Trichoderma asperellum (NST-009): A potential native antagonistic fungus to control Cercospora leaf spot and promote the growth of 'Green Oak' lettuce (Lactuca sativa L.) cultivated in the commercial NFT hydroponic system

Promwee, Athakorn; Intana, Warin

doi:10.17221/69/2021-pps

Cited by 13 publications

(7 citation statements)

References 32 publications

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“…Numerous studies also have demonstrated the antagonistic effect of T. asperellum against several fungal pathogens. Examples include a hydroponics experiment with 'Green Oak' lettuce (Lactuca sativa L.) in Thailand, where T. asperellum NST-009 reduced leaf spot caused by Cercospora by 67.51% compared to the control (Promwee and Intana, 2022). Also, the mycelial growth of A. porri (causative agent of purple spot-on onion) was inhibited in 56% by T. asperellum in dual culture (Camacho-Luna et al, 2021).…”

Section: Discussion Discussion Discussionmentioning

confidence: 99%

“…López-Valenzuela et al (2022) found that two isolates of T. asperellum (TB and TM) produced IAA at 25.5 μg•mL -1 and 24.5 μg•mL -1 , and AG3 (2.51 ± 0.127 μg•mL -1 ; 2.46 ± 0.104 μg•mL -1 ). Promwee and Intana (2022) reported that T. asperellum significantly stimulated the growth of Lactuca sativa L. by increasing plant height (8.62%), number of leaves (18.39%), root fresh weight (39.26%) and shoot fresh weight (25.71%). Further, Wang et al (2021) reported that T. asperellum promoted plant growth in an apple orchard in Shandong Province, China.…”

Section: Introduction Introduction Introduction Introductionmentioning

confidence: 99%

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Application of Trichoderma asperellum in apple trees as a growth regulator and antagonist for the control of Alternaria sp.

MADRID-MOLINA

Pérez-Álvarez

Escobedo-Bonilla

et al. 2023

Not Bot Horti Agrobo

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Chihuahua state is the main apple producer in Mexico. The present study aimed to evaluate the effectiveness of Trichoderma asperellum as a plant growth regulator and antagonist against Alternaria sp. Three treatments were used: T1 = control, T2 = 200 mL of T. asperellum per tree; T3 = 100 mL of T. asperellum per tree. Agronomic variables were evaluated including number of leaves, shoots and flowers, disease incidence, and trunk thickness. Alternaria sp. was isolated from apple leaves at the experimental site in Guerrero County, Chihuahua, Mexico, and it was grown on solid PDA medium for morphological characterization. The molecular characterization was done by PCR using primers ITS1 and ITS4 producing products of 700 bp which were sequenced, submitted to GenBank (acc. no. OQ344593) and used for further phylogenetic analysis through Bayesian inference approach. Three clades were identified and the polytome topography recovered from clade 2 indicates a high genetic similarity with A. tenuissima (100% similarity according to BLAST). The analysis of T. asperellum as growth regulator only showed significant differences in trunk thickness and displayed higher values with T3 (p≤0.05). The presence of A. tenuissima was only observed in the control, which indicated the ability of Trichoderma to control the fungus. In this study T. asperellum was not an efficient plant growth regulator, but it was a good antagonist, and hence it can be recommended to control A. tenuissima. This is the first record of A. tenuissima in apple trees in Mexico. These results indicate that T. asperellum showed no benefit as plant growth regulator when applied to apple trees of the ‘Granny Smith’ variety.

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Section: Discussion Discussion Discussionmentioning

confidence: 99%

Section: Introduction Introduction Introduction Introductionmentioning

confidence: 99%

Application of Trichoderma asperellum in apple trees as a growth regulator and antagonist for the control of Alternaria sp.

MADRID-MOLINA

Pérez-Álvarez

Escobedo-Bonilla

et al. 2023

Not Bot Horti Agrobo

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“…Mycoparasitism has also been determined to be an effective mechanism for inhibiting the pathogenic mycelial growth of Cercospora hypha, which causes foliar disease. All strains of T. asperellum tested in lettuce showed mycoparasitic ability by colonizing C. hypha, drilling holes in its mycelia, and reproducing conidia [60]. Additionally, in cucumbers attacked by Fusarium, the avirulent strain of the same genus (F. oxysporum CS-20) was determined to generate strong antagonistic activity, produce enzymes such as chitinase, and induce ISR, resulting in reduced DSI (from 61.67 to 41.67%) [61].…”

