and Bacillus strains improved the hydrolysis of office paper from municipal wastes 63, justifying the observed presence of both organisms during the incubation period.…”

Section: Discussionmentioning

confidence: 96%

Changes in the bacterial community structure and diversity during bamboo retting

Mueller

Castro

et al. 2011

Biotechnology Journal

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Microbial retting is a critical step in obtaining fiber bundles from bamboo culm using indigenous microorganisms. A cultivation-independent technique for monitoring the changes in bacteria community during bamboo retting was applied in this work. This technique involves genetic profiling of PCR-amplified small-subunit rRNA and the single-strand conformation polymorphism (SSCP) gel analysis of the PCR-amplified 16S rDNA fragments. The study revealed that both the structure and the diversity of investigated communities varied with the incubation periods and sample locations. The bacteria bands from SCCP gel profiles related to Bacillus sp. decreased in intensity, and Phaeospirillum sp. and Azospirillum brasilense completely disappeared during the 4(th) and 5(th) month of incubation, while the bands related to the Sphingomonas japonica, Alphaproteobacterium Ellin335 and Microbacterium sp. increased. The bands closely related to Sphingomonads, Brevundimonas brasilense, Pseudoclavibacter sp., Agrococcus jenensis and Oxalophagus oxalicus remained dominant during the whole incubation period. This study showed that the use of PCR assay targeting 16S rRNA and SCCP profiling provided valuable information on monitoring the bacteria dynamic changes occurring in the bacteria community during bamboo retting, which is crucial for controlling the quality of the retting process and improving the retting efficiency, and thus benefits for fiber recovery.

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Section: Discussionmentioning

confidence: 96%

Changes in the bacterial community structure and diversity during bamboo retting

Mueller

Castro

et al. 2011

Biotechnology Journal

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“…() and Shah et al . (). One unit (U) of enzyme was defined as the amount of enzyme that releases 1 μmol of reducing sugar in 1 min.…”

Section: Methodsmentioning

confidence: 97%

“…The mixture was stirred well, static stood at 4°C for 1 h and clarified by centrifugation. The b-mannanase, xylanase and cellulase activity was determined using DNS method according to Khanongnuch et al (1998) and Shah et al (1999). One unit (U) of enzyme was defined as the amount of enzyme that releases 1 lmol of reducing sugar in 1 min.…”

Section: Methodsmentioning

confidence: 99%

Screening and selection of Bacillus spp. for fermented corticate soybean meal production

et al. 2012

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Aims: To screen and select the Bacillus spp. from Tua-nao of northern Thailand for fermented corticate soybean meal (FCSBM) production. Methods and Results: After isolation of Bacillus spp. from Tua-nao was carried out, cellulase, hemicellulases (i.e., b-mannanase and xylanase) and phytase production by isolated Bacillus spp. were determined. B. subtilis isolate MR10 showed the highest b-mannanase, xylanase and phytase production at 280, 41 and 16 U g À1 substrate, respectively, while the highest cellulase production was found in TK8 at 25 U g À1 substrate. FCSBMs produced by single starter and mixed starter of both isolates showed the better properties than those of corticate soybean meal (CSBM), i.e., higher in soluble sugar, protein and phosphate content, smaller sugar molecules and better digestibility and absorbability than those of CSBM. Moreover, FCSBMs had no toxicity effect on mouse fibroblast cell line (3T3) but had an inhibitory effect on lung cancer cell line (CorL23). Conclusions: B. subtilis isolate MR10 and TK8 were selected for FCSBMs production because of their role as nutritional enhancer for CSBM and their safety. Significance and Impact of the Study: The results of this study were useful for FCSBM production process that can be applied as feed ingredient for monogastric animals.

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“…One unit of xylanase activity was defined as the amount of enzyme that produced 1 lmol xylose equivalent min )1 under the assay conditions. Production of xylanases from alkaliphilic Bacillus strains Xylanase production from the alkaliphilic Bacillus strains NCL (87-6-10) and Sam III was carried out as described earlier [1,18]. The cell-free broth was concentrated by ultrafiltration using a YM-3 membrane and precipitated with ammonium sulphate (60% saturation).…”

Section: Xylanase Assaymentioning

confidence: 99%

Compatibility of alkaline xylanases from an alkaliphilic Bacillus NCL (87-6-10) with commercial detergents and proteases

Kumar

Balakrishnan

Rele

2004

Journal of Industrial Microbiology and Biotechnology

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Alkaline xylanases from alkaliphilic Bacillus strains NCL (87-6-10) and Sam III were compared with the commercial xylanases Pulpzyme HC and Biopulp for their compatibility with detergents and proteases for laundry applications. Among the four xylanases evaluated, the enzyme from the alkaliphilic Bacillus strain NCL (87-6-10) was the most compatible. The enzyme retained its full activity (40 degrees C for 1 h) in the presence of detergents, whereas Pulpzyme HC and Sam III showed only 30% and 50% of their initial activity, respectively. Biopulp, though stable to detergents, had only marginal activity (5%)at pH 10. However, all four enzymes retained significant activity (80%) for 60 min in the presence of the proteases Alcalase and Conidiobolus protease. Supplementation of the enzyme enhanced the cleaning ability of the detergents.

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Treatment of bagasse pulp with cellulase-free xylanases from an alkalophilic Bacillus sp. Sam-3

Cited by 31 publications

References 15 publications

Changes in the bacterial community structure and diversity during bamboo retting

Changes in the bacterial community structure and diversity during bamboo retting

Screening and selection of Bacillus spp. for fermented corticate soybean meal production

Compatibility of alkaline xylanases from an alkaliphilic Bacillus NCL (87-6-10) with commercial detergents and proteases

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