Trigeminal, vagal, and spinal projections of supramedullary cells in the puffer fish, <i>Takifugu niphobles</i>

Funakoshi, Kengo; Abe, Toshio; Kishida, Reiji

doi:10.1002/cne.903580408

Cited by 19 publications

(14 citation statements)

References 34 publications

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“…In these areas, the NADPH-diaphorase activity may be related to NO production by intrinsic NADPH-diaphorase-positive neurons in relation to the afferent fibers as suggested by Derer and Derer [1993] for the ontogenesis of mouse forebrain. NADPH-diaphorase activity was also demonstrated in the vagal afferent pathway of the adult dogfish Triakis scyllia [Funakoshi et al, 1997], and in Tilapia supramedullary neurons, that in the puffer fish [Funakoshi et al, 1995], project several peripheral processes into the sensory trigeminal, vagal and spinal nerves.…”

Section: Discussionmentioning

confidence: 95%

Development of NADPH-Diaphorase Activity in the Central Nervous System of the Cichlid Fish, Tilapia mariae

Villani

1999

Brain Behav Evol

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The distribution of nicotinamide adenine dinucleotide phosphate (NADPH)-diaphorase activity was studied in the cichlid fish Tilapia mariae during ontogenesis by the histochemical reaction of NADPH-diaphorase that indicates, in aldehyde-fixed tissue, the presence of nitric oxide synthase, which is the enzyme responsible for nitric oxide production. The first appearance of NADPH-diaphorase-positive neurons has a striking bilateral symmetry and occurs 20 h after fertilization (stage 8) in the olfactory placodes and in the neural tube where two clusters of positive neurons were seen in the diencephalon and in the rhombomere r4 of the hindbrain. Two days after fertilization (stage 10), the clusters of positive neurons showed labeled axons. The two longitudinal fiber bundles that arose from the diencephalic positive neurons ran caudally in the tract of the postoptic commissure. At stage 12 (3.5 days after fertilization), new populations of NADPH-diaphorase-positive neurons appeared in the telencephalon, in some diencephalic nuclei, and in the hypothalamus. Several trigeminal motor neurons showed strong NADPH-diaphorase activity, whereas the optic tectum and cerebellum were completely free of enzymatic activity. In the hindbrain, clusters of positive neurons were seen in the octavolateral region and in the region defined by the exit of the vagus nerve. In the cervical spinal cord, some ventral putative motor neurons were labeled. At stage 14 (5.5 days after fertilization), several periventricular neurons of the optic tectum and some neurons of the cerebellar lamina were labeled. Dorsal neurons, including a few large superficial neurons were also labeled in the cervical spinal cord. NADPH-diaphorase activity was seen in the neuropil area of the telencephalon, the target of olfactory inputs, and in the sensory dorso-lateral area of the spinal cord.

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Section: Discussionmentioning

confidence: 95%

Development of NADPH-Diaphorase Activity in the Central Nervous System of the Cichlid Fish, Tilapia mariae

Villani

1999

Brain Behav Evol

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“…Calcitonin in Fugu is found in the supermedullary neurones (Fig. 6C) and in a related puffer fish (Takifugu niphobles) these project processes (axons) into several nerves including the trigeminal, vagus and spinal accessories (Funakoshi et al 1995). Other evidence suggests that these neurones may have synaptic connections with the central nervous system (Funakoshi et al 1996) and also with the skin, possibly being involved in chromatophore control.…”

