Trichoderma improves the growth of Leymus chinensis

Zhang, Fengge; Huo, Yunqian; Xu, Xixi; Hu, Jian; Sun, Xiao; Xiao, Yan; Zhang, Yingjun

doi:10.1007/s00374-018-1292-7

Cited by 32 publications

(21 citation statements)

References 63 publications

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“…Leymus chinensis is an ecologically and economically important herbage that is widely distributed in the grasslands of Inner Mongolia and is a native perennial rhizome grass with prominent forage value and great palatability (Zhang et al , 2018). However, knowledge regarding the root microorganisms associated with L. chinensis remains limited, although root‐associated microbiomes have been extensively studied in other related plants (Yamamoto et al , 2018; Poudel et al , 2019; Singer et al , 2019).…”

Section: Discussionmentioning

confidence: 99%

Microbial assemblages associated with the rhizosphere and endosphere of an herbage, Leymus chinensis

Chen

Chao

et al. 2020

Microbial Biotechnology

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Root-associated microbiomes play significant roles in plant productivity, health and ecological services. However, our current understanding of the microbial assemblages in the rhizosphere and endosphere of herbage is still limited. To gain insights into these microbial assemblages, Illumina MiSeq high-throughput sequencing was performed to investigate the characteristics of microbial communities of an herbage, Leymus chinensis. Hierarchical clustering analysis and principal coordinate analysis (PCoA) results showed that microbial communities of the rhizosphere and endosphere samples were clearly distinguished. Rhizosphere soil communities showed a greater sensitivity than root endosphere communities using linear discriminant analysis (LDA) effect size (LEfSe). Rhizosphere and endosphere communities performed their respective functions in the soil as a cohesive collective, and Rhizobiales were observed to function as generalists. Redundancy analysis (RDA) and variance partitioning analysis (VPA) results revealed that the contribution of the interaction between soil physicochemical parameters and soil enzymes was greater than their individual contributions. In summary, this study is the first to elucidate the microbial diversity and community structure of L. chinensis and compare the diversity and composition between rhizospheric and endosphere microbiomes.

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Section: Discussionmentioning

confidence: 99%

Microbial assemblages associated with the rhizosphere and endosphere of an herbage, Leymus chinensis

Chen

Chao

et al. 2020

Microbial Biotechnology

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“…The fungus responsible for A. carmichaelii root rot, namely F. oxysporum , also decreased markedly in its density in the rhizosphere of CF3‐treated plants. Recently, Bacillus amyloliquefaciens and Trichoderma rossicum were shown to control soil‐borne diseases by increasing the abundance of possibly beneficial microbes while decreasing the abundance of pathogenic microbes (Xiong et al ; Zhang et al ). Similarly, through our culturing test, we found that field application of the P. griseofulvum CF3 agent increased the density of some microbes possibly beneficial to plant growth while decreasing other microbes known to cause plant diseases.…”

Section: Discussionmentioning

confidence: 99%

Biocontrol effects of Penicillium griseofulvum against monkshood ( Aconitum carmichaelii Debx.) root diseases caused by Sclerotium rolfsiii and Fusarium spp.

Qiao

Wei

et al. 2019

J Appl Microbiol

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Aims: The aims of this study were to investigate the biocontrol effects of Penicillium griseofulvum strain CF3 and its mechanisms against soil-borne root pathogens (Fusarium oxysporum and Sclerotium rolfsii) of the medical plant Aconitum carmichaelii Debx. Methods and Results: The effects of P. griseofulvum strain CF3 were evaluated with regard to the hyphal growth of S. rolfsii and F. oxysporum, the sclerotial formation and germination of S. rolfsii and its expression of sclerotiaformation-related genes. A field experiment was conducted to explore how strain CF3 controls the severity of soil-borne diseases, promotes the growth of A. carmichaelii plants and mediates shifts in the culturable rhizosphere microbial populations. The results showed that treatment with a cell-free culture filtrate of strain CF3 considerably inhibited the hyphal growth of both S. rolfsii and F. oxysporum, in addition to limiting the sclerotial formation and germination of S. rolfsii. Three genes related to sclerotial formation (ArsclR, ArnsdD1 and ArnsdD2) were predicted in S. rolfsii and their expression was found suppressed by the CF3 treatment. Field application of the CF3 biocontrol agent in a powder form (1Á9 9 10 10 conidia per gram of substrate) reduced soil-borne disease severity by 15Á0%. The shoot and root growth of A. carmichaelii plants was promoted by 61Á6 and 83Á1% respectively, as the biocontrol strain massively colonized the rhizosphere soil. The CF3 treatment also markedly reduced the density of some known species harmful to plants while increasing the density of some beneficial species in the rhizosphere soil. Significance and Impact of the Study: Genes related to sclerotia formation of S. rolfsii are predicted for the first time and their expression patterns in the presence of P. griseofulvum strain CF3 are evaluated. This comprehensive study provides a candidate fungal biocontrol strain and reveals its potential mechanisms against S. rolfsii and F. oxysporum in A. carmichaelii plants.

