Triiodothyronine Has No Enhancement Effect on the Osteogenic or Chondrogenic Differentiation of Equine Adipose Tissue Stem Cells

Spila, Débora de Oliveira; Maranhão, Renata de Pino Albuquerque; Ocarino, Natália de Melo; Lima, Jorge Tiburcio Barbosa de; Melo, Fabrício Gomes; Boeloni, Jankerle Neves; Serákides, Rogéria

doi:10.1016/j.jevs.2019.102895

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“…In our study, the presence of thyroid hormone did not modify the expressions of aggrecan, Col II or Col X in ASCs in any of the evaluated periods. This finding corroborates in part an in vitro study that showed that hormone treatment also does not affect expression of aggrecan of BM-MSC (ASSIS et al, 2018), and does not affect chondrogenic matrix percentage of ASC (SPILA et al, 2019). In addition, in vitro hormone treatment (30 ng/mL T4) did not alter the Col II expression at 21 days of culture of chondrocytes in the terminal differentiation phase (OKUBO & REDDI, 2003).…”

Section: Discussionsupporting

confidence: 86%

Triiodothyronine does not influence in vitro chondrogenic differentiation of adipose tissue-derived stem cells from young female rat

et al. 2022

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The objective of this study was to investigate the in vitro action of triiodothyronine (T3) on the chondrogenic differentiation of adipose tissue-derived stem cells (ASCs) of female rats, with different time periods and doses. ASCs were extracted from female Wistar rats and were cultured in chondrogenic medium with and without the presence of T3. Five groups were established: 1) ASCs without T3; and 2,3,4,5) ASCs with 0.01, 1, 100 and 1,000 nM T3, respectively). After 7, 14 and 21 days, cell morphology, chondrogenic matrix formation, and expression of Sox9, aggrecan, collagen II, and collagen X were evaluated. The Student-Newman-Keuls test was used. ASCs showed CD54, CD73, and CD90 before chondrogenic differentiation. The hormone treatment did not alter chondrogenic matrix formation, Sox9 expression at 14 or 21 days, or expression of collagen II or collagen X at any time. However, the 0.01, 1, and 1000 nM T3 doses decreased Sox9 expression at 7 days. In conclusion, chondrogenic differentiation of ASCs of female rats is not influenced by T3.

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Section: Discussionsupporting

confidence: 86%