Tree xylem water isotope analysis by Isotope Ratio Mass Spectrometry and laser spectrometry: A dataset to explore tree response to drought

Carrière, Simon Damien; Martin‐StPaul, Nicolas; Cakpo, Coffi Belmys; Patris, Nicolas; Gillon, Marina; Chalikakis, Konstantinos; Doussan, Claude; Olioso, Albert; Babic, Milanka; Jouineau, Arnaud; Simioni, Guillaume; Davi, Hendrik

doi:10.1016/j.dib.2020.105349

Cited by 9 publications

(11 citation statements)

References 12 publications

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“…Previous studies suggested that these isotopic offsets would result from an isotopic fractionation caused by root morphological adaptations to xeric or saline environments that would force the water flow through the symplastic (cell-to-cell transport through walls and membranes) rather than the apoplastic (extracellular) pathway (Ellsworth and Williams, 2007;Poca et al, 2019). However, in the past decade, many studies have reported similar isotopic offsets between plant and source water in various biomes, including plants typical of temperate and humid ecosystems (Barbeta et al, 2019;Brooks et al, 2010;Brum et al, 2019;Carrière et al, 2020;De Deurwaerder et al, 2018;Evaristo et al, 2016;Geris et al, 2015;Tetzlaff et al, 2021), in addition to controlled experiments (Barbeta et al, 2020;Vargas et al, 2017). Much of this lit-erature overlooks these plant source water isotopic offset (Anderegg et al, 2013;Muñoz-Villers et al, 2018), whereas other studies acknowledge these offsets and attribute them to either missing water sources not sampled in the field (Bowling et al, 2017) or to the isotopic separation of water pools in the soil (Brooks et al, 2010;Vargas et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Isotopic offsets between bulk plant water and its sources are larger in cool and wet environments

Casa

Barbeta

Rodríguez‐Uña

et al. 2022

Hydrol. Earth Syst. Sci.

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Abstract. Isotope-based approaches to study plant water sources rely on the assumption that root water uptake and within-plant water transport are non-fractionating processes. However, a growing number of studies have reported offsets between plant and source water stable isotope composition for a wide range of ecosystems. These isotopic offsets can result in the erroneous attribution of source water used by plants and potential overestimations of groundwater uptake by the vegetation. We conducted a global meta-analysis to quantify the magnitude of these plant source water isotopic offsets and explored whether their variability could be explained by either biotic or abiotic factors. Our database compiled 112 studies spanning arctic to tropical biomes that reported the dual water isotope composition (δ2H and δ18O) of plant (stem) and source water, including soil water (sampled following various methodologies and along a variable range of depths). We calculated plant source 2H offsets in two ways: a line conditioned excess (LC-excess) that describes the 2H deviation from the local meteoric water line and a soil water line conditioned excess (SW-excess) that describes the deviation from the soil water line, for each sampling campaign within each study. We tested for the effects of climate (air temperature and soil water content), soil class, and plant traits (growth form, leaf habit, wood density, and parenchyma fraction and mycorrhizal habit) on LC-excess and SW-excess. Globally, stem water was more depleted in 2H than in soil water (SW-excess < 0) by 3.02±0.65 ‰ (P < 0.05 according to estimates of our linear mixed model and weighted by sample size within studies). In 95 % of the cases where SW-excess was negative, LC-excess was negative, indicating that the uptake of water that had not undergone evaporative enrichment (such as groundwater) was unlikely to explain the observed soil–plant water isotopic offsets. Soil class and plant traits did not have any significant effect on SW-excess. SW-excess was more negative in cold and wet sites, whereas it was more positive in warm sites. The climatic effects on SW-excess suggest that methodological artefacts are unlikely to be the sole cause of observed isotopic offsets. Our results would imply that plant source water isotopic offsets may lead to inaccuracies when using the isotopic composition of bulk stem water as a proxy to infer plant water sources.

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Section: Introductionmentioning

confidence: 99%

Isotopic offsets between bulk plant water and its sources are larger in cool and wet environments

Casa

Barbeta

Rodríguez‐Uña

et al. 2022

Hydrol. Earth Syst. Sci.

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show abstract

“…Besides parameters inherent to the CVD protocol (mainly extraction time, temperature and vacuum line pressure), soil texture, cation exchange capacity and organic matter content have been shown to affect the isotopic composition of extracted soil water (Chen et al, 2021;Orlowski et al, 2018). Alternatives to CVD exist for soil samples, such as water 85 extraction with suction lysimeters (e.g Carrière et al, 2020) or online measurements of liquid-vapor equilibration (Dubbert et al, 2013), but CVD is still, by far, the most common methodology (Amin et al, 2020). The isotopic composition of stem water could also be altered following CVD, as hydrogen exchange between water and cellulose during extraction should cause a systematic, and potentially significant, depletion of the extracted water in 2 H (Chen et al, 2020).…”

Section: Introduction 35mentioning

confidence: 99%

Revealing a significant isotopic offset between plant water and its sources using a global meta-analysis

