Trehalose induces SQSTM1/p62 expression and enhances lysosomal activity and antioxidative capacity in adipocytes

Abstract

Trehalose is a disaccharide reported to ameliorate adipocyte hypertrophy and improve metabolic state. The present study demonstrated that trehalose augments the levels of SQSTM1, lysosome‐related factors and antioxidative factors, which induces lysosomal activity and antioxidative capacity in adipocytes. These observations reveal a mechanism for the above effects of trehalose.

Neuroprotective Effects of Trehalose and Sodium Butyrate on Preformed Fibrillar Form of α-Synuclein-Induced Rat Model of Parkinson’s Disease

“…SQSTM1 is a cytoplasmic protein detectable in inclusion bodies of protein aggregates that are responsible for degradation of ubiquitinated substrates. Here, we examined SQSTM1 levels in the SN region of the brain and observed faint bands in the PFF α-syn group which was reversed after tre and SB treatment, where the single treatment of the drugs increased SQSTM1 protein levels and the combination treatment significantly upregulated the SQSTM1 protein level when compared to the PFF α-syn group, which might be attributable to the capacity of tre to increase SQSTM1 levels . Moreover, when the combination group was compared with treatments alone, the SQSTM1 protein level was significantly increased, as * P ≤ 0.05 for tre+SB vs tre and * P ≤ 0.05 for tre+SB vs SB (Supplementary Figure 4A,B).…”

Activation of ectopic olfactory receptor 544 induces GLP-1 secretion and regulates gut inflammation

“… 51 Trehalose also induces antioxidative and lysosomal gene expression, resulting in reduced cellular reactive oxygen species in adipose tissues. 52 In addition, trehalose has been shown to activate hepatic Aloxe3, which results in the reduction of weight gain and hepatic steatosis, thus ameliorating metabolic disease. 53 These results collectively indicate that trehalose may have anti-obesogenic effects in multiple organs after uptake by the intestine.…”

Upconversion Nanoparticle-Based Cell Membrane-Coated cRGD Peptide Bioorthogonally Labeled Nanoplatform for Glioblastoma Treatment

“…Plasmids encoding green fluorescent protein (GFP) fused to LC3 (GFP-LC3) were obtained from Addgene (#21073, Watertown, MA). U87 cells were overexpressed GFP-LC3 using a retrovirus system using the pMXs-AMNN-puro-GFP-LC3 plasmid, as previously reported . In brief, the vectors were transfected into 293T cells according to the manufacturer’s protocol.…”

“…U87 cells were overexpressed GFP-LC3 using a retrovirus system using the pMXs-AMNN-puro-GFP-LC3 plasmid, as previously reported. 60 In brief, the vectors were transfected into 293T cells according to the manufacturer's protocol. Eight hours later, virus-containing culture supernatants were filtered with 0.22 μm filters (Millipore).…”