Treatment of Diabetic Foot Ulcer (DFU) with Pharmaceutical Product using Hirudo orientalis: A Case Report

Amani, Leili; Fadaei, Fatemeh; Ardakani, Mohammadreza Shams; Ardakani, Mehran Mirabzadeh; Shirbeigi, Laila

doi:10.30699/jambs.28.129.225

Cited by 2 publications

(1 citation statement)

References 11 publications

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“…Diabetic foot ulcer is considered as one of the most important wound in the body having a complex treatment (22,23). There are several treatments for diabetic foot ulcers, among which the use of leeches is considered as one of the recommended methods (24). In general, reducing pain and inflammation is regarded as the first step in wound healing (25), where we can see re-epitelialization and angiogenesis (26).…”

Section: Discussionmentioning

confidence: 99%

Semi-Solid Product of Medicinal Leech Enhances Wound Healing in Rats

Amani¹,

Motamed²,

Ardakani³

et al. 2021

Jundishapur J Nat Pharm Prod

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Background: Wound causes a series of cascading reactions such as cellular damage, inflammatory reactions, and infection. Objectives: The present study aimed to prepare pharmaceutical skin product from lyophilized Hirudo orientalis leech source with active enzyme and proteins, having wound healing and anti-coagulation activities. Methods: The present study focused on the bioactive protein of Hirudo orientalis with anti-trypsin and anti-coagulation activity. First, biological active substance was prepared from lyophilized leech conducted on resultant 5% leech cream. Skin absorption of 5% leech cream was evaluated by Franz cell test, and in vivo wound healing capability of the leech cream was compared with phenytoin cream. In addition, the microbial control of final product was summed-up. Further, the quality control analysis and protein purification in final product were conducted using elution procedure on Hirudin protein-related region. Finally, the anticoagulant activity of the resultant protein was observed. Results: Using the result of the experiments investigating the successful effect containment function of the trypsin, we established the decreased rate of UV absorption in the area of 410 nm in question based on increases in the amount of cream. With 0, 100, 200 and 300 mg of 5% leech cream, absorbance (A) rate was: A (1.9, 1.47, 0.753 and 0.00) (P < 0.05; √2 = 21.6). In anticoagulation activity increased rate of partial thromboplastin time (PTT) (sec) (mean ± SEM), (26 ± 0.00, 34.22 ± 0.61 and 42 ± 0.71) with the 0, 100, and 200 mg final product was established. No bacterial colony growth was observed in the microbial culture analysis. In vivo results demonstrated that leech cream and phenytoin could promote wound regeneration by accelerating the re-epithelialization process and initial angiogenesis among all treatment groups. Conclusions: In the wound healing, we usually seek to reduce pain and inflammation, beside epitelialization and angiogenesis. Leech saliva contains biological substances that we have proven in this study. Our expectations for wound healing will be met with this pharmaceutical product citing the result of this study. The leech product proved to be the best selection for cosmetic with the added capabilities of renovation of the hair glands and regular skin form and typical depth of outer layer of skin. Thus, the leech cream can be considered as a better substitute for the conventional wound healing products.

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Section: Discussionmentioning

confidence: 99%

Semi-Solid Product of Medicinal Leech Enhances Wound Healing in Rats

Amani¹,

Motamed²,

Ardakani³

et al. 2021

Jundishapur J Nat Pharm Prod

Self Cite

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An overview of leech saliva and cosmetic potential

Çoruk,

Kaymak,

Ayhan

et al. 2024

Eurasian Journal of Biological and Chemical Sciences

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The therapeutic benefits of leech secretion have been known for centuries, and the potential of this substance in modern cosmetics is now attracting increasing interest. Leech secretion contains various bioactive compounds, including enzymes, peptides, and proteins. The bioactive components found in leech saliva, including anticoagulants, growth factors, and antimicrobial peptides, are responsible for leech saliva's effectiveness in increasing skin hydration, reducing inflammation, and promoting wound healing. To utilize their potential, it is imperative to sift through the skin area. This study reviews the available literature and research studies on the chemical properties of leech saliva and its use in cosmetic formulations. It highlights the bioactive compounds, their potential mechanisms of action, and their efficacy in cosmetic applications. The sources consulted include peer-reviewed articles, clinical studies, and industry reports. Data from these studies are synthesized to provide a comprehensive overview of the current state of knowledge. The findings suggest that leech saliva has significant potential as an ingredient in cosmeceutical products and offers new benefits for skin rejuvenation and repair. The review highlights the need for further research to optimize extraction processes, ensure product safety, and investigate additional applications. By advancing our understanding of leech saliva's chemical properties and mechanisms, future studies may facilitate the development of innovative skincare solutions and increase the efficacy of cosmetic formulations.

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Treatment of Diabetic Foot Ulcer (DFU) with Pharmaceutical Product using Hirudo orientalis: A Case Report

Cited by 2 publications

References 11 publications

Semi-Solid Product of Medicinal Leech Enhances Wound Healing in Rats

Semi-Solid Product of Medicinal Leech Enhances Wound Healing in Rats

An overview of leech saliva and cosmetic potential

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