Trichinella britovi recombinant proteins produced in Pichia pastoris expression system for specific IgG antibody detection in the sera of mice and pigs infected with Trichinella spp.

Grzelak, Sylwia; Bień-Kalinowska, Justyna; Stachyra, Anna

doi:10.1016/j.exppara.2022.108386

Cited by 2 publications

(1 citation statement)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They are considered powerful immunoadjuvants that can lead to more substantial antitumor impacts (Banstola et al, 2020). Heat-inducible proteins, including sHSP, HSP60, HSP70, and histone H3, have been isolated from ES products and somatic extracts of T. spiralis, (Sun et al, 2018;Grzelak et al, 2020;Grzelak et al, 2022). Thw HSPs prevent cell death in T. spiralis and sustain homeostasis (Bolhassani and Agi, 2019).…”

Section: Heat Shock Proteinsmentioning

confidence: 99%

Trichinella spiralis as a Potential Antitumor Agent: An Update

Sadr¹,

Yousefsani²,

Simab³

et al. 2023

WVJ

View full text Add to dashboard Cite

Due to the limited success of therapeutic strategies in treating tumors, a new practical potent approach is needed. This review aimed to investigate previous literature related to tumors and Trichinella spiralis (T. spiralis). In recent years, there has been growing interest in utilizing biological, viral, bacterial, yeast, and parasitic agents to cure cancers. According to several studies, some parasites could interferee with the tumors’ growth. There has been much discussion about some parasites’ applications to cure tumors in animals and humans. In studies, T. spiralis was found to have antitumor properties. The active proteins in T. spiralis, such as Caveolin-1, Heat shock proteins, and Ribosomal proteins, are thought to inhibit the growth of cancers, such as melanoma, myeloma, sarcoma, leukemia, stomach cancer, colon cancer, breast cancer, and lung cancer. In addition, these proteins are thought to induce apoptosis in specific neoplastic cells. Accordingly, antigens derived from parasites may be helpful in cancer immunotherapy. However, there are still many unanswered questions regarding Trichinella spiralis’ potential use as a biotherapy agent against cancer. Future studies should focus on the purification of parasite antigens and their use for wider-scale trials in animal models.

show abstract

Section: Heat Shock Proteinsmentioning

confidence: 99%

Trichinella spiralis as a Potential Antitumor Agent: An Update

Sadr¹,

Yousefsani²,

Simab³

et al. 2023

WVJ

View full text Add to dashboard Cite

show abstract

Application of a recombinant novel trypsin from Trichinella spiralis for serodiagnosis of trichinellosis

Han,

Lu,

et al. 2024

Parasites Vectors

View full text Add to dashboard Cite

Background The excretory/secretory (ES) antigen of Trichinella spiralis muscle larvae (ML) is currently the most widely used diagnostic antigen to detect T. spiralis infection. However, this antigen has certain drawbacks, such as a complicated ES antigen preparation process and lower sensitivity during the early phase of infection. The aim of this study was to investigate the features of a novel T. spiralis trypsin (TsTryp) and evaluate its potential diagnostic value for trichinellosis. Methods The TsTryp gene was cloned and recombinant TsTryp (rTsTryp) expressed. Western blotting and an enzyme-linked immunosorbent assay (ELISA) were performed to confirm the antigenicity of rTsTryp. The expression pattern and distribution signature of TsTryp at various life-cycle stages of T. spiralis were analyzed by quantitative PCR, western blotting and the immunofluorescence test. An ELISA with rTsTryp and ML ES antigens was used to detect immunoglobulins G and M (IgG, IgM) in serum samples of infected mice, swine and humans. The seropositive results were further confirmed by western blot with rTsTryp and ML ES antigens. Results TsTryp expression was observed in diverse T. spiralis life-cycle phases, with particularly high expression in the early developmental phase (intestinal infectious larvae and adults), with distribution observed mainly at the nematode outer cuticle and stichosome. rTsTryp was identified by T. spiralis-infected mouse sera and anti-rTsTryp sera. Natural TsTryp protease was detected in somatic soluble and ES antigens of the nematode. In mice infected with 200 T. spiralis ML, serum-specific IgG was first detected by rTsTryp-ELISA at 8 days post-infection (dpi), reaching 100% positivity at 12 dpi, and first detected by ES-ELISA at 10 dpi, reaching 100% positivity at 14 dpi. Specific IgG was detected by rTsTryp 2 days earlier than by ES antigens. When specific IgG was determined in serum samples from trichinellosis patients, the sensitivity of rTsTryp-ELISA and ES antigens-ELISA was 98.1% (51/52 samples) and 94.2% (49/52 samples), respectively (P = 0.308), but the specificity of rTsTryp was significantly higher than that of ES antigens (98.7% vs. 95.4%; P = 0.030). Additionally, rTsTryp conferred a lower cross-reaction, with only three serum samples in total testing positive from 11 clonorchiasis, 20 cysticercosis and 24 echinococcosis patients (1 sample from each patient group). Conclusions TsTryp was shown to be an early and highly expressed antigen at intestinal T. spiralis stages, indicating that rTsTryp represents a valuable diagnostic antigen for the serodiagnosis of early Trichinella infection. Graphical Abstract

show abstract

Trichinella britovi recombinant proteins produced in Pichia pastoris expression system for specific IgG antibody detection in the sera of mice and pigs infected with Trichinella spp.

Cited by 2 publications

References 46 publications

Trichinella spiralis as a Potential Antitumor Agent: An Update

Trichinella spiralis as a Potential Antitumor Agent: An Update

Application of a recombinant novel trypsin from Trichinella spiralis for serodiagnosis of trichinellosis

Contact Info

Product

Resources

About