Treatment of Mice with Naringin Alleviates the Doxorubicin-Induced Oxidative Stress in the Liver of Swiss Albino Mice

Abstract

Doxorubicin a photosensitive antibiotic is frequently used to treat various malignancies in clinics; however, doxorubicin is also taken up by the normal cells of the body leading to increased oxidative stress and subsequently DNA damage and dysfunction in the cells. The agents which can reduce the adverse effect of doxorubicin will of great value. The present study has attempted to study the effect of naringin a citrus flavonoid on the doxorubicininduced oxidative stress in mice liver. The mice were administered with 0, 1, 5 or 10mg/ kg body weight of doxorubicin and treated with 10mg/kg body weight of naringin before or after doxorubicin treatment. The glutathione concentration and lipid peroxidation and activities of glutathione-s-transferase, catalase and super oxide dismutase were studied at 0.5, 1, 2 and 4h post-treatment in the mouse liver. The doxorubicin increased the lipid peroxidation in a dose dependent manner at all the post treatment times. Conversely, it attenuated the activities of glutathione-s-transferase, catalase and super oxide dismutase in dose dependent fashion and time dependent manner. A similar effect was observed for glutathione concentration. Treatment of mice with naringin before or after doxorubicin treatment elevated all the antioxidants and reduced the doxorubicin -induced lipid peroxidation. The effect of naringin pretreatment was more effective when compared to its post treatment. The naringin was very effective in reducing the oxidative stress induced by doxorubicin as indicated by the elevated levels of glutathione concentration, glutathiones-transferase, catalase and super oxide dismutase. The pretreatment of naringin was better than its post treatment.

Naringin protects rat lung against the doxorubicin-induced biochemical injury

“…32,56 The earlier studies on mice has also reported a decline in the GSH contents in the heart, liver and bone marrow cells. 24,33,34,57 Naringin treatment arrested the doxorubicin decline in the rat lung in the present study. Earlier naringin has been reported to alleviate the doxorubicin-induced decline in the GSH concentration in rat liver and mouse liver and bone marrow cells.…”

“…Earlier naringin has been reported to alleviate the doxorubicin-induced decline in the GSH concentration in rat liver and mouse liver and bone marrow cells. 24,33,34,58,59 The extract of Aegle mermelos has also been reported to increase the GSH contents in mice heart in an earlier study. 57 An amino acid glutamine has been also reported to protect rats against Doxorubicin-induced attrition in GSH contents.…”

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“…The single injection (i.p) of DOX to mice results in significant depletion of antioxidants activity, which is in agreement with earlier reports from this laboratory in rat and mice liver 48,49 . Alleviation in GSH level might be due to its increased utilization by the hepatocytes in scavenging doxorubicin metabolites.…”

“…In the present study, a significant increase (61.97%) in MDA levels in liver cells was observed in DOX-treated animals. Similarly, DOX has been reported to increase lipid peroxidation in rat and mouse liver 48,49 . The increased LOO could be attributed to the overproduction of superoxide anion and reduction in detoxifying hyperperoxides.…”

“…Naringin a citrus bioflavonoid has been reported to increase GSH and activities of GST, SOD and CAT in the rat and mice liver earlier 48,49 . Since lipid peroxidation (LOO) is a free radical oxidation product of polysaturated fatty acids, detection and measurement of LOO is the evidence which is frequently cited to support the involvement of freeradicals reactions in toxicity and disease progression 51 .…”