Treatment of “En Coup de Sabre” Deformity With Adipose-Derived Regenerative Cell–Enriched Fat Graft

Karaaltın, Mehmet Veli; Akpinar, Ali Cem; Bağhaki, Semih; Akpinar, Fatma

doi:10.1097/scs.0b013e3182418ce8

Cited by 41 publications

(28 citation statements)

References 12 publications

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“…This is the first report in which this kind of therapeutic approach has been used to treat regional sclerotic changes in SSc. Some cases of linear scleroderma and morphea have been reported in which AFTG has been successfully used (10,17,18,33). Other cases of localized scleroderma, as well as other patients with different atrophic or sclerotic skin abnormalities, have been alternatively treated by transplantation of ATDMSCs (35).…”

Section: Discussionmentioning

confidence: 99%

“…More recently, some patients affected by linear scleroderma and different types of morphea have been treated by means of AFTG or by transplantation of adipose-derived mesenchymal stem cells (ATDMSCs) (10,17,18,33,35). In most of these cases, a significant increase of skin elasticity and thickening has been reported, with both aesthetic and functional improvement of the treated skin areas, while no serious side effects have been observed during and after these kinds of procedures.…”

Section: Introductionmentioning

confidence: 99%

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Autologous Fat Grafting in the Treatment of Fibrotic Perioral Changes in Patients with Systemic Sclerosis

et al. 2015

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Autologous fat tissue grafting (AFTG) has been successfully used in the treatment of different sclerotic conditions, including localized scleroderma. Patients with advanced systemic sclerosis (SSc)-related perioral thickening and mouth opening limitation are candidates for this therapeutic approach. AFTG of the lips was performed to improve mouth opening in patients with SSc. We enrolled in the study 20 female patients with diffuse SSc (median age 35 ± 15 years and 11 ± 10 years of disease duration). Two-milliliter fractions of autologous fat drawn from trochanteric or periumbilical areas were injected in eight different sites around the mouth. Baseline and after-treatment mouth opening changes were assessed by measuring interincisal distance and oral perimeter, while skin hardness was tested by digital durometer. Pre-and posttreatment modifications of microvascular architecture were assessed by counting capillaries in the inferior lip videocapillaroscopy (VC) images and by scoring the microvascular density (MVD) in anti-CD34/CD31 immunohistochemical (IH) stained perioral skin biopsy sections. Similarly, histological sections were examined to evaluate dermoepidermic junction (DEJ) modifications. Three months after treatment, both the interincisal distance and oral perimeter significantly increased (p < 0.001). At the same time, a significant skin neovascularization became evident, both considering the VC images (p < 0.001) and MVD scores in IH sections (p < 0.0001). Finally, some skin histological aspects also improved, as shown by the significant changes in DEJ flattening scores (p < 0.0001). The present study suggests that, in patients with SSc, AFTG can improve mouth opening and function, induce a neovascularization, and partially restore the skin structure.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Autologous Fat Grafting in the Treatment of Fibrotic Perioral Changes in Patients with Systemic Sclerosis

et al. 2015

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“…In recent years, fat grafts have been widely used in the treatment of the aging of facial skin to fill in surface depressions [5, 12-14]. Many clinicians have noted that implanted fat tissue produces rejuvenation effects on skin surrounding the transplantation area [1, 12, 13].…”

Section: Discussionmentioning

confidence: 99%

Precise Intradermal Injection of Nanofat-Derived Stromal Cells Combined with Platelet-Rich Fibrin Improves the Efficacy of Facial Skin Rejuvenation

