Treatment of Chrysanthemum Synthetic Seeds by Air SDBD Plasma

Abstract

Herein, we present the effect of surface dielectric barrier discharge (SDBD) air cold plasma on regrowth of chrysanthemum synthetic seeds (synseeds) and subsequent plantlet development. The plasma system used in this study operates in air at the frequency of 50 Hz. The detailed electrical characterization of SDBD was shown, as well as air plasma emission spectra obtained by optical emission spectroscopy. The chrysanthemum synseeds (encapsulated shoot tips) were treated in air plasma for different treatment times (0, 5 or 10 min). Plasma treatment significantly improved the regrowth and whole plantlet development of chrysanthemum synseeds under aseptic (in vitro) and non-aseptic (ex vitro) conditions. We evaluated the effect of SDBD plasma on synseed germination of four chrysanthemum cultivars after direct sowing in soil. Germination of synseeds directly sowed in soil was cultivar-dependent and 1.6−3.7 fold higher after plasma treatment in comparison with untreated synseeds. The study showed a highly effective novel strategy for direct conversion of simple monolayer alginate chrysanthemum synseeds into entire plantlets by plasma pre-conversion treatment. This treatment reduced contamination and displayed a considerable ex vitro ability to convert clonally identical chrysanthemum plants.

Dose-Dependent Effects in Plasma Oncotherapy: Critical In Vivo Immune Responses Missed by In Vitro Studies

“…In our work, OES is utilized to determine the composition of the reactive species generated during the CAP treatment for both needle-type devices ( Figure 1 e,f). A range from 354 to 425 nm indicates the molecular transitions of the N2+ second positive system [ 47 ], whereas the characteristic emission peak of atomic oxygen appears at 777 nm [ 48 ]. According to this, the emission peaks observed in the in vivo and in vitro devices ( Figure 1 e,f) represent an atomic oxygen radical and an ionized nitrogen molecule, respectively; thereby, all of the results demonstrating the discharge parameters of the in vivo device are comparable to those of the in vitro device, and both of the devices can produce the same kinds of reactive oxides and nitrogen species (ROS/RNS).…”

Bactericidal effect of low-temperature atmospheric plasma against the Shigella flexneri

“…In the OES spectra of the discharge plasma, the strong emission of the second positive band N 2 , mainly composed of nitrogen molecules, was displayed at 340–410 nm. [ 25 ]. The second positive band N 2 is a well-known series of bands in nitrogen whose leptonic spectrum corresponds to the transition from the C 3 П μ electronic state to the B 3 П g electronic state.…”

Cold atmospheric plasma stabilizes mismatch repair for effective, uniform treatment of diverse colorectal cancer cell types

“…Optical emission spectroscopy (OES) primarily serves for the qualitative assessment of CAP's reactive oxides and nitrogen species, with substance identification relying on spectral peak positions ( Fig.s 8 ). Molecular transitions of the nitrogen second positive system (N 2 SPS) are indicated within the range of 354–425 nm (354.40 nm, 358.32 nm, 371.65 nm, 376.23 nm and 381.04 nm) 58 – 61 .The nitrogen first negative system (N 2 FNS) emissions are observed at wavelengths ranged 390–440 nm (392.01 nm, 394.75 nm, 400.45 nm, 406.59 nm, 420.43 nm, 427.45 nm and 434.67 nm) 58 – 60 . The second-order nitrogen molecules N 2 are observed at wavelengths ranged 595–775 nm (595.99 nm, 627.75 nm, 632.41 nm, 674.8 nm, 707.88 nm, 715.46 nm, 751.42 and 761.47 nm) 62 , 63 .The emissions, arising from excited nitrogen species, could result from nitrogen molecules present in both the feeding gas and the ambient environment.…”