Treatment of melanoma lesions using methylene blue and RL50 light source

Tardivo, João Paulo; Giglio, Auro del; Paschoal, L. H. C.; Ito, Ayako; Baptista, Maurı́cio S.

doi:10.1016/s1572-1000(05)00005-0

Cited by 47 publications

(38 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The in-house light source RL50® [4] was employed. This emits light in the red region of the spectrum from 600-750 nm and overlaps well with the MB/TB action spectra.…”

Section: Light Irradiationmentioning

confidence: 99%

Small scale trial of photodynamic treatment of onychomycosis in São Paulo

Tardivo

Wainwright

Baptista

2015

Journal of Photochemistry and Photobiology B: Biology

View full text Add to dashboard Cite

show abstract

“…The in-house light source RL50® [4] was employed. This emits light in the red region of the spectrum from 600-750 nm and overlaps well with the MB/TB action spectra.…”

Section: Light Irradiationmentioning

confidence: 99%

Small scale trial of photodynamic treatment of onychomycosis in São Paulo

Tardivo

Wainwright

Baptista

2015

Journal of Photochemistry and Photobiology B: Biology

View full text Add to dashboard Cite

show abstract

“…O AM é um FS com características interessantes para uso em PDT (8)(9)(10). Possui alto rendimento quântico de cruzamento entre sistemas, podendo gerar concentrações elevadas de oxigênio singlete ( 1 O 2 ), principal espécie responsável pela indução de morte celular em PDT (8)(9)(10)(11)(12).…”

Section: Fotossensibilizadores E Fotossensibilizadores Fenotiazínicosunclassified

“…Possui alto rendimento quântico de cruzamento entre sistemas, podendo gerar concentrações elevadas de oxigênio singlete ( 1 O 2 ), principal espécie responsável pela indução de morte celular em PDT (8)(9)(10)(11)(12). AM pode também induzir a formação de radicais, que podem levar a danos em biomoléculas (8).…”

Section: Fotossensibilizadores E Fotossensibilizadores Fenotiazínicosunclassified

“…AM pode também induzir a formação de radicais, que podem levar a danos em biomoléculas (8). Possui citotoxicidade no escuro relativamente baixa além de ser economicamente acessível (10). Resultados positivos da aplicação clínica de AM tem sido obtidos (10,11).…”

Section: Fotossensibilizadores E Fotossensibilizadores Fenotiazínicosunclassified

“…Possui citotoxicidade no escuro relativamente baixa além de ser economicamente acessível (10). Resultados positivos da aplicação clínica de AM tem sido obtidos (10,11). Na figura 2, observa-se o efeito da PDT com AM em melanoma, onde se percebe o desaparecimento total de um dos tumores e a redução significativa do outro.…”

Section: Fotossensibilizadores E Fotossensibilizadores Fenotiazínicosunclassified

See 2 more Smart Citations

Eficiência fotodinâmica dos fenotiazínicos em mitocôndrias e células tumorais

Gabrielli¹

View full text Add to dashboard Cite

Developing an analytical method by HPLC for simultaneous quantification of methylene blue and metformin applied to in vitro skin permeation and retention studies

Matos

Calixto

Garcı́a

2021

Biomedical Chromatography

View full text Add to dashboard Cite

The aim of this study was to develop an HPLC method for simultaneous quantification of metformin (MET) and methylene blue (MB) in in vitro skin permeation/retention studies, in which retention was evaluated in the different layers of the skin [stratum corneum (SC) and the viable epidermis + dermis (VE + D)]. The method was validated considering the following parameters: specificity, linearity, quantitation limit (LOQ), recovery, precision and accuracy. Calibration curves were obtained using the following six matrices: methanol, water, methanolic extracts from the SC and VE + D spiked with the drugs and drugs extracted from the SC and VE + D. The precision, accuracy and LOQ of the method were evaluated in water and in VE + D and SC, applying the drug extraction process. The results show that the method is selective and linear for both drugs. The precision and accuracy values, independent of matrix and drug, were below the limit of 15%. The LOQ of MB was defined as 0.4 μg/ml in the VE + D and SC and 0.8 μg/ml in water. The LOQ of MET was defined as 0.8 μg/ml in the VE + D and SC and 0.4 μg/ml in the water. The recovery of the method was adequate, consistent and reproducible for the concentration range of 0.4-10 μg/ml for MB (73.3-92.1%) and 0.8-10.0 μg/mL for . This method has a potential application in the development of formulation for skin delivery of MB and MET.

show abstract

Treatment of melanoma lesions using methylene blue and RL50 light source

Cited by 47 publications

References 7 publications

Small scale trial of photodynamic treatment of onychomycosis in São Paulo

Small scale trial of photodynamic treatment of onychomycosis in São Paulo

Eficiência fotodinâmica dos fenotiazínicos em mitocôndrias e células tumorais

Developing an analytical method by HPLC for simultaneous quantification of methylene blue and metformin applied to in vitro skin permeation and retention studies

Contact Info

Product

Resources

About