Trichinella: Differing effects of antigens from encapsulated and non-encapsulated species on in vitro nitric oxide production

Andrade, M. Amparo; Siles-Lucas, Mar; López‐Abán, Julio; Nogal-Ruiz, Juan José; Pérez-Arellano, José Luís; Martínez-Fernández, A. R.; Muro, Antonio

doi:10.1016/j.vetpar.2006.07.026

Cited by 18 publications

(14 citation statements)

References 13 publications

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“…Andrade et al (2007) described interspecies results from in vitro study, where the differences in NO production of macrophages after stimulation with L1 antigens from encapsulated and non-encapsulated Trichinella species were demonstrated. Immunochemical variety of larval T. spiralis and T. pseudospiralis antigens have been confirmed in several studies (Turčeková et al 1997;Ros-Moreno et al 2002;Robinson et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Development of cellular immune response of mice to infection with low doses of Trichinella spiralis, Trichinella britovi and Trichinella pseudospiralis larvae

Hurníková

Kołodziej‐Sobocińska

2010

Parasitol Res

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The murine cellular immune response to the infection with ten larvae of encapsulating (Trichinella spiralis, Trichinella britovi) and non-encapsulating species (Trichinella pseudospiralis) was studied. Both T. spiralis and T. britovi stimulated the proliferation of splenic T and B lymphocytes during the intestinal phase of infection, but T. spiralis activated the proliferative response also at the muscle phase, particularly in B cells. Non-encapsulating T. pseudospiralis stimulated the proliferation of T and B cells only on day 10 post-infection (p.i.) and later at the muscle phase. The numbers of splenic CD4 and CD8 T cells of T. spiralis infected mice were significantly increased till day 10 p.i., i.e., at the intestinal phase, and then at the late muscle phase, on day 60 p.i. T. britovi infection increased the CD4 and CD8 T cell numbers only on day 30 p.i. Decreased numbers of CD4 and CD8 T cells after T. pseudospiralis infection suggest a suppression of cellular immunity. Both encapsulating Trichinella species induced the Th2 response (cytokines interleukin-5 (IL-5) and interleukin-10) at the intestinal phase and the Th2 dominant response at the advanced muscle phase. Interferon-γ (IFN-γ) production (Th1 type) started to increase with migrating newborn larvae from day 15 p.i. till the end of the experiment. IL-5 production was suppressed during the intestinal phase of T. pseudospiralis infection. The immune response to T. pseudospiralis was directed more to the Th1 response at the muscle phase, the high IFN-γ production was found on day 10 p.i. and it peaked on days 45 and 60 p.i.

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Section: Introductionmentioning

confidence: 99%

Development of cellular immune response of mice to infection with low doses of Trichinella spiralis, Trichinella britovi and Trichinella pseudospiralis larvae

Hurníková

Kołodziej‐Sobocińska

2010

Parasitol Res

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“…after a high infective dose. Andrade et al (2007) described interspecies results from in vitro study, where the differences in NO production of macrophages after stimulation with L1 antigens from encapsulated and non-encapsulated trichinellas were demonstrated. Immunochemical variety of larval T. spiralis and T. pseudospiralis antigens had been confirmed by Turčeková et al (1997).…”

Section: Introductionmentioning

confidence: 99%

Kinetics of specific humoral immune response of mice infected with low doses of Trichinella spiralis, T. britovi, and T. pseudospiralis larvae

Hurníková¹,

Kołodziej‐Sobocińska

2010

Helminthologia

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The specific humoral immune response of the host (mouse) to the infection with low doses of larvae of encapsulating (Trichinella spiralis, T. britovi) and non-encapsulating (T. pseudospiralis) species was studied. Mice were experimentally infected with 10 muscle larvae of the parasite to simulate natural conditions of the infection in rodents, important reservoirs of trichinellosis. The low infective dose of T. spiralis and T. britovi did not evoke an increased specific IgM response, which is typical for the acute infection. Only T. pseudospiralis induced a higher specific IgM level in the intestinal phase of the infection, till day 30 p.i. The low infective dose of T. spiralis larvae stimulated a specific IgG1 production from day 20 p.i. with a strong increase on day 45 p.i., but T. britovi infection on day 60 p.i. Specific IgG1 antibodies were not detected in T. pseudospiralis infection. The production of IgG2a and IgG2b antibodies was again earlier and more expressive in T. spiralis infection from day 45 p.i., in contrast to T. britovi, where these antibodies were increased on day 60 p.i. Only IgG2b isotype was detected in T. pseudospiralis infection on days 45 and 60 p.i., however in very low values in comparison with encapsulating species. Results conclude the low infective dose of T. spiralis, T. britovi, and T. pseudospiralis induced a late seroconversion in infected mice. T. spiralis caused earlier and more intensive specific antibody response, from day 45 p.i, when antigens from newborn and muscle larvae were accumulated, on the contrary to T. britovi and T. pseudospiralis, which induced specific antibody response from day 60 p.i.

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“…We investigated the stimulatory/inhibitory role of L1 antigens from four encapsulated ( Trichinella spiralis , Trichinella britovi , Trichinella nelson- and Trichinella nativa ) and one nonencapsulated (Trichinella pseudospiralis ) species of Trichinella on NO production. Our results demonstrated that encapsulated and non-encapsulated Trichinella species differ in their ability to stimulate the NO secretion from the host macrophages [29]. These differences could be related to the complete and incomplete formation of the nurse cells in trichinellosis.…”

Section: Do Helminths Induce No Production By the Host Cells And mentioning

confidence: 85%

Nitric Oxide and Respiratory Helminthic Diseases

Muro

Pérez-Arellano

2010

Journal of Biomedicine and Biotechnology

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Nitric oxide (NO) is a very simple molecule that displays very important functions both in helminths (mainly those involved in respiratory pathology) and in mammalian hosts. In this paper we review four issues related to interaction of NO and lung helminthic diseases. Firstly, we evaluated data available on the NO synthesis and release by helminths and their biological role. Next, we summarized the effect of antigens obtained from different phases of the biological cycle on NO production by host mammalian cells (mainly from human sources). Thirdly, we revised the evaluation of NO on the biological activities and/or the viability of respiratory helminths. Lastly, the deleterious consequences of increased production of NO during helminthic human infection are detailed.

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Trichinella: Differing effects of antigens from encapsulated and non-encapsulated species on in vitro nitric oxide production

Cited by 18 publications

References 13 publications

Development of cellular immune response of mice to infection with low doses of Trichinella spiralis, Trichinella britovi and Trichinella pseudospiralis larvae

Development of cellular immune response of mice to infection with low doses of Trichinella spiralis, Trichinella britovi and Trichinella pseudospiralis larvae

Kinetics of specific humoral immune response of mice infected with low doses of Trichinella spiralis, T. britovi, and T. pseudospiralis larvae

Nitric Oxide and Respiratory Helminthic Diseases

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