Treatment of Hemophilia with Glycine-Precipitated Factor VIII

In vitro and in vivo Characterization of Factor VIII Preparations

“…Recovery may vary according to the factor VIII product, the patient and the estimation of factor VIII dose. This study confirms the findings which showed similar recovery with factor VIII concentrates of various purity [26,39], Pre vious studies have shown large variations in recovery from patient to patient [1,2,6,10,34]. The recovery of F VIII:C was not sig nificantly related to the clinical state of the bleeding or nonbleeding patient [1].…”

“…Both F VIIEAG and F VIIEVWF were in higher concentra tions than F VIIEC, the ratio of F VIIEAG/ F VIIEC being 2 to 3. These figures are somewhat lower than those indicated by oth- [1,15,26], who found for Hemophil method IV a F VIIEAG/F VIIEC ratio of 4.5 to 10. Another study found results simi lar to ours in several nonspecified products [40], This high F VIIEAG/F VIIEC ratio probably indicates a preferential denaturization of the most labile component of the fac tor VIII complex during purification.…”

“…In vivo recovery is determined by the ratio between either the F VIIFC plasma level measured at a given interval after infusion, or the highest level obtained in one of several early samples and the ex pected F VIIFC recovery. According to in vestigators, the time of sampling and there fore the assumed peak of F VIIFC plasma levels varies from 5 to 10 min to as late as 1-2 h postinfusion with cryoprecipitate and Cohn fraction I [2,3,31], with interme diate purity [1,16,26,31] and with high purity preparations [9,14,16,26], In this study, the F VIIFC plasma level was as sayed early (15 min) and late (30, 60 min) after infusion and in all cases the peak of F VIII:C was found at 1 h postinfusion. Al though all subjects had a hemorrhage at the time of infusion, it is unlikely that the clini cal status influenced the results.…”

6 Factor VIII Concentrates, Factor VIII/von Willebrand Factor Concentrates, Factor IX Concentrates, Activated Prothrombin Complex Concentrates

“…Additional isolation steps include either immunoaffinity chromatography, ion exchange chromatography or precipitation procedures [1,10,26,44]. Precipitation as well as chromatography lead to enrichment with functional vWf [12,43].…”

Evaluation of Dental Procedures on Hemophilic Patients in a Comprehensive Program for the Care of Hemophilia (Part 3)

“…The biological half-life of infused AHF is eight to 11 hours ; and after 22 hours it is only 20 per cent of the original AHF level. One must consider Abildgaard's [1] statement that an attempt to maintain AHF level may lead to congestive heart failure due to the overexpansion ofthe total blood volume. Kerr [46] has also stated that other complications which may arise are thrombophlebitis, generalized allergic reactions, proteinuria , and the formation of circulating inhibitors.…”