Section: Discussionmentioning

confidence: 96%

Calcitonin: characterisation and expression in a teleost fish, Fugu rubripes

Clark

Bendell²,

Power³

et al. 2002

Journal of Molecular Endocrinology

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The present report describes the structure and expression of the calcitonin gene in Fugu rubripes. It is composed of 4 exons and 3 introns. Splicing of exons 1, 2 and 3 generates the calcitonin pre-proprotein, while splicing of exons 1, 2 and 4 generates calcitonin gene-related protein (CGRP). Exons 1 and 2 encoding the signal sequence and the N-terminal peptide are common in both the gene products and this gene organisation has been conserved in human, rat, chicken and salmon. The gene environment around calcitonin in Fugu has been poorly conserved when compared with human, apart from a small gene cluster. The calcitonin gene in Fugu has a widespread tissue distribution but it is most highly expressed in the brain. The abundance of gene expression in the ultimobranchial gland and the pituitary indicates that these are important sites of production and that the peptide is probably secreted into the circulation and/or acts as a paracrine or autocrine controlling factor. Whilst the function of calcitonin in fish is still largely unknown, the distribution described here suggests that one of the potential functions may be as a neuropeptide.

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“…The discovery of vocal CPG input to supramedullary neurons was unexpected, as this connection was not found in midshipman following extensive study of larval through adult stages Knapp et al, 1999]. Supramedullary neurons are proposed to innervate mucous glands distributed across the body surface via several cranial nerves [Bennett et al, 1959;Funakoshi et al, 1995Funakoshi et al, , 1998]. The functional significance of this vocal input remains to be shown, although increased mucous secretion might serve a protective function (e.g.…”

Section: Discussionmentioning

confidence: 99%

Vocal Behavior and Vocal Central Pattern Generator Organization Diverge among Toadfishes

Chagnaud

Bass

2014

Brain Behav Evol

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Among fishes, acoustic communication is best studied in toadfishes, a single order and family that includes species commonly known as toadfish and midshipman. However, there is a lack of comparative anatomical and physiological studies, making it difficult to identify both shared and derived mechanisms of vocalization among toadfishes. Here, vocal nerve labeling and intracellular in vivo recording and staining delineated the hindbrain vocal network of the Gulf toadfish Opsanus beta. Dextran-biotin labeling of the vocal nerve or intracellular neurobiotin fills of motoneurons delineated a midline vocal motor nucleus (VMN). Motoneurons showed bilaterally extensive dendritic arbors both within and lateral to the paired motor nuclei. The motoneuron activity matched that of the spike-like vocal nerve motor volley that determines the natural call duration and frequency. Ipsilateral vocal nerve labeling with biocytin or neurobiotin yielded dense bilateral transneuronal filling of motoneurons and coextensive columns of premotor neurons. These premotor neurons generated pacemaker-like action potentials matched 1:1 with vocal nerve and motoneuron firing. Transneuronal transport further revealed connectivity within and between the pacemaker-motor circuit and a rostral prepacemaker nucleus. Unlike the pacemaker-motor circuit, prepacemaker firing did not match the frequency of vocal nerve activity but instead was predictive of the duration of the vocal nerve volley that codes for call duration. Transneuronally labeled terminal-like boutons also occurred in auditory-recipient hindbrain nuclei, including neurons innervating the inner ear and lateral line organs. Together with studies of midshipman, we propose that separate premotor populations coding vocal frequency and duration with direct premotor coupling to auditory-lateral line nuclei are plesiomorphic characters for toadfishes. Unlike in midshipman, transneuronal labeling in toadfishes reveals an expansive column of pacemaker neurons that is weakly coupled to prepacemaker neurons, a character that likely depends on the extent of gap junction coupling. We propose that these and other anatomical characters contribute to neurophysiological properties that, in turn, sculpt the species-typical patterning of frequency and amplitude-modulated vocalizations.

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Trigeminal, vagal, and spinal projections of supramedullary cells in the puffer fish, Takifugu niphobles

Cited by 19 publications

References 34 publications

Development of NADPH-Diaphorase Activity in the Central Nervous System of the Cichlid Fish, Tilapia mariae

Development of NADPH-Diaphorase Activity in the Central Nervous System of the Cichlid Fish, Tilapia mariae

Calcitonin: characterisation and expression in a teleost fish, Fugu rubripes

Vocal Behavior and Vocal Central Pattern Generator Organization Diverge among Toadfishes

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