Treatment of Pterygium by Ligation and Bevacizumab Injection

Nakasato, Houmei; Uemoto, Riyo; Mizuki, Nobuhisa

doi:10.1097/ico.0b013e3182408bc7

Cited by 6 publications

(4 citation statements)

References 9 publications

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“…However, no study to date has measured changes in capillary hemodynamics alongside changes in protein expression at the level of a single vessel segment or over time. To fill this void, we modified an established vessel ligation technique 37 , 41 to alter blood flow in limbal vessels, and we applied this technique to the corneal neovasculature to demonstrate the ability to measure changes in capillary hemodynamics and endothelial cell protein expression in individual neovessel segments across sequential time points (Fig. 2a ).…”

Section: Resultsmentioning

confidence: 99%

Dynamic, heterogeneous endothelial Tie2 expression and capillary blood flow during microvascular remodeling

Kelly-Goss

Ning

Bruce

et al. 2017

Sci Rep

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Microvascular endothelial cell heterogeneity and its relationship to hemodynamics remains poorly understood due to a lack of sufficient methods to examine these parameters in vivo at high resolution throughout an angiogenic network. The availability of surrogate markers for functional vascular proteins, such as green fluorescent protein, enables expression in individual cells to be followed over time using confocal microscopy, while photoacoustic microscopy enables dynamic measurement of blood flow across the network with capillary-level resolution. We combined these two non-invasive imaging modalities in order to spatially and temporally analyze biochemical and biomechanical drivers of angiogenesis in murine corneal neovessels. By stimulating corneal angiogenesis with an alkali burn in Tie2-GFP fluorescent-reporter mice, we evaluated how onset of blood flow and surgically-altered blood flow affects Tie2-GFP expression. Our study establishes a novel platform for analyzing heterogeneous blood flow and fluorescent reporter protein expression across a dynamic microvascular network in an adult mammal.

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Section: Resultsmentioning

confidence: 99%

Dynamic, heterogeneous endothelial Tie2 expression and capillary blood flow during microvascular remodeling

Kelly-Goss

Ning

Bruce

et al. 2017

Sci Rep

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“…Nakasato et al. () reported a series of four cases in which they injected the bevacizumab into the pterygial base, followed by ligation of the pterygium neck with an 8–0 Ethicon suture. This method resulted in regression of the pterygial head (complete) and body (partial) 1 month after treatment, with zero recurrences during follow‐up.…”

Section: Resultsmentioning

confidence: 99%

Use of anti‐vascular endothelial growth factor in the management of pterygium

Mak

Chan

Marcet

et al. 2016

Acta Ophthalmologica

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ABSTRACT.The rising success of anti-vascular endothelial growth factor (VEGF) therapies in ocular disease has stimulated the use of such treatments in the surgical management of pterygium. We reviewed the literature to better understand the safety and efficacy of the adjunctive role of anti-VEGF treatments for pterygium excision. Without surgery, anti-VEGF alone may favourably alter symptoms and vascularity, but does not cause pterygium regression. Some evidence supports the use of anti-VEGF as an adjuvant therapy to surgery, especially when using a higher dose and a more frequent dosing regimen. Overall, anti-VEGF is generally safe and well tolerated in patients with pterygium. Currently, the evidence does not conclusively support the use of anti-VEGF in pterygium surgery. However, further research may guide unanswered questions regarding the interaction between VEGF and other factors responsible for pterygium growth. In addition, the optimal route and dosage of anti-VEGF administration is not yet known.

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“…Oxidative stress is considered a major pathogenesis of pterygium, there are other causes, for example, ultraviolet radiation-induced DNA injury, limbal stem cells deficiency (LSCD) [1] – [5] while the mechanism of pterygium is not fully understood. Meanwhile, there is no effective medication to treat pterygium or prevent the development of pterygium, the current main treatment is to remove the pterygium by surgery and the relapse rate after surgery is high [6] , [7] .…”

Section: Introductionmentioning

confidence: 99%

SERPINA3K Plays Antioxidant Roles in Cultured Pterygial Epithelial Cells through Regulating ROS System

Zhu

Pan

et al. 2014

PLoS ONE

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We recently demonstrated that SERPINA3K, a serine proteinase inhibitor, has antioxidant activity in the cornea. Here we investigated the antioxidant effects of SERPINA3K on the pterygial, which is partially caused by oxidative stress in pathogenesis. The head part of primary pterygial tissue was dissected and then cultured in keratinocyte serum-free defined medium (KSFM). The cultured pterygial epithelial cells (PECs) were treated with SERPINA3K. The cell proliferation and migration of PECs were measured and analyzed. Western blot and quantitative real-time polymerase chain reaction (PCR) assay were performed. It showed that SERPINA3K significantly suppressed the cell proliferation of PECs in a concentration-dependent manner, compared with cultured human conjunctival epithelial cells. SERPINA3K also inhibited the cell migration of PECs. Towards its underlying mechanism, SERPINA3K had antioxidant activities on the PECs by significantly inhibiting NADPH oxidase 4 (NOX4), which is an important enzyme of ROS generation, and by elevating the levels of key antioxidant factors of ROS: such as NAD(P)H dehydrogenase (quinone 1) (NQO1), NF-E2–related factor-2 (NRF2) and superoxide dismutases (SOD2). Meanwhile, SERPINA3K down-regulated the key effectors of Wnt signaling pathway: β-catenin, nonphospho-β-catenin, and low-density lipoprotein receptor-related protein 6 (LRP6). We provided novel evidence that SERPINA3K had inhibitory effects on pterygium and SERPINA3K played antioxidant role via regulating the ROS system and antioxidants.

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Treatment of Pterygium by Ligation and Bevacizumab Injection

Abstract: Although only 4 cases were studied, the uniform finding demonstrated that combined subpterygial bevacizumab injection and pterygial ligation is a potential effective procedure for removing pterygium.

Cited by 6 publications

References 9 publications

Dynamic, heterogeneous endothelial Tie2 expression and capillary blood flow during microvascular remodeling

Dynamic, heterogeneous endothelial Tie2 expression and capillary blood flow during microvascular remodeling

Use of anti‐vascular endothelial growth factor in the management of pterygium

SERPINA3K Plays Antioxidant Roles in Cultured Pterygial Epithelial Cells through Regulating ROS System

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