Tricolor dual sensor for ratiometrically analyzing potassium ions and dissolved oxygen

Liu, Hongtian; Ning, Juewei; Song, Guoyong; Sun, Xiaohui; Su, Fengyu; Li, Pengfei; Tian, Yanqing

doi:10.1016/j.saa.2020.118155

Cited by 11 publications

(9 citation statements)

References 40 publications

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“…To investigate cytoplasmic K + dynamics in growing root hairs with high spatial and temporal resolution, a newly developed highly selective K + fluorescent sensor, NK3, was used in this study ( Figure S1 ) [ 17 ]. The optimal NK3 concentration was first determined by treating five-day-old green seedlings of Col-0 with different concentrations of NK3 and imaging under a Zeiss LSM 880 confocal microscope ( Figure S2 ).…”

Section: Resultsmentioning

confidence: 99%

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Close Temporal Relationship between Oscillating Cytosolic K+ and Growth in Root Hairs of Arabidopsis

Sun

Qiu

Peng

et al. 2020

IJMS

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Root hair elongation relies on polarized cell expansion at the growing tip. As a major osmotically active ion, potassium is expected to be continuously assimilated to maintain cell turgor during hair tip growth. However, due to the lack of practicable detection methods, the dynamics and physiological role of K+ in hair growth are still unclear. In this report, we apply the small-molecule fluorescent K+ sensor NK3 in Arabidopsis root hairs for the first time. By employing NK3, oscillating cytoplasmic K+ dynamics can be resolved at the tip of growing root hairs, similar to the growth oscillation pattern. Cross-correlation analysis indicates that K+ oscillation leads the growth oscillations by approximately 1.5 s. Artificially increasing cytoplasmic K+ level showed no significant influence on hair growth rate, but led to the formation of swelling structures at the tip, an increase of cytosolic Ca2+ level and microfilament depolymerization, implying the involvement of antagonistic regulatory factors (e.g., Ca2+ signaling) in the causality between cytoplasmic K+ and hair growth. These results suggest that, in each round of oscillating root hair elongation, the oscillatory cell expansion accelerates on the heels of cytosolic K+ increment, and decelerates with the activation of antagonistic regulators, thus forming a negative feedback loop which ensures the normal growth of root hairs.

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Section: Resultsmentioning

confidence: 99%

“…NK3 is a newly developed small-molecule fluorescent K + sensor ( Figure S1 ) [ 17 ]. The 2 mM stock solutions of NK3 were prepared in DMSO.…”

Section: Methodsmentioning

confidence: 99%

Close Temporal Relationship between Oscillating Cytosolic K+ and Growth in Root Hairs of Arabidopsis

Sun

Qiu

Peng

et al. 2020

IJMS

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“…The synthesis of the hydroxyl-terminated K + sensor (KS) was given in our previous report. 18 2.2. Instruments.…”

Section: Methodsmentioning

confidence: 99%

“…Challenges for development of well-performed fluorescent K + sensors are mainly from the influence of competing metal ions with comparable ion radius to K + , including Na + , Mg 2+ , Ca 2+ , and so on. Minta and Tsien 3 reported the first commercially available K + sensor PBFI (potassium-binding benzofuran isophthalate) based on benzofuranyl fluorophore and diaza- [18]-crown-6 ether, although it suffered some limitations from the interference of competing Na + . In 2003, He et al 4 constructed a triazacryptand (TAC) platform as the K + recognition unit and successfully overcame the interference of Na + .…”

Section: Introductionmentioning

confidence: 99%

“…In our previous work, we successfully synthesized a highly selective molecular K + probe (KS, Scheme 1) which was terminated with a hydroxyl group. 18 The KS was also used to investigate the relationship between the growth rate and cytosolic K + of root hairs of Arabidopsis. 25 However, the water solubility of the KS itself is poor and thus, all the titration tests are carried out with the aid of surfactants such as cetyltrimethylammonium bromide (CTAB), which causes some difficulties for imaging live cells or biological tissues.…”

Section: Introductionmentioning

confidence: 99%

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Rational Design of a Polymer-Based Ratiometric K⁺ Indicator for High-Throughput Monitoring Intracellular K⁺ Fluctuations

Ning

Liu

Sun

et al. 2021

ACS Appl. Bio Mater.

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Highly selective fluorescent K + sensors are of great importance for monitoring K + fluctuations in various biological processes. In particular, highly efficient ratiometric K + sensors that can emit in dual wavelengths and facilitate the quantitative determination of K + are highly anticipated. Herein, we present the first polymer-based ratiometric fluorescent K + indicator (PK1) for quantitatively detecting K + in aqueous solutions and high-throughput monitoring K + fluctuations in living cells. PK1 was synthesized by conjugating a small molecular K + probe and a red emission reference dye to a hydrophilic polymer skeleton. The newly synthesized PK1 can form highly stable nanoparticles in aqueous solutions and work in 100% water without the aid of any organic solvents or surfactants. PK1 is sensitive to K + with a fluorescence enhancement of sevenfold after interactions with K + at 1000 mM and inert to other metal ions, physiological pH, or dye concentration vibrations. More importantly, the fluorescence intensity ratio at 572 and 638 nm is linearly correlated with log [K + ] in the range of 2−500 mM (R 2 = 0.998), which will facilitate the quantitative detection of K + . Practical application of PK1 in detecting different K + -rich samples demonstrates its great potential in quantitative detection of K + . PK1 can be quickly internalized by live cells and shows no obvious cytotoxicity. We also demonstrate that PK1 could be used for monitoring K + fluctuations under different stimulations by using a confocal microscope and especially a microplate reader, which is high throughput and time saving. The rational design of PK1 will broaden the design concept of ratiometric fluorescent K + sensors and facilitate the quantitative detection of K + .

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Development of a molecular K+ probe for colorimetric/fluorescent/photoacoustic detection of K+

Ning

Lin

et al. 2020

Anal Bioanal Chem

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Tricolor dual sensor for ratiometrically analyzing potassium ions and dissolved oxygen

Cited by 11 publications

References 40 publications

Close Temporal Relationship between Oscillating Cytosolic K+ and Growth in Root Hairs of Arabidopsis

Close Temporal Relationship between Oscillating Cytosolic K+ and Growth in Root Hairs of Arabidopsis

Rational Design of a Polymer-Based Ratiometric K⁺ Indicator for High-Throughput Monitoring Intracellular K⁺ Fluctuations

Development of a molecular K+ probe for colorimetric/fluorescent/photoacoustic detection of K+

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Tricolor dual sensor for ratiometrically analyzing potassium ions and dissolved oxygen

Cited by 11 publications

References 40 publications

Close Temporal Relationship between Oscillating Cytosolic K+ and Growth in Root Hairs of Arabidopsis

Close Temporal Relationship between Oscillating Cytosolic K+ and Growth in Root Hairs of Arabidopsis

Rational Design of a Polymer-Based Ratiometric K+ Indicator for High-Throughput Monitoring Intracellular K+ Fluctuations

Development of a molecular K+ probe for colorimetric/fluorescent/photoacoustic detection of K+

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Rational Design of a Polymer-Based Ratiometric K⁺ Indicator for High-Throughput Monitoring Intracellular K⁺ Fluctuations