Treatment of Microcotyle sebastis infestation in cultured rockfish Sebastes schlegeli by oral administration of praziquantel in combination with cimetidine

Abstract

The effect of cimetidine on the treatment efficacy of praziquantel against Microcotyle sebastis infestation in cultured rockfish Sebastes schlegeli was investigated. Juvenile rockfish were divided into 7 groups, and orally administered praziquantel alone (50, 100 and 200 mg kg -1 body wt, BW) or in combination with cimetidine at a dose of 200 mg kg -1 BW for each praziquantel dose. The fish in the control group were administered only saline. The results clearly showed that coadministration of cimetidine with praziquantel led to a significantly increased treatment efficacy of the latter drug, and consequently would lead to a lowering of the total dose of praziquantel, and a reduction in the administration times and costs for the treatment of M. sebastis infestation in cultured rockfish. KEY WORDS: Praziquantel · Cimetidine · Microcotyle sebastis · Sebastes schlegeli · Treatment efficacy Resale or republication not permitted without written consent of the publisherDis Aquat Org 44: [133][134][135][136] 2001 scribed an increase in praziquantel levels in plasma of mammals when cimetidine is simultaneously administered (Dachman et al. 1994, Ebeid et al. 1994, Metwally et al. 1995, Jung et al. 1997.In the present study, we investigated the treatment efficacy of praziquantel against Microcotyle sebastis infestation in cultured rockfish when administered simultaneously with cimetidine. MATERIALS AND METHODSFish. Netpen-reared juvenile rockfish Sebastes schlegeli (body length: 13 to 16 cm) were obtained from a local rockfish farm in Tongyoung, Korea. The presence of Microcotyle sebastis on the gills was confirmed by examination of 10 fish. After 1 wk acclimation, a total of 70 fish was randomly divided into 7 groups of 10 fish in each group. The volume of each experimental aquarium was 50 l, the water temperature was 20 ± 1°C, and salinity was 33 ‰. Fish were not fed throughout the experiment.Treatment regime. Fish were anaesthetized with MS222 (Sigma) and were intubated directly onto the stomach with varying concentrations of praziquantel and cimetidine. The first group was given 200 mg praziquantel (Shinpoong Pharm. Co. Ltd.) kg -1 BW, the second group 100 mg praziquantel kg -1 BW, and the third group 50 mg praziquantel kg -1 BW. The fourth group was coadministered 200 mg praziquantel and 200 mg cimetidine kg -1 BW, the fifth group 100 mg praziquantel and 200 mg cimetidine kg -1 BW, and the sixth group 50 mg praziquantel and 200 mg cimetidine kg -1 BW. The fish in the seventh group (control group) were given 0.7% saline. At 84 h post-treatment, the gills of all fish in each group were examined to confirm the effectiveness of each treatment. Abundance and prevalence were determined according to the methods given in Margolis et al. (1982).Statistical analysis. All data were analyzed using Mann-Whitney's U-test (SPSS for Windows, SPSS Inc.). RESULTSPrevalence and intensity of Microcotyle sebastis in the fish sampled randomly before the start of the treatments were 100% and 21.9 ± 16.8 (mean ± SD), respective...

Cimetidine enhances the plasma praziquantel concentration and treatment efficacy against Microcotyle sebastis in cultured rockfish Sebastes schlegeli

“…The results of the present study agree with a previous report which indicated that coadministration of cimetidine with praziquantel led to a significantly increased treatment efficacy of the latter drug against Microcotyle sebastis infestation in rockfish Sebastes schlegeli (Kim et al 2001a). Moreover, in the present study we first demonstrated in fish that these increased treatment efficacies by cimetidine supplementation were through elevation of praziquantel concentration in the blood of treated fish.…”

“…Although Kim et al (2001a) have reported that coadministration of cimetidine with praziquantel led to a significantly increased treatment efficacy of the latter drug against Microcotyle sebastis, there are no experimental data on the pharmacokinetic interactions between praziquantel and cimetidine in fish. Therefore, ABSTRACT: The effect of cimetidine on the praziquantel concentration in the blood of the rockfish Sebastes schlegeli and the consequent effect on the treatment efficacy against Microcotyle sebastis were investigated.…”

The effect of praziquantel applied per os on selected haematological and biochemical indices in common carp (Cyprinus carpio L.)

“…Praziquantel is a parasiticide that is effective against tapeworms, monogeneans, and trematodes (Buchmann 1987;Mitchell 1995;Kim et al 2001;Sharp et al 2004). It was the first anthelminthic to fulfil the WHO requirements for population-based chemotherapy to treat a broad range of parasitic infections (Wegner 1981).…”

Efficacy of orally administered praziquantel against Zeuxapta seriolae and Benedenia seriolae (Monogenea) in yellowtail kingfish Seriola lalandi

“…Recent studies have investigated its effect on the polyopisthocotyleans Microcotyle sebastis (Kim et al 1998, 2001, Kim & Cho 2000, Kim & Kim 2002, Heterobothrium okamatoi (Hirazawa et al 2000) and Sparicotyle chysophrii (Sitjà-Bobadilla et al 2006), as well as the monopisthocotyleans Gyrodactylus sp. (Tojo & Santamarina 1998) and Neobenedenia girellae (Hirazawa et al 2004).…”