Treatment with an aromatase inhibitor induces complete sex change in the protogynous honeycomb grouper (Epinephelus merra)

Bhandari, Ramji Kumar; Higa, Motoki; Komuro, Hiroki; Nakamura, Shigeo; Nakamura, Masaru

doi:10.1023/b:fish.0000030501.23595.2e

Cited by 9 publications

(10 citation statements)

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“…The HPG axis has also been reported to be associated with sex change in protogynous fishes. Inhibition of estrogen biosynthesis in the gonad promotes female-to-male sex change (Devlin and Nagahama, 2002;Bhandari et al, 2003;Alam et al, 2006;Li et al, 2006;Nozu et al, 2009). Injection of mammalian LH or human chorionic gonadotropin (hCG), which interacts with LH receptor in fish, induces the appearance of testicular tissue, resulting in sex change in rice-field eel, bluehead wrasse, and C. julis (Tang et al, 1974;Koulish and Kramer, 1989;Reinboth and Brusle-Sicard, 1997).…”

Section: Discussionmentioning

confidence: 99%

Molecular Cloning and Expression Profile of Sex-Specific Genes, Figla and Dmrt1, in the Protogynous Hermaphroditic Fish, Halichoeres Poecilopterus

2012

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The genes folliculogenesis specific basic helix-loop-helix (facor in the germline alpha, Figla) and doublesex and mab-3 related transcription factor 1 (Dmrt1) are female- and male-specific genes that play key roles in sex differentiation. To obtain a better understanding of the molecular mechanisms underlying female-to-male sex change, we cloned the cDNAs of these genes from an ovary and a testis of the protogynus wrasse, Halichoeres poecilopterus. This fish has two isoforms of Dmrt1, Dmrt1a and Dmrt1b, caused by an alternative splicing. The Dmrt1b has an insertion of three nucleotides (CAG) in the open reading frame. Figla and Dmrt1 displayed gonadal-specific expression and abundant in the ovaries and in the testes, respectively. In particular, levels of Figla expression in the ovaries were higher in the spawning season than in the non-spawning season. Once sex change began, Figla mRNA decreased and Dmrt1 mRNA increased with progression of oocyte degeneration and spermatogenesis. These expression levels were maintained until the completion of the sex change. Low Figla and high Dmrt1 were also observed in testes of primary males, which functioned as a gonochoristic male throughout its life span in this wrasse. The results of this study suggest that these genes may regulate the gonadal transition from ovary to testis by the same mechanism as that of formation and maintenance of the primary testis in H. poecilopterus.

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Section: Discussionmentioning

confidence: 99%

Molecular Cloning and Expression Profile of Sex-Specific Genes, Figla and Dmrt1, in the Protogynous Hermaphroditic Fish, Halichoeres Poecilopterus

2012

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“…In the saddleback wrasse, Thalassoma duperrey , the plasma levels of estradiol 17β (E 2 ) rapidly decrease during the onset of sex change, and the in vitro production of E 2 in the gonads is lower in the sex‐changed fish than in females (Nakamura et al, ). Moreover, blocking estrogen synthesis by administrating an aromatase inhibitor (AI) leads to both behavioral and morphological sex change in the three‐spot wrasse H. trimaculatus (Higa et al, ) and the grouper Epinephelus merra (Bhandari et al, ). On the other hand, administration of estrogen to sexually mature wrasse IP‐males induces ovarian differentiation (Kojima et al, ).…”

Section: Introductionmentioning

confidence: 99%

Characterization of gonadal soma‐derived factor expression during sex change in the protogynous wrasse, Halichoeres trimaculatus

