Trehalose: Metabolism and Role in Stress Signaling in Plants

John, Riffat; Raja, Vaseem; Ahmad, Mubashir; Jan, Nelofer; Majeed, Umer; Ahmad, Sujat; Yaqoob, Umer; Kaul, Tanushri

doi:10.1007/978-3-319-42183-4_11

Cited by 18 publications

(13 citation statements)

References 71 publications

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“…Trehalose is a double-face osmotic adjustment substance ( Fernandez et al, 2010 ; John et al, 2017 ), the reaction catalyzed by TPP is the final step in Tre biosynthesis in higher plants ( Paul et al, 2008 ; Fernandez et al, 2010 ). Luo et al (2010) have found that, in winter wheat seedlings, high temperature stress accumulated endogenous Tre, thus increasing the tolerance of seedlings to high temperature stress.…”

Section: Discussionmentioning

confidence: 99%

“…Antioxidant system includes antioxidant enzymes (superoxide dismutase: SOD, ascorbate peroxidase: APX, glutathione reductase: GR, catalase: CAT, and guaiacol peroxidase: GPX) and non-enzymatic antioxidants (ascorbic acid: AsA and glutathione: GSH), which can alleviate oxidative damage of biomacromolecules (proteins, lipids, DNA, and RNA) and biomembrane by scavenging excess ROS and MG; osmotic adjustment substances is also known as osmolytes or compatible solutes ( Ashraf and Foolad, 2007 ; Jha et al, 2014 ; Chaudhuri et al, 2017 ; John et al, 2017 ; Li et al, 2017a , b ). Osmotic adjustment substances have emerged multidimensional functions, implicating in osmotic adjustment (to combat with osmotic stress), redox buffering, and ROS scavenging (to ameliorate oxidative stress), and acting as small molecular chaperones (to stabilize the structures of protein, enzyme, DNA, RNA, and biomembrane) and development signaling molecule ( Chen and Murata, 2011 ; Ahmad et al, 2013 ; Chaudhuri et al, 2017 ; John et al, 2017 ). In higher plants, Δ 1 -pyrroline-5-carboxylate synthetase (P5CS), ornithine aminotransferase (OAT), betaine aldehyde dehydrogenase (BADH), and trehalose-6-phosphate phosphatase (TPP) are the rate-limiting enzymes in Pro, GB, and Tre biosynthesis, respectively.…”

Section: Introductionmentioning

confidence: 99%

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Signaling Molecule Hydrogen Sulfide Improves Seed Germination and Seedling Growth of Maize (Zea mays L.) Under High Temperature by Inducing Antioxidant System and Osmolyte Biosynthesis

Zhihao

Wang

et al. 2018

Front. Plant Sci.

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Hydrogen sulfide (H2S) is a novel type signaling molecule in plants. Seed germination is a key stage of life cycle of plants, which is vulnerable to environmental stress including high temperature. However, under high temperature stress, whether pre-soaking of maize seeds with NaHS (a H2S donor) could improve seed germination and seedling growth and the possible mechanisms are not completely clear. In this study, maize seeds pre-soaked with NaHS enhanced germination percentage, sprout length, root length, and fresh weight compared with the control without NaHS treatment, illustrating that H2S could improve maize seed germination and seedling growth under high temperature. In addition, in comparison to the control, NaHS pre-soaking stimulated antioxidant enzymes [ascorbate peroxidase (APX), glutathione reductase (GR), guaiacol peroxidase (GPX), superoxide dismutase (SOD), and catalase (CAT)] activities and the contents of water soluble non-enzymatic antioxidants [ascorbic acid (AsA) and glutathione (GSH)], as well as the ratio of reduced antioxidant to oxidized antioxidant. Moreover, pre-soaking with NaHS activated Δ1-pyrroline-5-carboxylate synthetase (P5CS) and ornithine aminotransferase [OAT; both are rate-limiting enzymes in proline (Pro) synthesis], betaine aldehyde dehydrogenase [BADH; a key enzyme in glycine betaine (GB)], and trehalose (Tre)-6-phosphate phosphatase (a key step in Tre synthesis), which in turn accumulated Pro, GB, and Tre in germinating seeds compared with the control. Also, an improved germination by NaHS under high temperature was reinforced by the above osmotic adjustment substances (osmolytes) alone, while deteriorated by the inhibitors of osmolyte biosynthesis [gabaculine (GAB), disulfiram (DSF), and sodium citrate (SC)]. These results imply that H2S could improve maize seed germination and seedling growth under high temperature by inducing antioxidant system and osmolyte biosynthesis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Signaling Molecule Hydrogen Sulfide Improves Seed Germination and Seedling Growth of Maize (Zea mays L.) Under High Temperature by Inducing Antioxidant System and Osmolyte Biosynthesis

Zhihao

Wang

et al. 2018

Front. Plant Sci.