Section: Pgpm As Biocontrol Agentsmentioning

confidence: 99%

Introducing the Power of Plant Growth Promoting Microorganisms in Soilless Systems: A Promising Alternative for Sustainable Agriculture

Mourouzidou

Ντίνας²,

Tsaballa³

et al. 2023

Sustainability

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Soilless systems, such as hydroponics and aquaponics, are gaining popularity as a sustainable alternative to traditional soil-based agriculture, aiming at maximizing plant productivity while minimizing resource use. Nonetheless, the absence of a soil matrix poses challenges that require precise management of nutrients, effective control of salinity stress, and proactive strategies to master disease management. Plant growth-promoting microorganisms (PGPM) have emerged as a promising solution to overcome these issues. Research demonstrated that Bacillus, Pseudomonas, and Azospirillum are the most extensively studied genera for their effectiveness as growth promoters, inducing changes in root architecture morphology. Furthermore, PGPM inoculation, either alone or in synergy, can reverse the effects of nutrient deficiency and salt stress. The genera Pseudomonas and Trichoderma were recognized for their solid antagonistic traits, which make them highly effective biocontrol agents in hydroponic systems. The latest findings indicate their ability to significantly reduce disease severity index (DSI) through mycoparasitism, antibiosis, and induced systemic resistance. In aquaponic systems, the inoculation with Bacillus subtilis and Azospirillum brasilense demonstrated increased dissolved oxygen, improving water quality parameters and benefiting plant and fish growth and metabolism. This review also establishes the interaction variability between PGPM and growing media, implying the specificity for determining inoculation strategies to maximize the productivity of soilless cultivation systems. These findings suggest that using PGPM in soil-free settings could significantly contribute to sustainable crop production, addressing the challenges of nutrient management, disease control, and salinity issues.

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“…The pathogen can spread easily through rain splashes and results in the dispersal of the conidia. The control and management of Cercospora disease are practiced through the use of harmful chemical fungicides due to the application of these synthetic fungicides signi cant chemical contamination can accumulate in agricultural soil, and shows a negative impact on soil microbes, and continuous application of fungicides creates resistance in pathogen (Athakorn et al 2022). An eco-friendly protocol for the control and management of Cercospora disease and increasing resistance in mung bean plants becomes an alternative approach in sustainable agriculture.…”

Section: Introductionmentioning

confidence: 99%

Formulation-based antagonistic endophyte Amycolatopsis sp. SND-1 triggers defense response in Vigna radiata (L.) R. Wilczek. (Mung bean) against Cercospora leaf spot disease

2023

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In the present work, Amycolatopsis sp. SND-1 (SND-1) was isolated from Cleome chellidonii Linn. (C. chellidonii) was performed as biocontrol and resistance elicitor in Vigna radiata (L.) R. Wilczek (mung bean) plants against Cercospora leaf spot causing pathogen Cercospora canescens (C. canescens). The SND-1 isolate showed 74% of inhibition against C. canescens in dual culture and in-vitro plant growth trials exhibited production of indole acetic acid, gibberellic acid, cytokinin, ammonia, hydrogen cyanide, and siderophore and phosphate solubilization. In-vivo study with talcum formulation of SND-1 revealed a signi cant increase in plant root length, shoots length, root and shoot fresh weight, and reduced the disease severity in treated mung bean plants. Triggering of resistance by SND-1 formulation was studied by histochemical depositions and biochemical defense enzymes resulted that the acceleration in defense response in comparison with control plants. The bioactive endophytic Amycolatopsis sp. SND-1 enhanced the defense against C. canescens infection; hence it can be used as a biological control agent in mung bean cultivars.

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Trichoderma asperellum (NST-009): A potential native antagonistic fungus to control Cercospora leaf spot and promote the growth of 'Green Oak' lettuce (Lactuca sativa L.) cultivated in the commercial NFT hydroponic system

Cited by 13 publications

References 32 publications

Application of Trichoderma asperellum in apple trees as a growth regulator and antagonist for the control of Alternaria sp.

Application of Trichoderma asperellum in apple trees as a growth regulator and antagonist for the control of Alternaria sp.

Introducing the Power of Plant Growth Promoting Microorganisms in Soilless Systems: A Promising Alternative for Sustainable Agriculture

Formulation-based antagonistic endophyte Amycolatopsis sp. SND-1 triggers defense response in Vigna radiata (L.) R. Wilczek. (Mung bean) against Cercospora leaf spot disease

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