Tributyrin‐supplemented high‐soya bean meal diets of juvenile black sea bream, Acanthopagrus schlegelii : Study on growth performance and intestinal morphology and structure

Abstract

An 8‐week growth trial was conducted to evaluate the effects of different levels of tributyrin supplementation in a high‐soya bean meal diet on juvenile black sea bream (11.30 ± 0.16 g). The positive control (PC) diet contained 45% fishmeal and 20% soya bean meal, while the negative control (NC) contained 12% fishmeal and 45% soya bean meal. Graded levels of tributyrin were added to the NC diet at 0.05% (TB 0.05), 0.1% (TB 0.1), 0.2% (TB 0.2), 0.4% (TB 0.4) and 0.8% (TB 0.8). Ultimately, the fish fed the PC diet had a higher weight gain and specific growth rate than the fish fed other diets. The fish fed the NC diet had the lowest growth, and TB 0.05–TB 0.2 diets increased growth performance while TB 0.4–TB 0.8 diets caused reduction in growth. Dietary tributyrin supplementation improved protease activity and enhanced antioxidant capacity. Compared with the fish fed the NC diet, the fish fed the tributyrin‐supplemented diets had improved gut morphology and structure, and the results were similar to those of the fish fed the PC diet. Furthermore, the analysis of the dose response with second‐order polynomial regression indicated that the optimum tributyrin supplementation for juvenile black sea bream is 2.24 g/kg in the 45% soya bean meal diet.

Dietary tributyrin modifies intestinal function by altering morphology, gene expression and microbiota profile in common carp ( Cyprinus carpio ) fed all‐plant diets

“…Among the plant-based dietary groups, the activities of digestive enzymes were ameliorated by the increasing TB supplementation level. These results are in line with the recent findings of Volatiana, Wang, et al (2020), who reported that the intestinal protease, lipase and amylase activities were significantly increased in the black sea bream fed the TB-supplemented diets. In the study with snakehead, the greatest protease and lipase activities were observed in the TB-supplemented groups compared with these in chromium yeast groups and bile acid groups (Hou et al, 2019).…”

“…Dietary TB also has been reported to positively affect the growth performance of snakehead (Hou et al, 2019). Recent studies showed that an appropriate dietary TB level improved the growth performance of black sea bream (2.0 g kg −1 -4.0 g kg −1 ) and yellow drum (1.0 g kg −1 -2.0 g kg −1 ) fed high-soya bean meal diets (Tan et al, 2020;Volatiana, Wang, et al, 2020). Similarly, other butyrate products with proper levels (Gao et al, 2011) and giant grouper (Epinephelus lanceolatus) (Lin & Cheng, 2017).…”

Dietary berberine regulates lipid metabolism in muscle and liver of black sea bream (Acanthopagrus schlegelii) fed normal or high-lipid diets

“…The liver samples were stained by Oil Red O following the processes introduced by Volatiana et al (33) . The slide microphotography was observed using an Olympus CX21 microscope.…”

Effect of tributyrin supplementation in high‐soya bean meal diet on growth performance, body composition, intestine morphology and microbiota of juvenile yellow drum (Nibea albiflora)

“…As an esterification product of butyric acid and glycerol, tributyrin was slowly hydrolysed into butyric acids in the entire intestine by pancreatic lipase (Conley et al, ). However, investigation on the effects and mechanisms of tributyrin supplementation in high‐SBM diet on growth performance and intestine health is rare in aquatic animals (Volatiana et al, ).…”