Triclosan-tolerant bacteria: changes in susceptibility to antibiotics

Cottell, Alison; Denyer, Stephen Paul; Hanlon, G. W.; Ochs, D.; Maillard, Jean‐Yves

doi:10.1016/j.jhin.2009.01.014

Cited by 64 publications

(43 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This is important as it has been shown that the use of biocides can select for a bacterial sub-population which may have different growth characteristics (Maillard 2007) and which may then have a different effect on the host tissues. Bacteria that survive exposure to sub-MIC levels of biocides may develop a resistant to the biocide, and in some cases this can lead to further resistance to other biocides or antibiotics which have a similar mechanism of action (Cottell et al 2009). This may be of particular significance with SAG bacteria due to their role in abscess formation, as antibiotics are commonly used in treatment of abscesses.…”

Section: Discussionmentioning

confidence: 99%

“…Streptococcus species, by disrupting the phospholipid membrane of bacteria and interfere with its normal functions (Cottell et al 2009;Villalain et al 2001). Leakage titration experiments have been used to study the effects of triclosan on the bacterial membrane of the oral bacteria Pseudomonas ginigivalis and Streptococcus sohrinus, which showed that whilst the bacterial growth was inhibited there was negligible cell leakage.…”

Section: At High Concentrations Triclosan Acts Against Most Susceptibmentioning

confidence: 99%

See 1 more Smart Citation

Development of an Ex Vivo Coculture System to Model Pulpal Infection by Streptococcus anginosus Group Bacteria

Roberts¹,

Maillard²,

Waddington³

et al. 2013

Journal of Endodontics

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Section: At High Concentrations Triclosan Acts Against Most Susceptibmentioning

confidence: 99%

Development of an Ex Vivo Coculture System to Model Pulpal Infection by Streptococcus anginosus Group Bacteria

Roberts¹,

Maillard²,

Waddington³

et al. 2013

Journal of Endodontics

View full text Add to dashboard Cite

“…Recently, US Environmental Protection Agency (EPA) has suggested that TCS could cause potential human health effects, so they have started to conduct a review of TCS this year (http:// www.epa.gov/oppsrrd1/REDs/factsheets/triclosan_fs.htm). Additionally, it is also necessary to improve our knowledge on the environmental fate of TCS due to its possible bioaccumulation and toxicity in organisms, as well as the possibility of this compound promoting the predominance of antibiotic-resistant bacteria (Cottell et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Determining the distribution of triclosan and methyl triclosan in estuarine settings

2014

View full text Add to dashboard Cite

h i g h l i g h t sSimultaneous extraction of TCS and MTCS by SBSE-LD and PLE-in-cell-clean up. Analysis of estuarine sediment and water samples and from photodegradation assays. Distribution of analytes in estuaries depends on wastewater sources and tides. TCS can be both photodegraded or converted to MTCS in estuaries. a r t i c l e i n f o a b s t r a c tWe have developed a method for the analysis of two sewage-derived contaminants: triclosan (TCS), an antibacterial agent, and methyl triclosan (MTCS), a TCS metabolite. For solid samples (4 g), extraction and cleanup were integrated into the same step using pressurized liquid extraction (PLE) with in-cellclean-up (1 g of florisil). The extraction was performed using dichloromethane at 100°C, 1500 psi and 3 static extraction cycles of 5 min each. For water samples (100 mL), stir bar sorptive extraction-liquid desorption (SBSE-LD) was used. Bars were stirred for 10 h and analytes were later desorbed using acetonitrile. Finally, MTCS and a silylated derivative of TCS were determined by gas chromatography-mass spectrometry (GC-MS). Recovery experiments in water and sediments were performed and the results ranged from 67% to 78%. Limits of detection (LODs) were 5 ng L À1 for TCS and 1 ng L À1 for MTCS, in water samples, and 0.1 ng g À1 for TCS and MTCS in solid samples. The method was applied then to determine the levels of these compounds in the estuary of Guadalete River (SW Spain). TCS and MTCS concentrations up to 9.6 ng g À1 in sediments and 310 ng L À1 in water were measured. Their distribution was strongly influenced by the presence of wastewater sources, treated and untreated, along the sampling area, where maximum concentrations were detected. Highest values were reached in the water column during low tides as the water volume in the estuary becomes lower.

show abstract

“…There is therefore growing concern about their potential risk in soils and the receiving aquatic environment. As examples, bioaccumulation in fish has been measured for some hydrophobic PPCPs (UV filters, fragrances…) [8]; antibiotics can promote resistant bacteria populations [9]; and endocrine-disrupting compounds (EDCs) may cause hermaphroditism [10].…”

Section: Introductionmentioning

confidence: 99%

Environmentally friendly analysis of emerging contaminants by pressurized hot water extraction–stir bar sorptive extraction–derivatization and gas chromatography–mass spectrometry

2012

View full text Add to dashboard Cite

This work describes the development, optimization, and validation of a new method for the simultaneous determination of a wide range of pharmaceuticals (betablockers, lipid regulators…) and personal care products (fragrances, UV filters, phthalates…) in both aqueous and solid environmental matrices. Target compounds were extracted from sediments using pressurized hot water extraction followed by stir bar sorptive extraction. The first stage was performed at 1,500 psi during three static extraction cycles of 5 min each after optimizing the extraction temperature (50-150°C) and addition of organic modifiers (% methanol) to water, the extraction solvent. Next, aqueous extracts and water samples were processed using polydimethylsiloxane bars. Several parameters were optimized for this technique, including extraction and desorption time, ionic strength, presence of organic modifiers, and pH. Finally, analytes were extracted from the bars by ultrasonic irradiation using a reduced amount of solvent (0.2 mL) prior to derivatization and gas chromatography-mass spectrometry analysis. The optimized protocol uses minimal amounts of organic solvents (<10 mL/sample) and time (≈8 h/sample) compared to previous existing methodologies. Low standard deviation (usually below 10 %) and limits of detection (sub-ppb) vouch for the applicability of the methodology for the analysis of target compounds at trace levels. Once developed, the method was applied to determine concentrations of these compounds in several types of sample (wastewater, seawater, pore water, and sediment) from Cadiz Bay (SW Spain). To our knowledge, these findings represent the first information available on the presence of some of the target compounds in the marine environment.

show abstract

Triclosan-tolerant bacteria: changes in susceptibility to antibiotics

Cited by 64 publications

References 12 publications

Development of an Ex Vivo Coculture System to Model Pulpal Infection by Streptococcus anginosus Group Bacteria

Development of an Ex Vivo Coculture System to Model Pulpal Infection by Streptococcus anginosus Group Bacteria

Determining the distribution of triclosan and methyl triclosan in estuarine settings

Environmentally friendly analysis of emerging contaminants by pressurized hot water extraction–stir bar sorptive extraction–derivatization and gas chromatography–mass spectrometry

Contact Info

Product

Resources

About