Trichinella spiralis shares epitopes with human autoantigens

Radović, Ivana; Gruden-Movsesijan, Alisa; Ilić, Nataša; Mostarica-Stojković, Marija; Sofronić-Milosavljević, Ljiljana

doi:10.1590/s0074-02762012000400010

Cited by 16 publications

(10 citation statements)

References 27 publications

(26 reference statements)

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“…All human sera that were considered positive for T. spiralis infection by IFA also bound to the 45, 49, 53 kDa bands in Wb. However, previous investigations revealed that a number of sera from pregnant women or malignancies [19], as well as from patients with autoantibodies [20,21] and other parasitoses [22][23][24][25][26] gave false positive results in ELISA. When examined in Wb, sera from patients with Trichinella-irrelevant infections or other diseases showed presence of antibodies reactive with T. spiralis ES components of different Mw, some of which belong to the Trichinella-characteristic triad.…”

Section: Discussionmentioning

confidence: 99%

“…However, this cross-reactivity never resulted in the appearance of a Trichinella-specifi c band triad at 45, 49 and 53 kDa. Cross-reactivity occurs because of the existence of evolutionary highly conserved epitopes which are common for different pathogens and can also be recognized by some autoantibodies [21]. Sera from healthy blood donors, that tested negative in ELISA for the detection of anti-Trichinella antibodies, were used as negative controls.…”

Section: Discussionmentioning

confidence: 99%

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Characteristic band pattern in western blots for specific detection of anti-Trichinella spiralis antibodies in different host species

Ilić¹,

Gruden-Movsesijan²,

Živojinovic³

et al. 2014

Acta Veterinaria

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Western blot (Wb) is considered to be the gold standard test for Trichinella infection serology, since this method allows specific Trichinella antigens to be distinguished from cross-reactive antigens. This is not the case with widely used antibody assay techniques - indirect immunofluorescence and ELISA - which are sensitive, but subject to crossreactions that make the interpretation of weakly positive results difficult. Application of Trichinella spiralis muscle larvae excretory-secretory (ES) antigens for the specific antibody detection in ELISA resulted in improved specificity compared to that of crude worm extract that was previously in use, but since production of ES has not yet been standardized, differences among laboratories occur. For this reason, the Wb profile of serum samples from different T. spiralis infected host species: human, horse, swine and dog, was investigated in the Serbian National Reference Laboratory for Trichinellosis (NRLT). The common feature of the obtained Wb profiles was the appearance of a triad of bands with molecular masses (Mw) of 45, 49, and 53 kDa. The very same triad was recognized by a monoclonal antibody (mAb) 7C2C5 specific for an immunodominant epitope unique to the muscle larvae stage of all species in the genus Trichinella. Inhibition studies confirmed that mAb and anti-Trichinella antibodies from sera competed for the same parasite epitope. Based on the obtained results, the NRLT introduced the recognition of the above mention triad as the basis for specific anti-Trichinella antibodies detection in the sera of infected hosts.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Characteristic band pattern in western blots for specific detection of anti-Trichinella spiralis antibodies in different host species

Ilić¹,

Gruden-Movsesijan²,

Živojinovic³

et al. 2014

Acta Veterinaria

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“…This finding could be explained by the IL-10 being produced by crossreactive regulatory T or B cells that would already had been stimulated by autoantigens in vivo . Crossreactivities between helminth antigens and autoantigens have indeed been described (31–35). …”

Section: Do Autoreactive and Allergen-reactive Immune Regulatory Cellmentioning

confidence: 99%

“…Several examples of crossreactivity of pathogens with autoantigens have been reported [e.g., (31–35, 39–45)], some of which due to phylogenetic homologies (32–34, 42, 44). A particular study has shown that, depending on the HLA allele, 80–290 MHC II-binding peptides from Mycobacterium tuberculosis crossreact with human autoantigens (45).…”

Section: The Parasites’ Good Luck: Some Crossreactivity May Protect Tmentioning

confidence: 99%

Antigen mimicry between infectious agents and self or environmental antigens may lead to long-term regulation of inflammation

et al. 2013

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“…Antibodies present in the sera of T. spiralis -infected human and animals always recognize this glycoprotein, pointing to a role in the induction of the immune response [52, 53]. The fact that 53 kDa glycoprotein could be frequently recognized by antibodies in the sera from patients with autoimmune diseases [62] adds to its role in immunomodulation. Data obtained by testing immunogenicity of 53 kDa glycoprotein using monoclonal antibodies indicate that this protein bears epitopes that are specific for T. spiralis as well as an epitope shared by all encapsulated Trichinella [36, 63].…”

Section: Es L1 Antigens Of T Spiralis Muscle Larvaementioning

confidence: 99%

Secretory Products ofTrichinella spiralisMuscle Larvae and Immunomodulation: Implication for Autoimmune Diseases, Allergies, and Malignancies

Sofronić-Milosavljević

Ilić

Pinelli

et al. 2015

Journal of Immunology Research

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Trichinella spiralis has the unique ability to make itself “at home” by creating and hiding in a new type of cell in the host body that is the nurse cell. From this immunologically privileged place, the parasite orchestrates a long-lasting molecular cross talk with the host through muscle larvae excretory-secretory products (ES L1). Those products can successfully modulate parasite-specific immune responses as well as responses to unrelated antigens (either self or nonself in origin), providing an anti-inflammatory milieu and maintaining homeostasis. It is clear, based on the findings from animal model studies, that T. spiralis and its products induce an immunomodulatory network (which encompasses Th2- and Treg-type responses) that may allow the host to deal with various hyperimmune-associated disorders as well as tumor growth, although the latter still remains unclear. This review focuses on studies of the molecules released by T. spiralis, their interaction with pattern recognition receptors on antigen presenting cells, and subsequently provoked responses. This paper also addresses the immunomodulatory properties of ES L1 molecules and how the induced immunomodulation influences the course of different experimental inflammatory and malignant diseases.

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Trichinella spiralis shares epitopes with human autoantigens

Cited by 16 publications

References 27 publications

Characteristic band pattern in western blots for specific detection of anti-Trichinella spiralis antibodies in different host species

Characteristic band pattern in western blots for specific detection of anti-Trichinella spiralis antibodies in different host species

Antigen mimicry between infectious agents and self or environmental antigens may lead to long-term regulation of inflammation

Secretory Products ofTrichinella spiralisMuscle Larvae and Immunomodulation: Implication for Autoimmune Diseases, Allergies, and Malignancies

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