Trends in phage types of Salmonella enterica serovars Enteritidis and Typhimurium isolated in Slovakia from 1995 to 2009

Majtánová, L; Majtán, Juraj; Majtán, V.

doi:10.1016/j.diagmicrobio.2010.10.017

Cited by 11 publications

(10 citation statements)

References 13 publications

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“…However, DT193 S . Typhimurium strains (as well as DT204, DT204c and DT29) were responsible for human gastroenteritis-outbreaks (with bovine reservoirs) in the United Kingdom in the 1970s and DT193 is one of the most common long term phage types identified from clinical isolates in Slovakia [34,35,36]. DT193 is also a phage type of great concern in Brazil where its detection amongst clinical isolates in S .…”

Section: Resultsmentioning

confidence: 99%

Resistance Genes, Phage Types and Pulsed Field Gel Electrophoresis Pulsotypes in Salmonella enterica Strains from Laying Hen Farms in Southern Italy

Camarda

Pugliese

Pupillo

et al. 2013

IJERPH

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Twenty-four Salmonella enterica isolates (13 serovar Enteritidis and 11 Typhimurium) isolated from 5,600 samples from intensive laying hen farms in Italy in 1998–2007 were characterized for antimicrobial resistance genes, pulsotype and phage type. Most of S. Typhimurium strains were pulsotype STYMXB.0147 (81.8%), phage type DT143 and resistant to sulfamethoxazole encoded by sul2. Two multidrug resistant (MDR) strains were identified. One strain, STYMXB.0061, was resistant to ampicillin (A), chloramphenicol (C), streptomycin (S), sulfamethoxazole (Su) and tetracycline (T) encoded by the Salmonella Genomic Island SGI1. The second MDR strain, STYMXB.0110, was resistant to SSuT encoded by sul1 and sul2, aadA1 and tet(C)-flanked by an IS26 element, respectively. The tet(C) gene has been reported to confer low levels of resistance and it has very rarely been detected in S. Typhimurium from poultry. In the current study, the MIC value (32 µg/mL) was consistent with the breakpoint (≥16 µg/mL) reported for Enterobacteriaceae. Most of the S. Enteritidis strains were resistant to Su (encoded by sul2). One MDR strain (ANxSSuT) was identified. With the exception of nalidixic acid (Nx), the resistances were respectively encoded by blaTEM, strAB, sul2 and tet(A) harbored by an IncN conjugative plasmid. All isolates were pulsotype SENTXB.0001 with PT14b being the most prevalent identified phage type (57.1%). In Europe, SENTXB.0001 is the predominant PFGE profile from clinical cases and the identification of PT14b has steadily been on the increase since 2001. The findings presented in this study highlight the potential spread of S. Enteritidis phage types PT14b and S. Typhimurium DT143 in a field of particular relevance for zoonoses. Additional, the presence of resistance genes and genetic elements (conjugative plasmid and IS element) underlines the need to assess routinely studies in field, such as poultry farms, relevant fot the public health and suitable for the storage and diffusion of antimicrobial resistance.

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Section: Resultsmentioning

confidence: 99%

Resistance Genes, Phage Types and Pulsed Field Gel Electrophoresis Pulsotypes in Salmonella enterica Strains from Laying Hen Farms in Southern Italy

Camarda

Pugliese

Pupillo

et al. 2013

IJERPH

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“…The major advantage of PT is that it is a globally accepted method and specific phage type numbers can be assigned to isolates, which makes comparison between isolates possible on a worldwide scale. In addition, PT has good intralaboratory reproducibility (Majtanova et al, 2011). However, some strains are non-typeable, and possible phage type conversion (Brown et al, 1999;Chart et al, 1989;TankouoSandjong et al, 2012;Threlfall et al, 1989) can occur within the serotype.…”

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confidence: 99%

“…Some phage types can also predominate in a geographical area, which can limit the utility of PT for investigating local outbreaks (Lukinmaa et al, 1999). Another disadvantage is that only a limited number of reference laboratories perform PT (Cho et al, 2008;Majtanova et al, 2011). Pulsed-field gel electrophoresis (PFGE) using XbaI is another standard method for genotyping SE (Laconcha et al, 2000;Rivoal et al, 2009).…”

