Triazine Dyes Inhibit HIV‐1 Entry by Binding to Envelope Glycoproteins

Hattori, Toshio; Zhang, Xiaoyan; Weiss, Carol D.; Xu, Yao; Kubo, Toru; Sato, Yoshimi; Nishikawa, Shigemichi; Sakaida, Hitoshi; Uchiyama, Takashi

doi:10.1111/j.1348-0421.1997.tb01916.x

Cited by 14 publications

(4 citation statements)

References 28 publications

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“…The sepharose blue column was able to remove some of the albumin from the cell culture and did not bind the recombinant proteins. This is different from what was seen by Hattori et al in 1997 where gp120 was isolated using triazine dyes.…”

Section: Resultscontrasting

confidence: 55%

Limited cross-reactivity between different HIV-1 clades

Mthunzi¹,

Meyer²

2004

Journal of Clinical Virology

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Section: Resultscontrasting

confidence: 55%

Limited cross-reactivity between different HIV-1 clades

Mthunzi¹,

Meyer²

2004

Journal of Clinical Virology

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“…This triazine-derived textile dye has a negative charge and has commonly been used for protein purification via affinity chromatography. 24 The structure of the triazine dyes mimics the structure of substrates that bind to the active sites of different proteins, and is therefore able to bind a broad class of proteins and enzymes. 25 It is possible that triazine dyes may bind to molecules involved in the infection mediated by viral envelope proteins, or more specifically, the extracellular portion of viral glycoproteins that is involved in virus-cell fusion.…”

Section: Discussionmentioning

confidence: 99%

Enhanced detection of respiratory pathogens with nanotrap particles

et al. 2016

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The Influenza virus is a leading cause of respiratory disease in the United States each year. While the virus normally causes mild to moderate disease, hospitalization and death can occur in many cases. There are several methodologies that are used for detection; however problems such as decreased sensitivity and high rates of false-negative results may arise. There is a crucial need for an effective sample preparation technology that concentrates viruses at low abundance while excluding resident analytes that may interfere with detection. Nanotrap particles are hydrogel particles that are coupled to chemical dye affinity baits that bind a broad range of proteins and virions. Within minutes (<30 minutes), Nanotrap particles concentrate low abundant proteins and viruses from clinically complex matrices. Nanotrap particles with reactive red baits concentrated numerous respiratory viruses including various strains and subtypes of Influenza virus, Coronavirus, and Respiratory Syncytial Virus from saliva, nasal fluid swab specimens, and nasal aspirates. Detection was enhanced more than 10-fold when coupled to plaque assays and qRT-PCR. Importantly, Nanotrap particle can efficiently capture and concentrate multiple viral pathogens during a coinfection scenario. These results collectively demonstrate that Nanotrap particles are an important tool that can easily be integrated into various detection methodologies.

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“…They have been found eective against Gram-positive bacteria, fungi, parasites, viruses and coccidia (Hattori et al 1997;McFarland 1992;Nagamatsu et al 1993;Schmahl et al 1992). They have been found eective against Gram-positive bacteria, fungi, parasites, viruses and coccidia (Hattori et al 1997;McFarland 1992;Nagamatsu et al 1993;Schmahl et al 1992).…”

Section: Introductionmentioning

confidence: 97%

Triazinyl derivatives that are potent inhibitors of glucose transport in Trypanosoma brucei

Bayele

2001

Parasitol Res

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Glucose transport in Trypanosoma brucei is facilitated by a transporter that is kinetically markedly dierent from its mammalian homologue. In this regard, the trypanosomal transporter may be selectively targeted. We investigated the potential of a series of triazinyl derivatives as inhibitors of glucose transport in T. brucei. A graded response of glucose transporter inhibition by these compounds was observed, with Cibacron blue 3GA, CiB, being the most potent. This inhibited transport by up to 90% in a concentration-dependent manner, with an IC 50 of about 19.4 lM. A Dixon plot of dierent concentrations of this triazine and the rate of transport suggested that inhibition may be simple and competitive. The inhibition constant K i was 14.8 lM. Although cytochalasin B has been widely reported to inhibit glucose uptake by mammalian and other eukaryotic glucose transporters, it had no eect at all on the trypanosome transporter at concentrations equivalent to those of the triazines. This may suggest structural dierences between the trypanosome and mammalian glucose transporters and also suggests that the triazine moiety may serve as a template for the design of potent trypanocides targeted at the glucose transporter.

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Triazine Dyes Inhibit HIV‐1 Entry by Binding to Envelope Glycoproteins

Abstract: Abstract:We have attempted to purify envelope (

Cited by 14 publications

References 28 publications

Limited cross-reactivity between different HIV-1 clades

Limited cross-reactivity between different HIV-1 clades

Enhanced detection of respiratory pathogens with nanotrap particles

Triazinyl derivatives that are potent inhibitors of glucose transport in Trypanosoma brucei

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