Tremella fuciformis polysaccharide suppresses hydrogen peroxide-triggered injury of human skin fibroblasts via upregulation of SIRT1

Abstract

Abstract. Tremella fuciformis polysaccharide (TFPS), which is the extract of Tremella fuciformis Berk, has previously been demonstrated to exhibit potent anti-oxidative, anti-inflammatory and anti-aging effects. However, the mechanisms underlying these protective and therapeutic effects remain to be elucidated. The aim of the present study was to investigate the protective effects of TFPS on hydrogen peroxide-induced injury of human skin fibroblasts and to elucidate the aforementioned underlying mechanisms. A hydrogen peroxide-induced human skin fibroblast injury model was firstly established. MTT and reactive oxygen species (ROS) production assays, in addition to terminal deoxynucleotidyl transferase dUTP nick end labeling, reverse transcription-quantitative polymerase chain reaction and western blotting, were performed to investigate the protective effects of TFPS. Hydrogen peroxide decreased human skin fibroblast viability with a concurrent increase in ROS generation and cell apoptosis. Treatment with 0-400 µg/ml TFPS alone for up to 48 h did not result in alteration in cell viability. Notably, TFPS pre-treatment reduced oxidative stress and cell apoptosis in hydrogen peroxide-treated skin fibroblasts. In addition, there was profound inhibition of p16, p21, p53 and caspase-3 expression, and activation of extracellular-signal regulated kinase and Akt serine/threonine kinase 1, following TFPS pre-treatment. Furthermore, it was revealed that TFPS additionally protected fibroblasts via the upregulation of SIRT1 expression, and this was abrogated by the SIRT1 inhibitor niacinamide. These results indicated that TFPS alleviated hydrogen peroxide-induced oxidative stress and apoptosis in skin fibroblasts via upregulation of SIRT1 expression, indicating that TFPS may act as a potential therapeutic agent for oxidative-stress-associated skin diseases and aging. IntroductionOxidative stress induced by free radicals, which are produced in response to redox reactions, radiation and chemical reactions, can cause DNA and protein damage. Such stress is associated with the development of many diseases, such as atherosclerosis, diabetes mellitus, articular gout, and cancer (1,2). Hydrogen peroxide (H 2 O 2 ) is one of type of reactive oxygen species (ROS) produced in normal cells during cell respiration and metabolism (3). However, many studies have indicated that a high concentration of H 2 O 2 , present under pathological conditions, may induce various human degenerative diseases and aging. Furthermore, H 2 O 2 also interacts with intracellular ions such as iron and copper, leading to a chain reaction that produces more reactive radicals and amplifies the damage, thereby causing DNA and protein damage (4). Therefore, antioxidants are very important for the treatment of oxidative-stress-related diseases and aging.Antioxidants in food, including vitamin C, polyphenols, tocopherols and f lavonoids, may prevent free-radical-induced cell damage (5). They also may attenuate oxidative-stress-induced inflammatory reactions and apopto...

Tremella fuciformis Polysaccharides Attenuate Oxidative Stress and Inflammation in Macrophages through miR-155

“…The supernatant was precipitated with 98% ethanol, deproteinized by the Sevag method, purified through a dialysis membrane, and freeze-dried to obtain pure TFPS. Under the above conditions, the yield of Tremella fuciformis polysaccharide reaches 20.52% while the yield obtained by quantitative analysis is 20.25%, as reported previously [ 14 ].…”

“…Tremella fuciformis polysaccharides (TFPS), which are extracted from edible mushrooms, exhibit numerous biological functions, including immunomodulatory, antineoplastic, and hypoglycemic effects [ 7 , 14 , 18 ]. In our study, we measured the inflammation and oxidative stress of macrophages in response to TFPS.…”

Antioxidant action and protective and reparative effects of lentinan on oxidative damage in HaCaT cells

“…To better reflect the actual metabolism of the body, we further employed a cellular model of oxidative damage to determine the antioxidant potential of lentinan. H 2 O 2 has long been used widely to establish cellular models of acute oxidative stress, for investigating free‐radical‐induced damage to cells . The experiments were conducted with the HaCaT cell line, derived from the outermost layers of human epidermis, and established an H 2 O 2 ‐induced cellular damage model .…”

Preparation, characterization, and cytokine‐stimulating activity of oligosaccharides from Tremella fuciformis Berk

“…In addition, its polysaccharide extract, called “Yiner Capsule,” is clinically applied as a traditional drug for the treatment of patients with tumors in China. According to the literature, Tremella polysaccharides (TP) have several pharmacological activities, such as immunomodulatory (Du et al, 2009; Jiang et al, 2012; Ruan, Li, Pu, Shen, & Jin, 2018; Zhao, Hu, Wang, Liu, & Guo, 2013), anti‐tumor (Chen, 2010; Cho et al, 2007), hypoglycaemic activity (Bach et al, 2015; Wang, Zhang, & Zhao, 2015), antioxidant (Wen et al, 2016), anti‐radiation (Xu et al, 2012), anti‐aging (Shen et al, 2017), anti‐inflammatory (Li, Lee, Kim, Moon, & Lee, 2014), neuroprotective effect (Jin, Hu, Zhang, & Liu, 2016), and so on. Due to the beneficial activities and safety of TP, some studies have focused on elucidating the relationship between the structure and activities of TP (Jiang et al, 2012; Slodki, 1966).…”