Pectin is a complex polysaccharide and has been used in food and pharmaceutical industries. The current study aimed to determine antioxidant and cytotoxic activities of pectin from peels of grape fruit (GP) and lemon (LP) by ferric reducing power (FRP) assay and brine shrimp lethality bioassay respectively. In addition, antioxidant and cytotoxic activities of GP and LP were compared with food-grade pectin (FGP). GP and LP exhibited positive results of antioxidant and cytotoxic activities. The percentage FRP of GP at 10 to 100 µg/ml was found to be higher as compared to FGP while LP showed closed values to that of FGP. The observed percentage mortality of shrimps at 10 to 1000 µg/ml by LP was found to be higher as compared to percentage mortality caused by FGP. Percentage mortality of shrimps by GP and FGP showed closed values. The LC 50 of LP was found to be lower than that of FGP but close to the LC 50 of standard drug etoposide. The LC 50 of GP was closed to that of FGP and higher than that of etoposide. The obtained results suggested costeffective use of GP and LP for antioxidant and cytotoxic actions. INTRODUCTION: The word pectin is originated from the Greek language which means curdled or congealed. Pectin is present in the primary cell wall and middle lamella of higher plants 1. Chemically, structure of pectin belongs to heteropolysaccharide that is consisted of D-galacturonic acid, Lrhamnose, L-arabinose and D-galactose linked by 1, 4 glycosidic linkages 2. Continuously increased production of free radicals in the body can cause mutation in DNA which can lead to production of cancer cells 3 .