Liang

Lü

et al. 2018

Cell Physiol Biochem

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Background/Aims: The rejuvenation properties of nanofat grafting have been described in recent years. However, it is not clear whether the clinical efficacy of the procedure is attributable to stem cells or linked to other components of adipose tissue. In this study we isolated nanofat-derived stem cells (NFSCs) to observe their biological characteristics and evaluate the efficacy of precise intradermal injection of nanofat combined with platelet-rich fibrin (PRF) in patients undergoing facial rejuvenation treatment. Methods: Third-passage NFSCs were isolated and cultured using a mechanical emulsification method and their surface CD markers were analyzed by flow cytometry. The adipogenic and osteogenic nature and chondrogenic differentiation capacity of NFSCs were determined using Oil Red O staining, alizarin red staining, and Alcian blue staining, respectively. Paracrine function of NFSCs was evaluated by enzyme-linked immunosorbent assay (ELISA) at 1, 3, 7, 14, and 28 days after establishing the culture. Then, the effects of PRF on NFSC proliferation were assessed in vitro. Finally, we compared the outcome in 103 patients with facial skin aging who underwent both nanofat and intradermal PRF injection (treatment group) and 128 patients who underwent hyaluronic acid (HA) injection treatment (control group). Outcomes in the two groups were compared by assessing pictures taken at the same angle before and after treatment, postoperative recovery, incidence of local absorption and cysts, and skin quality before treatment, and at 1, 12, 24 months after treatment using the VISIA Skin Image Analyzer and a SOFT5.5 skin test instrument. Results: NFSCs expressed CD29, CD44, CD49d, CD73, CD90, and CD105, but did not express CD34, CD45, and CD106. NFSCs also differentiated into adipocytes, osteoblasts, and chondrocytes under appropriate induction conditions. NFSCs released large amounts of growth factors such as VEGF, bFGF, EGF, and others, and growth factor levels increased in a time-dependent manner. At the same time, PRF enhanced proliferation of NFSCs in vitro in a dose-dependent manner, and the growth curves under different concentrations of PRF all showed plateaus 6d after seeding. Facial skin texture was improved to a greater extent after combined injection of nanofat and PRF than after control injection of HA. The nanofat-PRF group had a higher satisfaction rate. Neither treatment caused any complications such as infection, anaphylaxis, or paresthesia during long-term follow-up. Conclusion: NFSCs demonstrate excellent multipotential differentiation and paracrine function, and PRF promotes proliferation of NFSCs during the early stage after seeding. Both nanofat-PRF and HA injection improve facial skin status without serious complications, but the former was associated with greater patient satisfaction, implying that nanofat-PRF injection is a safe, highly effective, and long-lasting method for skin rejuvenation.

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“…13 In the literature there are at least three experimental studies demonstrating that supplementation of progenitor cells enhances the volume or weight of surviving adipose tissue, 6,[13][14][15][16] and first reports of its clinical use suggest that this approach may be feasible and effective for softtissue augmentation on the breast, face, hip, and hand. 12,[17][18][19][20][21][22] However, considering that these studies represent level IV evidence (which corresponds to the publication of case series), 23 there is a lack of randomized, controlled clinical trials comparing this method to current standard techniques. 24 Thus, this study aimed to fill this gap by investigating whether a faster protocol for isolation of adiposederived stromal cells and their use in combination with fat tissue improve the long-term retention of the grafts in patients with craniofacial microsomia.…”

Section: Fat Grafts Supplemented With Adipose-derived Stromal Cells Imentioning

confidence: 99%

Fat Grafts Supplemented with Adipose-Derived Stromal Cells in the Rehabilitation of Patients with Craniofacial Microsomia

Tanikawa

Aguena

Bueno

et al. 2013

Plastic and Reconstructive Surgery

127

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Treatment of “En Coup de Sabre” Deformity With Adipose-Derived Regenerative Cell–Enriched Fat Graft

Cited by 41 publications

References 12 publications

Autologous Fat Grafting in the Treatment of Fibrotic Perioral Changes in Patients with Systemic Sclerosis

Autologous Fat Grafting in the Treatment of Fibrotic Perioral Changes in Patients with Systemic Sclerosis

Precise Intradermal Injection of Nanofat-Derived Stromal Cells Combined with Platelet-Rich Fibrin Improves the Efficacy of Facial Skin Rejuvenation

Fat Grafts Supplemented with Adipose-Derived Stromal Cells in the Rehabilitation of Patients with Craniofacial Microsomia

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