Casa

Barbeta

Rodríguez‐Uña

et al. 2021

Preprint

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Abstract. Isotope-based approaches to study plant water sources rely on the assumption that root water uptake and within-plant water transport are non-fractionating processes. However, a growing number of studies have reported offsets between plant and source water stable isotope composition, for a wide range of ecosystems. These isotopic offsets can result in the erroneous attribution of source water used by plants and potential overestimations of groundwater uptake by the vegetation. We conducted a global meta-analysis to quantify the magnitude of these plant-source water isotopic offsets and explore whether their variability could be explained by either biotic or abiotic factors. Our database compiled 112 studies, spanning arctic to tropical biomes that reported the dual water isotope composition (δ2H and δ18O) of plant (stem) and source water, including soil water. We calculated 2H offsets in two ways: a line conditioned excess (LC-excess) that describes the 2H deviation from the local meteoric water line, and a soil water line conditioned excess (SW-excess), that describes the deviation from the soil water line, for each sampling campaign within each study. We tested for the effects of climate (air temperature and soil water content), soil class and plant traits (growth form, leaf habit, wood density and parenchyma fraction and mycorrhizal habit) on LC-excess and SW-excess. Globally, stem water was more depleted in 2H than soil water (SW-excess < 0) by 3.02 ± 0.65 ‰. In 95 % of the cases where SW-excess was negative, LC-excess was negative, indicating that the uptake of water from mobile pools was unlikely to explain the observed soil-plant water isotopic offsets. SW-excess was more negative in cold and wet sites, whereas it was more positive in warm sites. Soil class and plant traits did not have any significant effect on SW-excess. The climatic effects on SW-excess suggest that methodological artefacts are unlikely to be the sole cause of observed isotopic offsets. Instead, our results support the idea that these offsets are caused by isotopic heterogeneity within plant stems whose relative importance will depend on soil and plant water status and evaporative demand. Our results would imply that plant-source water isotopic offsets may lead to inaccuracies when using the isotopic composition of bulk stem water as a proxy to infer plant water sources.

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“…The water from the xylem and soil was extracted using the cryogenic vacuum method described by Ehleringer et al (2000). Precipitation, karst spring, surface soil and xylem samples were analysed on a Los Gatos Isotope Ratio Infrared Spectrometer (IRIS, DLT‐100, Los Gatos Research, Mountain View, CA, USA) at the Institute of Subtropical Agroecology, Chinese Academy of Sciences, which is one of the methods used for accurate determination of water isotopes (Carrière et al, 2020b). Isotope analysis results were expressed in δ notation in per mil (‰) between the determined samples and Vienna Standard Mean Ocean Water (VSMOW).…”

Section: Methodsmentioning

confidence: 99%

“…Studies have shown that plants adopt a resource conservation strategy in semi-arid ecosystems to avoid dehydration by maximizing water capture below the root zone. This strategy can help plants survive water fluctuations and cope with extreme evaporative demands (Carrière et al, 2020b;Johnson et al, 2014;Levitt, 1985). Therefore, the SFSS in the epikarst zone changes the hydrological processes of karst hydrosystems and provides a water source for plant growth (McDonnell, 2003; Yan F I G U R E 1 Overview of the karst vadose zone main components (soil, epikarst and transfer zone) (Jouves et al, 2017;Poulain et al, 2018;modified and combined) et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…Jackson et al (1999), Ford and Williams (2013), Nie et al (2017) and Liu et al (2019) used isotopic tracing and showed that fissure water is a vital water source for plants. Carrière et al (2020b) combined isotopic tracing and leaf water potential to determine whether trees could take up water from the TZ. Although studies have recognized that plants can take up TZ water, the relative contributions of fissure and TZ water to plants are uncertain, and the mechanisms by which plants utilize fissure water require further study (Carrière et al, 2016; Kukowski et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Epikarst shallow fissure soil systems are key to eliminating karst drought limitations in the karst rocky desertification area of SW China

et al. 2021

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Surface soil water shortages are among the primary factors limiting revegetation in most degraded regions with shallow soil, especially in karst areas. Finding water sources for plants is an urgent task to ensure maximum vegetation restoration in these areas. We combined soil water content monitoring and isotope tracing to reveal the principal water source supply systems for plants in karst areas. The results showed that the content and storage of water in the shallow fissure soil system (SFSS) of the epikarst zone were consistently higher and more temporally stable than that of the surface soil. Thus, epikarsts with dissolution voids and fissures are important, stable aquifers that provide water to plants. Moreover, the IsoSource results showed that the SFSS was the primary water source for three monitored tree species (Cupressus torulosa D. Don, Pyracantha fortuneana [Maxim.] Li and Rosa cymosa Tratt.), especially C. torulosa and P. fortuneana. The water-uptake patterns of C. torulosa and P. fortuneana changed from dominant SFSS and surface soil water sources during the rainy period to the dominant SFSS and transfer zone (TZ) of vadose zone water sources during the dry period. In contrast, Rhynchospora cymosa uses water from SFSS and TZ water sources only during drought. These results suggest that the SFSS is key to eliminating vegetation restoration limitations due to surface drought in karst areas. It is proposed that deeply rooted plants with dimorphic root systems are optimal for sustainable vegetation restoration in karst areas.

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Tree xylem water isotope analysis by Isotope Ratio Mass Spectrometry and laser spectrometry: A dataset to explore tree response to drought

Cited by 9 publications

References 12 publications

Isotopic offsets between bulk plant water and its sources are larger in cool and wet environments

Isotopic offsets between bulk plant water and its sources are larger in cool and wet environments

Revealing a significant isotopic offset between plant water and its sources using a global meta-analysis

Epikarst shallow fissure soil systems are key to eliminating karst drought limitations in the karst rocky desertification area of SW China

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