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“…Trehalose 6-phosphate (Tre6P), an intermediate of trehalose biosynthesis, is of paramount importance as a signal metabolite in plants, connecting growth and development to carbon status (Figueroa and Lunn, 2016). Tre is responsible for expression of genes and signaling pathways associated with stress response and detoxification (Abdallah et al 2016;John et al 2017). Trehalose possesses high hydrophilicity because of the absence of internal hydrogen bonding (Paul and Paul 2014;Abdallah et al 2016).…”

Section: Sugars: Trehalosementioning

confidence: 99%

Osmoprotection in plants under abiotic stresses: new insights into a classical phenomenon

Zulfiqar

Akram

Ashraf

2019

Planta

242

112

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“…Trehalose is known to be involved in stress responses, particularly as an osmotic protectant and as a nutrient reserve in plants [45]. Mut68 contains greatly reduced trehalose contents, which can render it imbalanced in stress-related amino acids.…”

Section: Further Metabolite Profiling Of Amino Acidsmentioning

confidence: 99%

Development and characterization of a Nannochloropsis mutant with simultaneously enhanced growth and lipid production

Ryu

Kang

Jeon

et al. 2020

Biotechnol Biofuels

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Background: The necessity to develop high lipid-producing microalgae is emphasized for the commercialization of microalgal biomass, which is environmentally friendly and sustainable. Nannochloropsis are one of the best industrial microalgae and have been widely studied for their lipids, including high-value polyunsaturated fatty acids (PUFAs). Many reports on the genetic and biological engineering of Nannochloropsis to improve their growth and lipid contents have been published. Results: We performed insertional mutagenesis in Nannochloropsis salina, and screened mutants with high lipid contents using fluorescence-activated cell sorting (FACS). We isolated a mutant, Mut68, which showed improved growth and a concomitant increase in lipid contents. Mut68 exhibited 53% faster growth rate and 34% higher fatty acid methyl ester (FAME) contents after incubation for 8 days, resulting in a 75% increase in FAME productivity compared to that in the wild type (WT). By sequencing the whole genome, we identified the disrupted gene in Mut68 that encoded trehalose-6-phosphate (T6P) synthase (TPS). TPS is composed of two domains: TPS domain and T6P phosphatase (TPP) domain, which catalyze the initial formation of T6P and dephosphorylation to trehalose, respectively. Mut68 was disrupted at the TPP domain in the C-terminal half, which was confirmed by metabolic analyses revealing a great reduction in the trehalose content in Mut68. Consistent with the unaffected N-terminal TPS domain, Mut68 showed moderate increase in T6P that is known for regulation of sugar metabolism, growth, and lipid biosynthesis. Interestingly, the metabolic analyses also revealed a significant increase in stress-related amino acids, including proline and glutamine, which may further contribute to the Mut68 phenotypes. Conclusion: We have successfully isolated an insertional mutant showing improved growth and lipid production. Moreover, we identified the disrupted gene encoding TPS. Consistent with the disrupted TPP domain, metabolic analyses revealed a moderate increase in T6P and greatly reduced trehalose. Herein, we provide an excellent proof of concept that the selection of insertional mutations via FACS can be employed for the isolation of mutants with

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Trehalose: Metabolism and Role in Stress Signaling in Plants

Cited by 18 publications

References 71 publications

Signaling Molecule Hydrogen Sulfide Improves Seed Germination and Seedling Growth of Maize (Zea mays L.) Under High Temperature by Inducing Antioxidant System and Osmolyte Biosynthesis

Signaling Molecule Hydrogen Sulfide Improves Seed Germination and Seedling Growth of Maize (Zea mays L.) Under High Temperature by Inducing Antioxidant System and Osmolyte Biosynthesis

Osmoprotection in plants under abiotic stresses: new insights into a classical phenomenon

Development and characterization of a Nannochloropsis mutant with simultaneously enhanced growth and lipid production

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