Horiguchi

Nozu

Hirai

et al. 2013

Developmental Dynamics

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Background: Sex change in fishes provides a good experimental model for understanding the mechanisms and plasticity of sex determination and differentiation. The three-spot wrasse, Halichoeres trimaculatus is a protogynous hermaphrodite. During sex change from female to male, the ovary is replaced by the testis through the degeneration of oocytes and subsequent spermatogenesis. In the present study, we cloned a cDNA-encoding gonadal soma-derived factor (GSDF) from protogynous wrasse and examined its expression pattern in the sexually mature gonads and the sex-changing gonad induced experimentally by aromatase inhibition. Results: Expression of gsdf was predominantly observed in the testis, and it was mainly localized to the supporting cells surrounding the spermatogonia. In the ovary, only slight expression of gsdf was observed in morphologically undifferentiated supporting cells in contact with oogonia. During sex change, strong expression of gsdf appeared first in the supporting cells surrounding the gonial germ cells before the onset of spermatogenesis. Thereafter, the expression of gsdf continually increased in the supporting cells surrounding the proliferating spermatogonia throughout the sex change. Conclusions: These results suggest that gsdf is involved in the proliferation of spermatogonia and subsequent spermatogenesis in both the testis and the gonad in the early stages of sex change. Developmental Dynamics 242:388-399, 2013. V C 2013 Wiley Periodicals, Inc.Key words: gsdf; sex change; protogyny; sexual plasticity; three-spot wrasse Key findings:Gsdf is predominantly expressed in the testis of protogynous wrasse and is mainly localized to the supporting cells surrounding the spermatogonia. Only slight expression of gsdf was observed in most of the supporting cells in the neighboring ovarian oogonia. Strong expression of gsdf appeared in the supporting cells surrounding the gonial germ cells, which are the originators of spermatogenic cells in the ovary, during sex change induced by aromatase inhibition. These results suggest that gsdf is involved in the proliferation of spermatogonia and subsequent spermatogenesis in the early stages of sex change.

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“…The factor(s) which initiate sex change from female to functional males remains unknown (Bhandari et al . ). Decreasing of hormone estradiol (E2) and degeneration of vitellogenic of oocyte in wrasse Thalassoma duperrey during the sex reversal has been observed (Nakamura et al ., ).…”

Section: Introductionmentioning

confidence: 97%

“…While many researchers are involved in the study of sex reversal on fish, the mechanism of sex change on protogenous hermaphrodites is still unclear (Bhandari et al . ).…”

Section: Introductionmentioning

confidence: 97%

“…One special characteristic of grouper is their hermaphrodite progeny whereby the fish mature initially as females and subsequently, selectively change their sex to male. The factor(s) which initiate sex change from female to functional males remains unknown (Bhandari et al 2003). Decreasing of hormone estradiol (E2) and degeneration of vitellogenic of oocyte in wrasse Thalassoma duperrey during the sex reversal has been observed (Nakamura et al, 1989).…”

Section: Introductionmentioning

confidence: 99%

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Cryopreservation of spermatozoa on grouper species: a review

Che-Zulkifli

Koh

Shahreza

et al. 2018

Reviews in Aquaculture

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Cryopreservation of spermatozoa of grouper species supporting artificial insemination in production of fry has contributed to the grouper aquaculture industry. Grouper is a high‐value species in marine cage finfish culture and a major business for the aquaculture industry in South East Asia. The species contributes a significant value to the income of South East Asian countries such as Indonesia, Malaysia, Philippines and Thailand. Hybrid grouper is a new candidate for marine finfish culture, and cryopreservation of grouper and giant grouper spermatozoa is important to support development of its production. This review article discusses the cryopreservation of the spermatozoa of grouper species specifically. Emphasis is placed on the protocols for implementation and implications of the cryopreservation of grouper spermatozoa in the marine finfish aquaculture industry.

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Treatment with an aromatase inhibitor induces complete sex change in the protogynous honeycomb grouper (Epinephelus merra)

Cited by 9 publications

References 1 publication

Molecular Cloning and Expression Profile of Sex-Specific Genes, Figla and Dmrt1, in the Protogynous Hermaphroditic Fish, Halichoeres Poecilopterus

Molecular Cloning and Expression Profile of Sex-Specific Genes, Figla and Dmrt1, in the Protogynous Hermaphroditic Fish, Halichoeres Poecilopterus

Characterization of gonadal soma‐derived factor expression during sex change in the protogynous wrasse, Halichoeres trimaculatus

Cryopreservation of spermatozoa on grouper species: a review

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