Trends and Challenges in Pesticide Resistance Detection

Barrès, Benoît; Micoud, Annie; Corio‐Costet, Marie‐France; Debieu, Danièle; Fillinger, Sabine; Walker, Anne‐Sophie; Délye, Christophe; Grosman, Jacques; Siegwart, Myriam

doi:10.1016/j.tplants.2016.06.006

Cited by 87 publications

(30 citation statements)

References 162 publications

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“…The two main mechanisms predominantly responsible for insecticide resistance are target site insensitivity and metabolic resistance due to elevated levels of insecticide detoxifying enzymes ( Li et al, 2007 ; Liu, 2015 ; Barres et al, 2016 ). Biochemical assays have demonstrated the resistance to carbamates and organophosphates caused by insensitive acetylcholinesterase (ACE) in a lot of insect species ( Hemingway et al, 2004 ; Bass et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Functional Analysis of a Carboxylesterase Gene Associated With Isoprocarb and Cyhalothrin Resistance in Rhopalosiphum padi (L.)

Wang

Huang

et al. 2018

Front. Physiol.

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Carboxylesterase (CarE) is an important class of detoxification enzymes involved in insecticide resistance. However, the molecular mechanism of CarE-mediated insecticide resistance in Rhopalosiphum padi, a problematic agricultural pest, remains largely unknown. In the present study, an isoprocarb-resistant (IS-R) strain and a cyhalothrin-resistant (CY-R) strain were successively selected from a susceptible (SS) strain of R. padi. The enzyme activity indicated that enhanced carboxylesterase activity contributes to isoprocarb and cyhalothrin resistance. The expression levels of putative CarE genes were examined and compared among IS-R, CY-R, and SS strains, and only the R. padi carboxylesterase gene (RpCarE) was significantly over expressed in both the IS-R and CY-R strains compared to the SS strain. The coding region of the RpCarE gene was cloned and expressed in Escherichia coli. The purified RpCarE protein was able to catalyze the model substrate, α-naphtyl acetate (Kcat = 5.50 s-1; Km = 42.98 μM). HPLC assay showed that the recombinant protein had hydrolase activity against isoprocarb and cyhalothrin. The modeling and docking analyses consistently indicated these two insecticide molecules fit snugly into the catalytic pocket of RpCarE. Taken together, these findings suggest that RpCarE plays an important role in metabolic resistance to carbamates and pyrethroids in R. padi.

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Section: Introductionmentioning

confidence: 99%

Functional Analysis of a Carboxylesterase Gene Associated With Isoprocarb and Cyhalothrin Resistance in Rhopalosiphum padi (L.)

Wang

Huang

et al. 2018

Front. Physiol.

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“…In addition, they can also be used to purposefully regulate, control, and affect the metabolism, growth, development, and reproduction of plants [ 20 , 21 ]. However, serious issues of food safety and environmental pollution have attracted more and more attention due to the misuse of these toxic residue pesticides [ 22 , 23 ]. Hence, it is of great significance for the detection of pesticides.…”

Section: Introductionmentioning

confidence: 99%

Spectral Characterization and Molecular Dynamics Simulation of Pesticides Based on Terahertz Time-Domain Spectra Analyses and Density Functional Theory (DFT) Calculations

Lin

et al. 2018

Molecules

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This work provides the experimental and theoretical fundamentals for detecting the molecular fingerprints of six kinds of pesticides by using terahertz (THz) time-domain spectroscopy (THz-TDS). The spectra of absorption coefficient and refractive index of the pesticides, chlorpyrifos, fipronil, carbofuran, dimethoate, methomyl, and thidiazuron are obtained in frequencies of 0.1–3.5 THz. To accurately describe the THz spectral characteristics of pesticides, the wavelet threshold de-noising (WTD) method with db 5 wavelet fucntion, 5-layer decomposition, and soft-threshold de-noising was used to eliminate the spectral noise. The spectral baseline correction (SBC) method based on asymmetric least squares smoothing was used to remove the baseline drift. Spectral results show that chlorpyrifo had three characteristic absorption peaks at 1.47, 1.93, and 2.73 THz. Fipronil showed three peaks at 0.76, 1.23, and 2.31 THz. Carbofuran showed two peaks at 2.72 and 3.06 THz. Dimethoate showed three peaks at 1.05, 1.89, and 2.92 THz. Methomyl showed five peaks at 1.01, 1.65, 1.91, 2.72, and 3.20 THz. Thidiazuron showed four peaks at 0.99, 1.57, 2.17, and 2.66 THz. The density functional theory (DFT) of B3LYP/6-31G+(d,p) was applied to simulate the molecular dynamics for peak analyzing of the pesticides based on isolated molecules. The theoretical spectra are in good agreement with the experimental spectra processed by WTD + SBC, which implies the validity of WTD + SBC spectral processing methods and the accuracy of DFT spectral peak analysis. These results support that the combination of THz-TDS and DFT is an effective tool for pesticide fingerprint analysis and the molecular dynamics simulations.

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“…By the turn of the twenty-irst century, this atitude began to turn around as, for example, 'super bugs' invading humans have developed antibiotic resistance, and plant pathogens have likewise developed resistance to pesticides [65][66][67][68]. Reductionism in modern science is proving to be less helpful in facing new challenges because it compartmentalizes complex topics and ignores TEK, when there is considerable urgency and essentiality in supporting 'the integration of methods and results from diferent approaches and levels of analysis' [69, p. 466].…”

Section: From Quackgrass To Quackery and Backmentioning

confidence: 99%

Beyond Turf and Lawn: Poaceae in This Age of Climate Change

Dunster¹

2017

Grasses - Benefits, Diversities and Functional Roles

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Grassland ecosystems dominated by Poaceae are estimated to cover 40.5% of Earth's land base, and domestication of a few edible grass species into highly productive cereal grains aided the shift from nomadic food gathering to ield cultivation and higher density habitation. In the Northern Hemisphere, grasses are used ornamentally and for pasture, fodder and forage with litle thought that the grasses livestock grazed upon, or those we gaze upon have multi-functional uses elsewhere. In this age of climate change, the use of Poaceae solely for aesthetics and amenity depletes inite potable water supplies that are needed for human survival. Agricultural land is consumed for turf seed production, and land is removed from food production. Cereals and their growing regions, which we have depended upon as food for millennia, are unlikely to adapt to climate change, and this will result in food insecurity and famines. Despite traditional uses of Poaceae in various cultures, many unrealized needs for food, medicine and other material goods could be met elsewhere with knowledge transfer. Our modern relationship with grass as an ornamental or amenity must end. As designers, gardeners and urban dwellers, we must use Poaceae for its multi-functions, which will lead to resilience and survival.

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Trends and Challenges in Pesticide Resistance Detection

Cited by 87 publications

References 162 publications

Functional Analysis of a Carboxylesterase Gene Associated With Isoprocarb and Cyhalothrin Resistance in Rhopalosiphum padi (L.)

Functional Analysis of a Carboxylesterase Gene Associated With Isoprocarb and Cyhalothrin Resistance in Rhopalosiphum padi (L.)

Spectral Characterization and Molecular Dynamics Simulation of Pesticides Based on Terahertz Time-Domain Spectra Analyses and Density Functional Theory (DFT) Calculations

Beyond Turf and Lawn: Poaceae in This Age of Climate Change

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