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“…The primer pair (ITS1 S: 5′-TACAACTCCCAAACCCAATGT GA-3; ITS1 R: 5′-CCGTTGTTGAAAGTTTTGATTCATTT-3′) (Lopez-Mondejar et al, 2010) and probe 5′-FAM-AACTCTTTTTGTATACCCCCTCGCGGGT-TMR-3′ (FAM: 6-carboxyfluorescein, TAMRA: 6-carboxy-tetramethylrhodamine) (Zhang et al, 2018b) were used to amplify the Trichoderma ITS region. In the real-time PCR amplifications, Premix Ex Taq TM (Takara) had a volume of 20 μl: 10 μl of Premix Ex Taq TM (Takara Bio Inc., Japan), 0.4 μl of Rox Reference Dye II (50 ×), 0.4 μl of each primer (10 μM), 0.8 μl of TaqMan probe (10 pM), 2 μl of template DNA and 6 μl of sterilized double-distilled water.…”

Section: Methodsmentioning

confidence: 99%

“…Trichoderma species are important plant growth-promoting fungi (PGPF) in soils that have been reported to significantly facilitate plant growth and development (Singh et al, 2015) through numerous mechanisms: increasing solubilization of soil nutrients (Yadav et al, 2009; Kapri and Tewari, 2010), increasing nutrient efficacy and recycling (Azarmi et al, 2011), releasing plant growth stimulatory agents (Contreras-Cornejo et al, 2009) and inducing systemic resistance (Shoresh et al, 2010). Several studies show that Trichoderma -inoculation has significant promotion effects on the growth of many plant species (Howell et al, 2000; Zhang et al, 2013a), including those of pasture fields (Lee et al, 2015; Zhang et al, 2018b). Yuan et al (2018) has demonstrated that five Trichoderma strains (3 strains of Trichoderma harzianum , 1 strain of Trichoderma longibrachiatum and Trichoderma reesei ) substantially facilitate the growth and improve the nutritional quality of orchard grass.…”

Section: Introductionmentioning

confidence: 99%

Trichoderma-Inoculation and Mowing Synergistically Altered Soil Available Nutrients, Rhizosphere Chemical Compounds and Soil Microbial Community, Potentially Driving Alfalfa Growth

Zhang

Huo

et al. 2019

Front. Microbiol.

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Trichoderma spp. are proposed as major plant growth-promoting fungi (PGPF) to increase plants growth and productivity. Mowing can stimulate aboveground regrowth to improve plant biomass and nutritional quality. However, the synergistic effects of Trichoderma and mowing on plants growth, particularly the underlying microbial mechanisms mediated by rhizosphere soil chemical compounds, have rarely been reported. In the present study, we employed Trichoderma harzianum T-63 and conducted a pot experiment to investigate the synergistic effect of Trichoderma-inoculation and mowing on alfalfa growth, and the potential soil microbial ecological mechanisms were also explored. Alfalfa treated with Trichoderma-inoculation and/or mowing (T, M, and TM) had significant (P < 0.05) increases in plant shoot and root dry weights and soil available nutrients (N, P, and K), compared with those of the control (CK). Non-metric multidimensional scaling (NMDS) demonstrated that the rhizosphere chemical compounds and soil bacterial and fungal communities were, respectively, separated according to different treatments. There was a clear significant (P < 0.05) positive correlation between alfalfa biomass and the relative abundance of Trichoderma (R2 = 0.3451, P = 0.045). However, Pseudomonas, Flavobacterium, Arthrobacter, Bacillus, Agrobacterium, and Actinoplanes were not significantly correlated with alfalfa biomass. According to structure equation modeling (SEM), Trichoderma abundance and available P served as primary contributors to alfalfa growth promotion. Additionally, Trichoderma-inoculation and mowing altered rhizosphere soil chemical compounds to drive the soil microbial community, indirectly influencing alfalfa growth. Our research provides a basis for promoting alfalfa growth from a soil microbial ecology perspective and may provide a scientific foundation for guiding the farming of alfalfa.

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Trichoderma improves the growth of Leymus chinensis

Cited by 32 publications

References 63 publications

Microbial assemblages associated with the rhizosphere and endosphere of an herbage, Leymus chinensis

Microbial assemblages associated with the rhizosphere and endosphere of an herbage, Leymus chinensis

Biocontrol effects of Penicillium griseofulvum against monkshood ( Aconitum carmichaelii Debx.) root diseases caused by Sclerotium rolfsiii and Fusarium spp.

Trichoderma-Inoculation and Mowing Synergistically Altered Soil Available Nutrients, Rhizosphere Chemical Compounds and Soil Microbial Community, Potentially Driving Alfalfa Growth

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