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confidence: 99%

Molecular Characterization ofSalmonellaEnteritidis: Comparison of an Optimized Multi-Locus Variable-Number of Tandem Repeat Analysis (MLVA) and Pulsed-Field Gel Electrophoresis

Dewaele¹,

Rasschaert²,

Bertrand³

et al. 2012

Foodborne Pathogens and Disease

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Salmonella Enteritidis (SE) is a genetically homogenous serovar, which makes optimal subtype discrimination crucial for epidemiological research. This study describes the development and evaluation of an optimized multiple-locus variable number tandem-repeat assay (MLVA) for characterization of SE. The typeability and discriminatory power of this MLVA was determined on a selected collection of 60 SE isolates and compared with pulsed-field gel electrophoresis (PFGE) using restriction enzymes XbaI, NotI, or SfiI. In addition, the estimated Wallace coefficient (W) was calculated to assess the congruence of the typing methods. Selection of epidemiologically unrelated isolates and more related isolates (originating from layer farms) was also based on the given phage type (PT). When targeting six loci, MLVA generated 16 profiles, while PFGE produced 10, 9, and 16 pulsotypes using XbaI, NotI, and SfiI, respectively, for the entire strain collection. For the epidemiologically unrelated isolates, MLVA had the highest discriminatory power and showed good discrimination between isolates from different layer farms and among isolates from the same layer farm. MLVA performed together with PT showed higher discriminatory power compared to PFGE using one restriction enzyme together with PT. Results showed that combining PT with the optimized MLVA presented here provides a rapid typing tool with good discriminatory power for characterizing SE isolates of various origins and isolates originating from the same layer farm.

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“…The MLVA is an upcoming molecular typing method with excellent discriminatory power, practical performance, and ease of analysis and interpretation of results (Beranek et al, 2009;Gho et al, 2010). Phage typing has been traditionally used for surveillance of common Salmonella serovars in humans, food, and food-producing animals (van Duijkeren et al, 2002;Gollard et al, 2008;Majtanova et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Polyphasic characterization of Salmonella Enteritidis isolates on persistently contaminated layer farms during the implementation of a national control program with obligatory vaccination: A longitudinal study

Dewaele¹,

Rasschaert²,

Wildemauwe

et al. 2012

Poultry Science

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Since 2007, a national Salmonella control program including obligatory vaccination has been ongoing in Belgium. In this context, the aim of the present study was to investigate the diversity of Salmonella enterica serovar Enteritidis isolates on 5 persistently contaminated Belgian layer farms and to examine the potential sources and transmission routes of Salmonella Enteritidis contamination on the farms during successive laying rounds. A collection of 346 Salmonella isolates originating from the sampled farms were characterized using a combination of multilocus variable number of tandem repeat analysis (MLVA) and phage typing (PT). On each farm, one or 2 dominant MLVA-PT types were found during successive laying cycles. The dominant MLVA type was different for each of the individual farms, but some farms shared the same dominant phage type. Isolates recovered from hens' feces and ceca, egg contents, eggshells, vermin (mice, rats, red mites, and flies), and pets (dog and cat feces) had the same MLVA-PT type also found in the inside henhouse environment of the respective layer farm. Persistent types were identified in the layer farm inside environment (henhouse and egg collecting area). Furthermore, this study demonstrated cross-contamination of Salmonella between henhouses and between the henhouse and the egg collecting area. Additional isolates with a different MLVA-PT type were also recovered, mainly from the egg collecting area. A potential risk for cross-contamination of Salmonella between the individual layer farms and their egg trader was identified.

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Trends in phage types of Salmonella enterica serovars Enteritidis and Typhimurium isolated in Slovakia from 1995 to 2009

Cited by 11 publications

References 13 publications

Resistance Genes, Phage Types and Pulsed Field Gel Electrophoresis Pulsotypes in Salmonella enterica Strains from Laying Hen Farms in Southern Italy

Resistance Genes, Phage Types and Pulsed Field Gel Electrophoresis Pulsotypes in Salmonella enterica Strains from Laying Hen Farms in Southern Italy

Molecular Characterization ofSalmonellaEnteritidis: Comparison of an Optimized Multi-Locus Variable-Number of Tandem Repeat Analysis (MLVA) and Pulsed-Field Gel Electrophoresis

Polyphasic characterization of Salmonella Enteritidis isolates on persistently contaminated layer farms during the implementation of a national control program with obligatory vaccination: A longitudinal study

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