Treatment with captopril abrogates the altered expression of alpha1 macroglobulin and alpha1 antiproteinase in sera of spontaneously hypertensive rats

Abstract

BackgroundProteins that are associated with hypertension may be identified by comparing the 2-dimensional gel electrophoresis (2-DE) profiles of the sera of spontaneously hypertensive rats (SHR) with those generated from normotensive Spraque-Dawley rats (SDR).ResultsFive proteins of high abundance were found to be significantly altered when the 2-DE serum profiles of the SHR were compared to those that were similarly generated from the SDR. Analysis by mass spectrometry and database search identified the proteins as retinol binding protein 4, complement C3, albumin (19.9 kDa fragment), alpha1 macroglobulin and alpha1 antiproteinase, which are all known to be associated with hypertension. The altered expression of the two latter proteins was found to be abrogated when similar analysis was performed on sera of the SHR that were treated with captopril.ConclusionOur data suggests that serum alpha1 macroglobulin and alpha1 antiproteinase are potentially useful complementary biomolecular indicators for monitoring of hypertension.

Anti-angiotensin converting enzyme (ACE) proteins from mycelia of Ganoderma lucidum (Curtis) P. Karst

“…Protein bands from SDS-PAGE gel were excised and subjected to in-gel tryptic digestion (6 ng/μL, 37°C overnight). Digested samples were then desalted using Zip Tip (C18) (Millipore) and analysed using 4800 Plus MALDI TOF/TOF MS (Applied Biosystems) [ 10 ] combined with Mascot database software (available at http://www.matrixscience.com ). Unmatched masses from the Mascot database search were further analysed by an alternative protein identification tool, ProFound (available at http://prowl.rockefeller.edu/prowl-cgi/profound.exe ) using the following parameters: taxonomy category - fungi, protein mass range - 0 kDa to 100 kDa, protein pI range - 3 to 10, one missed cleavage site, charge state of MH+, mass tolerance between 0.5 Da to 1.15 Da.…”

Metalloproteomic and differential expression in plasma in a rat model of type 1 diabetes

“…Alpha-1-antiproteinase protects the connective tissue from inflammatory enzymes and helps to prevent blood clotting, which could be related to why this protein was more highly expressed in group C than in group DM1. Levels of serum acutephase protein groups were shown to correlate positively with blood pressure in humans [30]; this is one of the most abundant proteins in the urine of patients with proteinuria, and has been suggested as a marker of glomerulopathy. The somewhat increased expression of this protein in DM1 compared with DM1 + I may be due to some secondary complication of the disease.…”