Treatment of rat pancreatic islets with reactive PEG

Panza, Janice L.; Wagner, William R.; Rilo, Horacio; Rao, R. Govinda; Beckman, Eric J.; Russell, Alan J.

doi:10.1016/s0142-9612(99)00283-5

Cited by 93 publications

(74 citation statements)

References 18 publications

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“…Polyethylene glycol (PEG) is used medically for injectable drugs 15,16) . Since PEGs absorb water and form a hydrogel capsule, the time required for drug release and metabolism is prolonged.…”

Section: Discussionmentioning

confidence: 99%

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Priming Effects of Triethylene Glycol and Triethylene Glycol Monomethacrylate on Dentin Bonding

Kusunoki¹,

Itoh²,

Utsumi³

et al. 2007

Dent. Mater. J.

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The objective of this study was to evaluate the effects of triethylene glycol (TEG) and triethylene glycol monomethacrylate (TEGMA) solutions as dentin primers on dentin bonding. To this end, wall-to-wall polymerization contraction gap width of a resin composite in a cylindrical dentin cavity and shear bond strength to a flat dentin surface were measured. Dentin was pretreated with an experimental dentin bonding system -consisting of 0.5 M ethylenediaminetetraacetic acid conditioner, TEG or TEGMA primer, and Clearfil Photo Bond bonding agent -prior to resin composite filling. When the cavity was primed with an aqueous solution of 35 vol％ TEG, 35 or 45 vol％ TEGMA for a few seconds, contraction gap formation was prevented completely. Then, among these three gap-free groups, there were no significant differences in shear bond strength. It was thus shown that both TEG and TEGMA were highly effective dentin primers, completely preventing contraction gap formation even when they were applied for only a few seconds.

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Section: Discussionmentioning

confidence: 99%

“…It is known that drug release is delayed when the drug is callused with polyethylene glycol (PEG) because of the water absorption properties of PEG 15,16) . Consequently, it is possible to reduce the frequency of injections by drug pegylation.…”

Section: Introductionmentioning

confidence: 99%

Priming Effects of Triethylene Glycol and Triethylene Glycol Monomethacrylate on Dentin Bonding

Kusunoki¹,

Itoh²,

Utsumi³

et al. 2007

Dent. Mater. J.

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“…PEGylation has been used for camouflaging islet surfaces to immunologically protect transplanted islets from the immune system (Panza et al, 2000;Lee et al, 2002;Lee et al, 2004). Methoxy-PEG-succinimidyl propionic acid molecules conjugated with amino groups of collagen matrix at the islet surface through a stable amide bond protected islets from immune cell attack (Fig.…”

Section: Covalent Conjugation Of Molecules To Islet Surfacesmentioning

confidence: 99%

“…Two major approaches have been introduced to prevent immunogenic reactions on the islet surfaces: macro and microencapsulation of the islet cells and islet cell surface modification ( Fig. 1) Panza et al, 2000;Scott & Murad, 1998;Opara et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Encapsulation and Surface Engineering of Pancreatic Islets: Advances and Challenges

Kozlovskaya¹,

Zavgorodnya²,

Kharlampieva³

2012

Biomedicine

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“…[6][7][8][9][10] This approach has more recently been extended to tissue and cell surfaces to inhibit acute thrombosis and attenuate immune recognition. [11][12][13][14][15][16] In the current study, N-hydroxysuccinimide-polyethylene glycol-biotin (NHS-PEG-biotin) was employed to covalently modify cellular and vascular surfaces. The active NHS ester of PEG reacts with primary amines, the most common being the epsilon amine of lysine, which can covalently link the PEG-biotin molecule to proteins found on cell membranes or extracellular matrix [ Fig.…”

Section: Introductionmentioning

confidence: 99%

Targeting microspheres and cells to polyethylene glycol‐modified biological surfaces

Deglau¹,

Johnson²,

Villanueva³

et al. 2006

J Biomedical Materials Res

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It has previously been demonstrated that damaged arterial tissue can be acutely modified with proteinreactive polyethylene glycol (PEG) to block undesirable platelet deposition. This concept might be expanded by employing PEG-biotin and its strong interaction with avidin for site-specific targeted delivery. Toward this end, cultured endothelial cells (ECs) were surface modified with PEG-biotin and the available biotin was quantified with flow cytometry. NeutrAvidin-coated microspheres and PEG-biotin modified ECs with NeutrAvidin as a bridging molecule were delivered under arterial shear stress to PEG-biotin modified ECs on a coverslip as well as scrape-damaged bovine carotid arteries. After incubation with a 10 mM solution for 1 min, 8 × 10 7 PEG-biotin molecules/EC were found and persisted for up to 120 h. Perfused microspheres adhered to NHS-PEG-biotin treated bovine carotid arteries with 60 ± 16 microspheres/mm 2 versus 11 ± 4 microspheres/mm 2 for control arteries (p < 0.015). Similarly, 22 ± 5 targeted ECs/mm 2 adhered to NHS-PEG-biotin treated bovine carotid arteries versus 6 ± 2 ECs/mm 2 for control arteries (p < 0.01). The targeting strategy demonstrated here might ultimately find application for drug delivery, gene therapy, or cell therapy where localization to specific labeled vascular regions is desired following catheter-based or surgical procedures.

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Treatment of rat pancreatic islets with reactive PEG

Cited by 93 publications

References 18 publications

Priming Effects of Triethylene Glycol and Triethylene Glycol Monomethacrylate on Dentin Bonding

Priming Effects of Triethylene Glycol and Triethylene Glycol Monomethacrylate on Dentin Bonding

Encapsulation and Surface Engineering of Pancreatic Islets: Advances and Challenges

Targeting microspheres and cells to polyethylene glycol‐modified biological surfaces

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