Triacylglycerols composition analysis of olive oils by ultra‐performance convergence chromatography combined with quadrupole time‐of‐flight mass spectrometry

Abstract

Summary Triacylglycerols (TAGs) compositions of ten commercial olive oil samples were analysed using an ultra‐performance convergence chromatography (UPC2) combined with a quadrupole time‐of‐flight mass spectrometry (Q‐TOF MS). A total of twenty‐three TAGs were characterised based on their Q‐TOF MS accurate molecular weight and MS2 fragment ion profiles, and seventeen of those were reported in olive oils for the first time. O‐O‐O was the most abundant TAG in all tested olive oil samples, followed by P‐O‐O, O‐O‐S and O‐O‐L. G‐G‐B had the lowest relative concentration in all the TAGs, ranging 0.02–0.08% of the total sample weight. The results from this study are important for further understanding the nutritional value and stability of olive or other edible oils.

Triacylglycerols and Fatty Acid Compositions of Cucumber, Tomato, Pumpkin, and Carrot Seed Oils by Ultra-Performance Convergence Chromatography Combined with Quadrupole Time-of-Flight Mass Spectrometry

“…The MS 2 daughter ion of diacyl fragments in peak 23 were m/z 603.5345 and 601.5236 ([M-RCOO+H] + ) ( Figure 3 b), representing the fragments of [O-O] + and [O-L] + with relative natural abundances of 41% and 100%, respectively. It has been previously proved that fatty acids on sn-2 position binding to TAGs have greater bond energy and are harder to be eliminated in MS 2 collision energy mode than those on sn-1 and sn-3 positions [ 20 , 21 , 22 , 24 , 25 ]. The ratio of [O-L] + and [O-O] + was over 2:1, suggesting that peak 23 could be tentatively identified as O-L-O.…”

“…The conditions of UPC 2 were set according to our previous manuscript [22]. A Waters Acquity ultra-performance convergence chromatography (UPC 2 ) system (Milford, MA, USA) equipped with a binary pump, an oven, an auto-sampler, a back-pressure regulator, and an Acquity UPC 2 BEH HSS C 18 column (150 mm × 3.0 mm i.d.…”

“…Representative base peak intensity (BPI) chromatograms of cucumber, tomato, pumpkin, and carrot seed oils obtained by UPC 2 -Q-TOF-MS are presented in Figure 1. The TAG compositions in the seed oils were determined using the accurate mass data of quasi-molecular ions of [M+NH 4 ] + and MS 2 fragmentation ions of [M+H] + information [21,22]. There are three typical types of TAGs: TAG that contain 3 same fatty acid side chains; or 3 different fatty acid side chains; or 2 same and 1 different fatty acid side chain with the different one at sn-2 or sn-1/sn-3 position.…”

“…Besides, the high-resolution quadrupole time-of-flight mass spectrometry (Q-TOF MS) system was able to provide accurate molecular mass weight and fragment ion information of TAGs and therefore played an important role in identifying the binding position of fatty acid side chains. The combination of UPC 2 and Q-TOF MS technology has been used to examine TAGs compositions of cow milk fat [ 20 ], sunflower, corn, soybean oils [ 21 ], and olive oils [ 22 ] due to the excellent resolution and separation ability.…”

Triacylglycerol and Fatty Acid Compositions of Blackberry, Red Raspberry, Black Raspberry, Blueberry and Cranberry Seed Oils by Ultra-Performance Convergence Chromatography-Quadrupole Time-of-Flight Mass Spectrometry

“…All the five seed oil samples were prepared based on our previous published methods [26][27][28][29]. One aliquot of 10 µL of each berry seed oil sample was mixed with 990 µL of acetonitrile/methanol/isopropanol (10:9:1, v/v/v), vortex-mixed for 20 s and centrifuged at 2000 rpm for 5 min at an ambient temperature.…”