We present a unique proxy for the reconstruction of variation in precipitation over the Amazon: oxygen isotope ratios in annual rings in tropical cedar (Cedrela odorata). A century-long record from northern Bolivia shows that tree rings preserve the signal of oxygen isotopes in precipitation during the wet season, with weaker influences of temperature and vapor pressure. Tree ring δ 18 O correlates strongly with δ 18 O in precipitation from distant stations in the center and west of the basin, and with Andean ice core δ 18 O showing that the signal is coherent over large areas. The signal correlates most strongly with basin-wide precipitation and Amazon river discharge. We attribute the strength of this (negative) correlation mainly to the cumulative rainout processes of oxygen isotopes (Rayleigh distillation) in air parcels during westward transport across the basin. We further find a clear signature of the El Niño-Southern Oscillation (ENSO) in the record, with strong ENSO influences over recent decades, but weaker influence from 1925 to 1975 indicating decadal scale variation in the controls on the hydrological cycle. The record exhibits a significant increase in δ 18 O over the 20th century consistent with increases in Andean δ 18 O ice core and lake records, which we tentatively attribute to increased water vapor transport into the basin. Taking these data together, our record reveals a fresh path to diagnose and improve our understanding of variation and trends of the hydrological cycle of the world's largest river catchment.climate change | dendrochronology | plant physiology T he Amazon basin is a major center of atmospheric convection and precipitation (1), and at the same time the world's largest drainage basin. The Amazon's river discharge accounts for ∼17% of the annual global discharge to the oceans (2); its hydrological cycle is tightly linked with the carbon cycle of the Amazon rainforest (3), which itself is one of the largest terrestrial biomass carbon pools. Changes in the hydrological cycle of the Amazon may therefore significantly affect atmospheric dynamics and global climate by changing atmospheric CO 2 concentration. Two particularly severe droughts occurred within the last decade (4), and long-term river records of the Amazon show a steady increase in discharge over the last century (5), indicating that the system may start to undergo some changes. It has been suggested that these changes are a result of anthropogenic warming, but they may also be part of longer-term, natural climatic variability. To clarify such a globally important issue, long-term, accurate records of the hydrological cycle of the Amazon basin are needed. However, meteorological data are only reliable for the last 50-60 y (6), and annually resolved long-term proxies for the hydrological cycle from within the Amazon basin itself are very scarce (7).A commonly used diagnostic for strength and functioning of the water cycle is the isotopic composition of precipitation. Although variation of oxygen isotope ratios in precip...