Triggering of drug release of particles in hair follicles

Abstract

Particulate drug delivery via hair follicles represents a promising concept, although requirements are high. This process must be realized at the desired depth and at the appropriate time, due to the fact that the particles themselves are not able to overcome the follicular skin barrier.In the present study, a novel triggering concept for the release of a model drug from the delivering particles is presented basing on the application of two different particle types of the same size, where one particle type is the drug carrier, and the second one is loaded with a protease. The latter one is supposed to interact with the drugcarrying particles to trigger the drug release. A mixture of both particles was applied on porcine skin samples, followed by follicular analysis. As controls, the particles were applied unaided without protease, and one skin area remained untreated. The investigations revealed that the protease was able to release the model drug from the delivering particles in significant depths within the hair follicle (866 ± 62 nm).Additionally, an uptake of the model drug in the sebaceous gland was observed after release providing a promising novel approach for the development of treatment strategies of different skin diseases.

Hair follicles as a target structure for nanoparticles

“…6, the protease was tagged with rhodamine and included in CaCO 3 porous silicon particles that were applied together with BSA particles loaded with the model drug°uorescein isothiocyanate on to porcine ear skin. 32 This enabled the detection of the e±cient release of the model drug inside the hair follicle down to a depth of 900 m. Thus, this two-component system proved to be an e±cient tool for transferring highly concentrated drugs into the hair follicles and their localized release. 32 A third experiment focused on the release of the model drug by an external trigger signal.…”

“…32 This enabled the detection of the e±cient release of the model drug inside the hair follicle down to a depth of 900 m. Thus, this two-component system proved to be an e±cient tool for transferring highly concentrated drugs into the hair follicles and their localized release. 32 A third experiment focused on the release of the model drug by an external trigger signal. For this purpose, the drug-loaded particles were coated with gold.…”

Biocompatibility and characterization of polyglycerol-based thermoresponsive nanogels designed as novel drug-delivery systems and their intracellular localization in keratinocytes

“…By contrast, polymeric particles can be adjusted to their field of operation and can be loaded for drug delivery (Mak et al, 2012). The tNGs utilized in this study were synthesized from dPG cross-linked by thermoresponsive polymers tPG or pNIPAM (Giulbudagian et al, 2014).…”

(Phospho)lipid-based Nanosystems for Skin Administration

“…(Trans)dermal route and recently revived hair follicles targeting [193][194][195][196][197][198][199]. Multidisciplinary approaches in nanodermatology are needed to understand the mechanisms of interactions between nanomaterials and the skin, particularly diseased skin.…”